B.Sc. V-Semester (CBCS) Examination, November / December 2018

Subject : Bio-Technology

(Molecular Biology and Recombinant DNA Technology)

Paper -V : (DSC)

Time : 3 Hours Max. Marks: 60

PART - A (5 x 3 = 15 Marks)

(Short Answer Type)

Note : Answer any FIVE of the following questions.

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1. Explain reverse transcription.
2. What is CAP-cAMP system?
3. Write a short note on transcription machinery.
4. Describe mating types in yeast.
5. Write about PCR and its applications.

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6. Explain Southern, northern and western blotting techniques.
7. Detail Zoo blot.
8. Describe cosmid and phagemid vectors.

PART - B (3 x 15 = 45 Marks)

(Essay Answer Type)

Attempt ALL the questions

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1. (a) Detail all the steps in transcription.
   (b) Explain the process of translation.
2. (a) Describe the structure of eukaryotic gene.
   (b) Explain in detail about the role of transcription factors.
3. (a) Describe the applications of recombinant DNA technology.

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