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DUET 2019 PhD in Biochemistry Previous Queston Papers

Delhi University Entrance Test (DUET) 2019 PhD in Biochemistry Previous Queston Papers

This post was last modified on 19 June 2020

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DUET Last 10 Years 2011-2021 Question Papers With Answer Key || Delhi University Entrance Test conducted by the NTA

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DU PhD in Biochemistry

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Topic:- DU_J19_PHD_BIOCHEM

Which of the following statement about protein structures and functions is likely to be False?
1. Two proteins that share a significant degree of sequence similarity either with each other or with a third sequence also share an evolutionary origin and should share some structural features also
2. Two entirely different protein sequences from different evolutionary origins may fold into a similar structure
3. Ancient gene for a given protein structure may diverge in different species while maintaining the same basic structural features
4. Proteins that are members of the same family are likely to have same three-dimensional structures but different functions
Correct Answer :-

Two entirely different protein sequences from different evolutionary origins may fold into a similar structure
Which of the following amino acid is not involved in the composition of glutathione?
1. Glycine
2. Glutamine
3. Glutamate
4. Cysteine
Correct Answer :-

Glutamate

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Which of the following is NOT a change experienced by a typical cell committed for apoptosis?
1. Cell swells and ultimately bursts
2. Loss of mitochondrial membrane functions
3. Cytoskeleton collapses
4. DNA breaks into fragments
Correct Answer :-
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Cytoskeleton collapses
Which of the following techniques is the most suitable for detecting a metabolite labelled with 13C?
1. Mass spectrometry
2. Infra red spectroscopy
3. Nuclear magnetic resonance spectroscopy
4. Scintillation counting (detection of radioactivity)
Infra red spectroscopy
Which of the following properties is not associated with non-covalent interactions in proteins?
1. Involves a chemical bond
2. Important for secondary and tertiary structural integrity
3. Weak interactions
4. Interaction in space
Correct Answer :-

Interaction in space

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Which one of the following techniques will you use to identify more than 1000 differentially expressed genes in tumor tissues in one experiment?
1. RAPD
2. ChiP assay
3. Genome sequencing
4. Microarrays
Correct Answer :-
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RAPD
Which one of the following statements is correct?
1. In all L-amino acids, only the C-terminal carbon atom is chiral
2. Phosphatidyl choline isolated from biological membranes is optically active
3. Deoxyribose is optically active
4. The specific rotation of sucrose will be the sum of the specific rotations of D-glucose and D-fructose
Correct Answer :-

Deoxyribose is optically active
Which is true for size exclusion chromatography?
1. Sample volume affects the resolution of separation of proteins
2. Width of the column affects the resolution of separation of proteins
3. Only a mixture of five to ten proteins can be well separated
4. Length of the column does not affect the resolution of separation of proteins
Correct Answer :-

Length of the column does not affect the resolution of separation of proteins
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Insects such as Drosophila undergo three molts before becoming a pupa and undergoing metamorphosis. Molting, which is also called 'ecdysis', is controlled by what hormone?
1. Auxin
2. Ecdysone
3. Cytokinin
Correct Answer :-
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Ecdysone
Following are the list of some proteins:
1. BCL-2
2. BCL-XL
3. A1
4. BAX
Which of the protein(s) is/are NOT anti-apoptotic?
1. 2 and 4 only
2. 3 only
3. 4 only
4. 1 and 2 only
Correct Answer :-

4 only
Following are statements regarding terpene class of secondary metabolites in plants
1. Pyrethroids are monoterpene esters
2. Diterpenes are 20 carbon compounds
3. All terpenes are derived from the union of 4-carbon elements
4. Isopentenyl diphosphate and its isomer combine to form larger terpenes
Correct Answer :-

