Download SGBAU BSc 2019 Summer 6th Sem Biotechnology Plant Biotechnology Question Paper

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B.Sc. (Part-III) Semester—VI Examination

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6S : BIOTECHNOLOGY (R/Y)
(Plant Biotechnology)

Time : Three Hours] [Maximum Marks : 80

Note :— (1) All questions are compulsory.

(2) Draw neat and well labelled diagrams wherever necessary.

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1. (A) Fill in the blanks :
   1. The movement of ions across cell membrane without need of cellular energy is __________.
   2. Exchange of CO₂ and O₂ in plants takes place through __________.
   3. T-DNA is found in __________ plasmid.
   4. Use of electric pulse for gene transfer is called __________.
   (B) Multiple choice questions :
   1. Hormone responsible for Apical Dominance is :
      1. (a) Auxin

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      2. (b) Cytokinin
      3. (c) Ethylene
      4. (d) GA
   2. Aseptic inoculation of explant on Nutrient medium requires :
      1. (a) Centrifuge

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      2. (b) Growth chamber
      3. (c) Laminar Air flow
      4. (d) Autoclave
   3. Enzymes required for protoplast isolation are :
      1. (a) Invertases

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      2. (b) Cellulase and Pectinase
      3. (c) Nucleases
      4. (d) Zymase
   4. Triploids are produced by :
      1. (a) Pollen culture

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      2. (b) Anther culture
      3. (c) Endosperm culture
      4. (d) All of the above
   (C) Answer in one sentence each :
   1. Define Organogenesis.

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   2. What is Somaclonal variation ?
   3. Give the role of Autoclave in Tissue culture.
   4. What is Geotropism ?
2. Describe :
   1. (a) Growth curve.

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   2. (b) Structure of stomata.
   3. (c) Geotropism.
   OR
   1. (d) Apical dominance
   2. (e) Radiant energy.
   3. (f) Phototropism.

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3. Explain in detail the physiological roles of Auxin. OR Explain in detail the physiological roles of Gibberellins.
4. Explain in brief :
   1. (a) Practical applications of Tissue culture.
   2. (b) Growth chamber.
   3. (c) Composition of MS medium.

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   OR
   1. (d) Design of Tissue Culture Laboratory.
   2. (e) Commercialization of Tissue Culture.
   3. (f) Autoclave.
5. Describe :
   1. (a) Micropropagation.

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   2. (b) Hardening methods of Tissue cultured plants.
   3. (c) Embryo rescue.
   OR
   1. (d) Anther culture.
   2. (e) Applications of somaclonal variation.
   3. (f) Callus culture.

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6. Describe techniques of Single cell suspension culture and its applications. OR Describe protoplast isolation and regeneration in detail.
7. Describe :
   1. (a) Ti-plasmid.
   2. (b) Somatic hybridization
   3. (c) Gene gun method of gene transfer.

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   OR
   1. (d) Electroporation
   2. (e) Markers for selection of hybrid cells
   3. (f) Cybrids.

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