Download SGBAU BSc 2019 Summer 6th Sem Botany Molecular Biology n Biotechnology Question Paper

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B.Sc. (Part-II) Semester—VI Examination

BOTANY

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(Molecular Biology and Biotechnology)

Time : Three Hours] [Maximum Marks : 80

Note :— (1) There are seven questions in all.

(2) Q.1 is compulsory and carries 8 marks.

(3) Q.2 to Q.7 carry equal marks.

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(4) Draw neat and well labelled diagrams wherever necessary.

1. (A) Fill in the blanks :
   1. The backbone of DNA is made up of __ Bonds.
   2. The organelle involved in Protein synthesis is __ .
   3. Lac Z gene of Lac operon encodes ___ enzyme.
   4. GMO stands for __ .

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   (B) Choose the correct alternative (MCQ) :
   1. Phage DNA used by Hershey and Chase is radio labelled with :
      1. ³⁵S
      2. ³²P
      3. ¹⁴CO
      4. ³H

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   2. Gene as a unit of mutation terms as :
      1. Recon
      2. Muton
      3. Cistron
      4. Nucleotide

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   3. Gene Battery Model is given by :
      1. Jacob and Monad
      2. Crick
      3. Britton and Davidson
      4. Emil Fischer

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   4. For aseptic transfer of explant on nutrient medium needs :
      1. Incubator
      2. Centrifuge
      3. Oven
      4. Laminar Air flow

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   (C) Write answer in one sentence each :
   1. Define plasmid.
   2. Give the site of protein synthesis in the cytoplasm.
   3. Which is initiation codon ?
   4. What is Protoplast ?

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2. Explain :
   1. Griffin's experiment.
   2. Ac-Ds system.
   3. Nucleosome model.
   OR
   1. Chemical composition of DNA.
   2. Replication fork.

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   3. Satellite DNA.
3. Describe the different types of RNA. OR Describe transcription and m-RNA processing in Eukaryotes.
4. Describe operon concept with special reference to Lac Operon. OR Describe Primary, Secondary, Tertiary and Quaternary structures of proteins.
5. Explain :
   1. Ti plasmid.

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   2. Genomic DNA library
   3. Restriction endonucleases.
   OR
   1. Phages as cloning vector.
   2. cDNA Library.
   3. PCR.

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6. Explain :
   1. Autoclave.
   2. Micropropagation
   3. Role of hormones in Tissue culture.
   OR
   1. Laminar Air flow.

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   2. Totipotency.
   3. Callus culture.
7. Describe in brief :
   1. BT-cotton.
   2. Edible vaccines.

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   3. Protoplast culture.
   OR
   1. Somatic hybridization.
   2. Alcohol production by fermentation.
   3. Synthetic seeds.

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