Download SGBAU BSc 2019 Summer 5th Sem Biochemistry Molecular Biology n Biotechnology Question Paper

Download SGBAU (Sant Gadge Baba Amravati university) BSc 2019 Summer (Bachelor of Science) 5th Sem Biochemistry Molecular Biology n Biotechnology Previous Question Paper

AW?1752
B.Sc. (l?art?III) Semestcr?V Examination
BIOCHEMISTRY
(Molecular Biology and Biotechnology)
Time : Three Hours] [Maximum Marks : 80
Note 2? (1) All questions are compulsory and carry equal marks except Q. No. 1 which
carries 8 marks.
(2) Draw neat and labeled diagram wherever necessary.
l. (A) Fill in the blanks :
(i) Nucleic acids are polymers of_ _ . 1/2
(ii) During translation the enzyme involved in activation 01' amino acid is _ _ . 1/:
(iii) is a DNA joining enzyme. I/2
(iv) The process of RN A synthesis is known as -7 _ 1/2
(B) Choose the correct alternative z~
(i) The nitrogenous base not present in DN A is : 'l":
(A) Adenine (B) Guzmine ?
(C) Uracil CD) Thyminc
(ii) Which one of the following is not involved in initiation of translation ?2 V2
(A) IF 1 (8) [F2
(C) EF-TU (D) IF3
(iii) Which of the following subunit of RNA polymerase searches a promoter sequence :
'/z
(A) Sigma (B) Alpha
(C) Bela (D) Delta
(iv) The number of base pairs present in each turn of B-DNA arc : 1/2
(A) 9 (B) 10
(C) 11 (D) 12
(C) (1) Dc?ne transgenic plants. 1
(ii) De?ne P?mury cell culture. 1
(iii) De?ne translation. ' 1
(iv) De?ne Totipotency. l
2. (a) Describe in brief Watson and Crick double helical model of DNA. 4
0)) Explain features usiral genome. 4
(c) Describe current version of central dogma ot?molccular genetics. 4
0R
YBC?l 5320 1 (Contd.)

(p)
(q)
(r)
la)
Explain structurc and function ofr-RNA.
Discuss features 01' P:Ok:lf}u[ic gemmtes.
Discuss in bricl?llcrshe) and Chase experiment.
Describe wit.) suitable diagram init ation, elongation and termination of transcription.
OR
12
Explain the role of enzyme and prmeins involved in DNA replication wth a suitable diagram and
add a note on experimental ex'idcuae for scmiconserx?ative DNA replication.
4. Describe in detail translation 11 Prukaryotcs
0R
Discuss in detail lac operon and :Lid a note on overlapping genes.
5. (:1) Explain Sanger mclhod for sequencing ofDNA.
(b) Explain the role of DNA li;;_1:: in Recombinant I').\'A technology.
(C) Describ: in brici'Nonhel n N(?tting.
()R
(p) Describe COSMIT) \?ectvws.
(q) Discuss electroporution.
(r) Explain shuttle Vectors.
6 (u) Describe growth kinetics ofcrll in culture.
(b?) Explain importance ofgrm-?th factors ofxrum.
(c) Discuss Histmy of animal ccll culture,
OR
(p) Discuss in bricfapplication 01 animal cell culture in study of gene expression.
(q) Explain organ culture.
(r?) Describe in bricz'priman' mid iCCOIldLu?) C311 culture.
7 (:1) Describe media prcpmlicn and composition for plant tissue culturc.
(b) Discuss any four p-nctical urpisalions of'PIant tissue CLll? urc.
(c) Discuss ovulc culture.
0R
(p) Give any four practical a WpIicmions of genetic transformation in p. ants.
((1) Explain suspension culturs.
(r) Describe in brief in \ritro pol] ?rza-t'an and I?erzilizatimz.
YBC?ISSL?O 7
12
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