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Roll No.
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Total No. of Questions : 08
Total No. of Pages : 01
M.Tech. (Bio Tech) (2018 Batch) (Sem.-2)
APPLIED BIOTECHNOLOGY
Subject Code : MTBT-202-18
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M.Code: 76048
Time: 3 Hrs.
Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
- Attempt any FIVE questions out of EIGHT questions.
- Each question carries TWELVE marks.
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- What is hybridization? Describe briefly northern, southern and fluorescence in situ hybridization.
- What are cloning and expression vectors? Describe briefly pBR 322 and puc ? based vectors used in rDNA technology.
- What are real time PCR, anchored PCR and inverse PCR? Describe the applications of PCR in cloning, diagnostics and biomedical.
- What are genomic and cDNA libraries? Describe the screening of libraries by nucleic acid hybridization and phage display method.
- Describe briefly TAP tagging, DNA sequencing methods used in gene analysis and their applications in medical.
- Discuss briefly expression in E. coli, yeast and insect cells.
- Describe briefly molecular approaches to generate transgenic organisms and their applications.
- What is in vivo and ex vivo gene therapy? Describe briefly gene knockout analysis with suitable examples.
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NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.
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1| M-76048
(S35)-846
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