Download SGBAU BSc 2019 Summer 2nd Sem Biochemistry Biophysical n Biochemical Techniques Question Paper

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B.Sc. (Part-I) Semester—II Examination

2S : BIOCHEMISTRY

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(Biophysical and Biochemical Techniques)

Time : Three Hours] [Maximum Marks : 80

Note :— All questions are compulsory and carry equal marks except Question No. 1 which carries 8 marks.

1. (A) Fill in the blanks :
   1. RIA stands for __________
   2. Movement of ions or molecules from a region of high concentration to low concentration is known as __________

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   3. Technique used for separation of protein on the basis of molecular weight is __________
   4. Negative logarithm of hydrogen ion concentration is __________
2. (B) Choose the correct alternative -
   1. Oxidation is defined as : (a) Addition of oxygen (b) Loss of hydrogen (c) Loss of electron (d) All of the above
   2. Ion exchange chromatography is based on : (a) Electrostatic attraction (b) Affinity (c) Adsorption (d) Partition

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   3. A solution which resists sudden change in pH is known as : (a) Buffer (b) Standard (c) Acidic (d) Basic
   4. In spontaneous reactions value of AG is : (a) Negative (b) Positive (c) Zero (d) None of the above
3. (C) Answer in ONE sentence :—
   1. Define redox potential.
   2. Define dialysis.

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   3. Define Spontaneous reaction.
   4. Define Beer’s Lambert Law.

1. (A) Describe first and second law of thermodynamics.
2. (B) Describe application of Gibbs free energy change in Biochemistry.

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3. (C) Explain standard free energy change in coupled reactions.

OR

1. (P) Describe various thermodynamics.
2. (Q) Explain relation between standard redox potential and free energy change.
3. (R) Explain the concept of Entropy.

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1. (A) Explain application of dialysis in Biochemistry.
2. (B) Describe measurement of pH by indicators.
3. (C) Comment on preparative ultracentrifugation.

OR

1. (P) Explain the concept of pH and buffers.

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2. (Q) Describe Handerson-Hasselbalch equation.
3. (R) Explain active and passive transport

1. Describe principle of :
   1. (A) Ion exchange chromatography.
   2. (B) Paper chromatography
   3. (C) Molecular sieve chromatography.

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OR

1. Explain applications of :
   1. (P) Ion exchange chromatography.
   2. (Q) Gas liquid chromatography
   3. (R) HPLC.

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1. Describe in detail Western blotting.

OR

1. Describe in detail SDS-PAGE and isoelectric focusing.

1. (A) Describe Beer Lambert’s Law.

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2. (B) Describe Principle of fluorometry.
3. (C) Explain principle of mass spectroscopy.

OR

1. (P) Describe applications of NMR.
2. (Q) Describe principle of ESR

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3. (R) Write in brief about flame photometry.

1. Explain in detail with suitable diagram principle and application of ELISA.

OR

1. Explain in detail isotopic tracer techniques and add a note on autoradiography.

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