Download SGBAU BSc 2019 Summer 5th Sem Biochemistry Molecular Biology n Biotechnology Question Paper

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B.Sc. (Part—IIT) Semester—V Examination

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BIOCHEMISTRY
(Molecular Biology and Biotechnology)

Time : Three Hours] [Maximum Marks : 80

Note :— (1) All questions are compulsory and carry equal marks except Q. No. 1 which carries 8 marks.
(2) Draw neat and labelled diagram wherever necessary.

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1. (A) Fill in the blanks :
   1. Nucleic acids are polymers of __________.
   2. During translation the enzyme involved in activation of amino acid is __________.
   3. __________ is a DNA joining enzyme.
   4. The process of RNA synthesis is known as __________.
   (B) Choose the correct alternative :—
   1. The nitrogenous base not present in DNA is :
      1. Adenine

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      2. Guanine
      3. Uracil
      4. Thymine
   2. Which one of the following is not involved in initiation of translation ?
      1. IF₁

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      2. IF₂
      3. EF-TU
      4. TF₁
   3. Which of the following subunit of RNA polymerase searches a promoter sequence :
      1. Sigma

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      2. Alpha
      3. Beta
      4. Delta
   4. The number of base pairs present in each turn of B-DNA are :
      1. 9

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      2. 10
      3. 11
      4. 12
   (C)
   1. Define transgenic plants.

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   2. Define Primary cell culture.
   3. Define translation.
   4. Define Totipotency.
2. (a) Describe in brief Watson and Crick double helical model of DNA.
   

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   (b) Explain features of Viral genome.
   (c) Describe current version of central dogma of molecular genetics.
   OR
   (p) Explain structure and function of tRNA.
   (q) Discuss features of Prokaryotic genomes.
   

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   (r) Discuss in brief Hershey and Chase experiment.
3. Describe with suitable diagram initiation, elongation and termination of transcription. OR Explain the role of enzyme and proteins involved in DNA replication with a suitable diagram and add a note on experimental evidence for semiconservative DNA replication.
4. Describe in detail translation in Prokaryotes. OR Discuss in detail lac operon and add a note on overlapping genes.
5. (a) Explain Sanger method for sequencing of DNA.
   (b) Explain the role of DNA ligase in Recombinant DNA technology.
   

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   (c) Describe in brief Northern blotting.
   OR
   (p) Describe COSMID vectors.
   (q) Discuss electroporation.
   (r) Explain shuttle vectors.

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6. (a) Describe growth kinetics of cell in culture.
   (b) Explain importance of growth factors of serum.
   (c) Discuss History of animal cell culture.
   OR
   (p) Discuss in brief application of animal cell culture in study of gene expression.
   

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   (q) Explain organ culture.
   (r) Describe in brief primary and secondary cell culture.
7. (a) Describe media preparation and composition for plant tissue culture.
   (b) Discuss any four practical applications of Plant tissue culture.
   (c) Discuss ovule culture.
   

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   OR
   (p) Give any four practical applications of genetic transformation in plants.
   (q) Explain suspension culture.
   (r) Describe in brief in vitro pollination and fertilization.

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