B.Sc. (Part?lll) Semester?Vl Examination
ZOOLOGY
(Molecular Biology and Biotechnology)
Time : Three Hours] [Maximum Marks : 80
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Note :~(1) All the questions are compulsory.(2) Question No. 1 carries 8 marks.
(3) Question Nos. 2 to 7 carry 12 marks each.
(4) Illustrate your answers with suitable diagrams wherever necessary.
1. (a) Fill in the blanks :
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(i) The following nucleic acid has a left handed helix ? __. 1/1(ii) Gcnc as unit of Mutation is called as _. [/2
(iii) In DNA guanine always pairs with _,_- '/:
(iv) ELISA is based 0n _. '/2
(b) Choose the correct alternatives from the following :
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(v) How many codons are called to code for all 20 essential amino acids ?3 V:(a) 20 (b) 4
(c) 61 (d) 60
(vi) Transcription takes place in : V2
(:1) Matrix (b) Nucleus
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(c) Cystol (d) Cytoplasm(vii) The anticodon region is ah important structural component of __. '/2
(a) m-RNA (b) DNA
(c) r-RNA ' (d) t-RNA
(viii)Thc antibodies are *. ?/2
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(a) Carbohydrates (b) Proteins(c) Lipids (d) Germs
(c) Answer in one sentence each :
(ix) What is point mutation ? l
(x) What is Lambda phage vector ? 1
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(xi) What is bacteriophage '3 l(xii) What are introns ? l
2. Describe mitochondrial DNA. 12
OR
Explain the structural characteristics of clover leaf model of tRNA and its functions.
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3. Describe the mechanism of DNA Replication. 12OR
Describe overlapping gene and jumping gene.
YBC?IS339 1 (Contd.)
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YBCAttempt the following :
(a) Features of Genetic cnde?
(b) Britten Davidson Model.
(0) Transcription of mRNA.
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OR(d) Draw a well-labclled diagram? of Lac-operun model.
(6) 'I?ranslatinn.
(1?) Gene regulation
Explain the following :
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(g) Frame shift mutations.(h) Southern blotting.
(i) Polymerase (?hain Rcacmms (PCR).
0R
(j) Signi?c?mce of Mutation.
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(k) Westem blotting.(1) Applications of DNA ?ngerprinting.
lixplain the following :
(m) Plasmid as a vector.
(n) Somatic cell hybridimt'on.
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(,0) Cloning of genesOR
(p) Applications of monucionnl antibodies.
(q) Recombinant DNA 'I'cchnnlugy.
(r) Splicing of genes.
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Describe the following :(s) T-ccll receptors.
(0 Functions of Antibody.
(u) ELISA.
OR
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(v) llaplcns.(w) Innate Immunity.
(x) RM.
[5339 2
12
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525