Isopentenyl diphosphate and its isomer combine to form larger terpenes

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Is there a difference between oncogenes and tumor suppressor genes?
1. No, oncogenes and tumor suppressor genes both stimulate the development of cancer, even in the absence of their becoming mutated.
2. Yes, oncogenes are genes that can cause cancer when they become mutated to become proto-oncogenes, whereas tumor suppressor genes play no role in cancer.
3. Yes, oncogenes prevent cancer from forming unless they are mutated to become proto-oncogenes, whereas tumor suppressor genes stimulate the formation of cancer even in the absence of mutation.
4. Yes, oncogenes are mutated versions of genes that promote abnormal cell division (such as ras and myc), whereas tumor suppressor genes normally hold cell division in check when it is not appropriate (such as Rb and p53).
Correct Answer :-
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Yes, oncogenes prevent cancer from forming unless they are mutated to become proto-oncogenes, whereas tumor suppressor genes stimulate the formation of cancer even in the absence of mutation.
In biosynthesis of cholesterol
1. 3-hydroxy-3 methyl glutaryl CoA (HMG CoA) is synthesized by mitochondrial HMGCoAsynthetase
2. Condensation of two farnesyl pyrophosphates to form squalene is freely reversible
3. HMG CoA reductase catalyzes the rate limiting step
4. The conversion of mevalonic acid to farnesyl pyrophosphate proceeds via condensation of three molecules of mevalonic acid
Correct Answer :-

3-hydroxy-3 methyl glutaryl CoA (HMG CoA) is synthesized by mitochondrial HMGCoAsynthetase
After hemorrhage, a subject develops hypovolemia and hypotension. Following are some of the statements regarding homeostatic measure taken by the body after hemorrhage. I. Increased release of vasopressin II. Increased water retention and reduced plasma osmolality III. Increased rate of afferent discharge from low pressure receptors of vascular system IV. Decreased rate of afferent discharge from high pressure receptors of vascular system Which one of the following is NOT correct in this condition?
1. I and II
2. Only III
3. Only I
4. II and IV
Correct Answer :-

Only I
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During the process of exocytosis, SNARES are needed for membrane to:
1. Induce Signaling
2. Flip Flop
3. Undergo lateral diffusion
4. Fuse
Correct Answer :-

Undergo lateral diffusion
In a reticulocyte lysate the polynucleotide 5'-AUGUUUUUUUUU directs the synthesis of Met-Phe-Phe-Phe. In the presence of farsomycin, a new antibiotic perfected by Fluhardy Pharmaceuticals, this polymer directs synthesis of Met-Phe only. From this information, which of the following deductions could you make about farsomycin?
1. It blocks translocation of peptidyl tRNA from the A-site to the P-site of the ribosome.
2. It prevents formation of the 80S initiation complex, which contains the initiator tRNA and both ribosomal subunits.
3. It inactivates peptidyl transferase activity on the large ribosomal unit.
4. It inhibits binding of aminoacyl – tRNA s to the A site in the ribosome
Correct Answer :-

It prevents formation of the 80S initiation complex, which contains the initiator tRNA and both ribosomal subunits.

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In an enzyme-catalyzed reaction, how does Vmax and Kmchange if the enzyme concentration is doubled while keeping all the reaction conditions unchanged, ensuring that substrate is not limiting?
1. Both Vmax and Km are doubled
2. Vmax is unchanged, while Km is doubled
3. Vmax is doubled, while Km is unchanged
4. Vmax is doubled, while Km is halved
Correct Answer :-
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Vmax is doubled, while Km is halved
Cell types are usually identified by using immuno-histochemistry technique. In such case, antibody has to be against:
1. Cell surface proteins
2. Ribosomal proteins
3. Cytosolic proteins
4. Mitochondrial proteins
Correct Answer :-

Mitochondrial proteins
In flies, frogs and chicks, gradients of morphogens determine the future antero-posterior (A-P) and dorso-ventral axes of the developing embryo. How is the A-P axis determined in C. elegans ?
1. Opposing gradients of chordin and BMP-4 establish the A-P axis.
2. Sperm entry leads to reorganization of the cytoskeleton and redistribution of maternally packaged PAR proteins, which in turn determine the A-P axis.
3. Bicoid protein is translated in the anterior of the fertilized egg leading to a gradient that determines the A-P axis
4. Beta-catenin becomes localized to the nucleus in the future anterior cells after fertilization.
Correct Answer :-

Bicoid protein is translated in the anterior of the fertilized egg leading to a gradient that determines the A-P axis
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Dye injected into an epithelial celll might be able to enter into an adjacent cell through a:
1. Desmosomes
2. Gap junction
3. Microtubule
4. Tight junction
Correct Answer :-

