Download SGBAU BSc 2019 Summer 4th Sem Biotechnology Genetic Engineering n Microbial Biotechnology Question Paper

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B.Sc, (Part-II) Semester-IV Examination

4S : BIOTECHNOLOGY

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(Genetic Engineering and Microbial Biotechnology)

Time : Three Hours] [Maximum Marks : 60

Note :— (1) ALL questions are compulsory.

(2) Draw well labelled diagrams wherever necessary.

1. (A) Fill in the blanks :
   1. Transfer of DNA on Nitrocellulose membrane is called __________.

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   2. __________ is used as Antidiabetic protein.
   3. Extraction of Metal ions with the help of Micro-organisms is called __________.
   4. Lac Z gene of Lac Operon codes for __________.
2. (B) Choose the correct alternative :
   1. Which one is not a termination codon ?
      1. UAA

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      2. UAG
      3. AUG
      4. UGA
   2. PCR was invented by :
      1. Watson & Crick

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      2. Kary Mullis
      3. Jacob & Monad
      4. Willkins
   3. Which is the main component of Biogas ?
      1. CO₂

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      2. H₂
      3. O₂
      4. CH₄
   4. Which among these is not a biofertilizer ?
      1. Rhizobium

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      2. Agrobacterium
      3. Azospirillum
      4. Azotobacter

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3. (C) Answer in one sentence each :
   1. What is Transposon ?
   2. Give long form of CSTR.
   3. Define fermentation
   4. What are bacteriophages ?

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4. Describe Watson & Crick double helical model of DNA. 12

   OR

   What is Replication ? Describe the mechanism of DNA replication in Eukaryotes. 12
5. Explain :
   1. Components of Lac operon 4
   2. Transcriptional Bubble 4
   3. Britton & Davidson model. 4

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   OR

   1. Structure of mRNA
   2. Post translational modification of protein.
6. Describe :
   1. Southern Blotting 4

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   2. Plasmid 4
   3. DNA manipulating enzymes 4

   OR

   1. Bacteriophage 3
   2. Genomic DNA library 4

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   3. PCR. 4
7. Describe :
   1. Insulin 4
   2. Recombinant vaccines 4
   3. Amino acids. 4

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   OR

   1. Interferon 4
   2. Dextran 4
   3. Erythropoietin. 4

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8. Explain :
   1. CSTR Bioreactor 6
   2. Alcohol fermentation 6

   OR

   1. Batch & continuous fermentation 6
   2. Penicillin fermentation 6

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9. Describe :
   1. Biogas 4
   2. Rhizobium as biofertilizer 4
   3. Bioremediation 4

   OR

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   1. Bioleaching 4
   2. Biopesticide 4
   3. Xenobiotic compounds. 4

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