Download PTU M.Tech. Bio Tech 2nd Semester 76048 APPLIED BIOTECHNOLOGY Question Paper

Download PTU. I.K. Gujral Punjab Technical University (IKGPTU) M.Tech. Bio Tech 2nd Semester 76048 APPLIED BIOTECHNOLOGY Question Paper.

1 | M-76048 (S35)-846

Roll No. Total No. of Pages : 01
Total No. of Questions : 08
M.Tech. (Bio Tech) (2018 Batch) (Sem.?2)
APPLIED BIOTECHNOLOGY
Subject Code : MTBT-202-18
M.Code : 76048
Time : 3 Hrs. Max. Marks : 60

INSTRUCTIONS TO CANDIDATES :
1.Attempt any FIVE questions out of EIGHT questions.
2.Each question carries TWELVE marks.

1. What is hybridization? Describe briefly northern, southern and fluorescence in situ
hybridization.
2. What are cloning and expression vectors? Describe briefly pBR 322 and puc ? based
vectors used in rDNA technology.
3. What are real time PCR, anchored PCR and inverse PCR? Describe the applications of
PCR in cloning, diagnostics and biomedical sequencing.
4. What are genomic and cDNA libraries? Describe the screening of libraries by nucleic
acid hybridization and phage display method.
5. Describe briefly TAP tagging DNA sequencing methods used in gene analysis and their
applications in medical science.
6. Describe briefly expression in E. coli, yeast and insect cells.
7. Discuss briefly molecular approaches to generate transgenic organisms and their
applications.
8. What is in vivo and ex vivo gene therapy? Describe briefly gene knockout analysis with
suitable examples.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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