Microtubule
A student is performing site-directed mutagenesis in a gene that has been cloned in a vector plasmid. The total size of this plasmid is 8kb. The student had repeatedly tried to perform the mutagenesis experiment, however, there is no success. Which option of the following can be tried which will help in obtaining the mutants.
1. the size of the primers may be decreased
2. the size of the primers may be increased
3. the gene can be cloned into a larger size plasmid
4. the gene can be cloned into a much smaller size plasmid
Correct Answer :-

the gene can be cloned into a much smaller size plasmid

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A study was conducted to compare the proteome of normal individuals and cancer patients to identify new biomarkers for cancer. The study employed the use of 2D-DIGE by labeling the normal samples with Cy3 and cancer patient samples with Cy5. The Cy2 labeling was also employed in the experiment because:
1. There is no use of Cy2 labeling. The experiment could have been performed the same way with only two tags.
2. It will help in uniform labeling of the proteins from the cancer patient samples
3. Using three different colours will give rise to better analysis of the spots
4. It can act as an internal standard for normalization of all protein spots
Correct Answer :-
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It can act as an internal standard for normalization of all protein spots
"Heyflick's limit" refers to which one of the following phenomena?
1. Cellular senescence in vitro
2. RNA transport
3. DNA repair
4. Protein synthesis
Correct Answer :-

DNA repair
ß-lactoglobulin promoter is used for expression of gene in:
1. Liver
2. Mammary gland
3. Spleen
4. Lymph node
Correct Answer :-

Liver
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If association behaviour is to be evaluated for a aggregation prone protein by analytical ultracentrifugation, which of the following is true?
1. Sedimentation coefficient will decrease with increasing concentration
2. Sedimentation coefficient will increase with decreasing concentration
3. Sedimentation coefficient will increase with increasing concentration
4. Sedimentation coefficient will remain unchanged with increasing concentration
Correct Answer :-

Sedimentation coefficient will increase with increasing concentration
A mature human RBC:
1. Does not divide
2. Divides under stressful condition
3. Divides once a day
4. Divides every 120 days
Correct Answer :-

Divides once a day

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Catabolism of which of the following amino acids requires the direct involvement of O2?
1. Histidine
2. Glutamine
3. Isoleucine
4. Phenylalanine
Correct Answer :-
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Histidine
Anti-cancer vitamin is:
1. Pyridoxine
2. Phylloquinone
3. Retinol
4. Thiamine
Correct Answer :-

Retinol
Contrast the process of involution, epiboly and convergent extension.
1. Involution is movement of cells toward an axis to extend that axis, epiboly is flattening and spreading of epithelial cells to increase the amount of surface they cover and convergent extension is the movement of cells inside the embryo as a coherent she
2. Involution is movement of cells inside the embryo as a coherent sheet, epiboly is a flattening and spreading epithelial cells to increase the amount of surface they cover and convergent extension is movement of cells toward an axis to extend that axis.
3. Involution is movement of cells inside the embryo as a coherent sheet, epiboly is movement of cells toward an axis to extend that axis and convergent extension is a flattening and spreading epithelial cells to increase the amount of surface they cover
4. Involution is flattening of epithelial cells to increase the amount of surface they cover, epiboly is the movement of cells inside the embryo as a coherent sheet and convergent extension is movement of cells toward an axis to extend that axis.
Correct Answer :-

Involution is movement of cells toward an axis to extend that axis, epiboly is flattening and spreading of epithelial cells to increase the amount of surface they cover and convergent extension is the movement of cells inside the embryo as a coherent she
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End labeling of a DNA fragment is a prerequisite for Maxam Gilbert method of DNA sequencing. Which of the following enzymes is used to accomplish this?
1. Restriction endonucleases
2. Polynucleotide kinase
3. DNA ligase
4. Taq polymerase
Correct Answer :-

DNA ligase
Fluorescence microscopy is based on the ability of certain molecules to:
1. Absorb light of a given wavelength and then emit light of a shorter wavelength
2. Absorb light of a given wavelength and then emit light of a longer wavelength
3. Absorb light of many different wavelengths
4. Continuously emit light of a constant wavelength
Correct Answer :-

Continuously emit light of a constant wavelength

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A Bronsted acid becomes upon losing a proton
1. its conjugate acid
2. its conjugate base
3. highly reactive
4. a hydronium ion
Correct Answer :-
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its conjugate acid
In eukaryotic replication, priming of DNA synthesis and removal of RNA primer is catalysed by:
1. DNA Pol 8 and FEN1, respectively.
2. DNA Pol ? and PCNA, respectively.
3. DNA Pol a and PCNA, respectively.
4. DNA Pol a and FEN1, respectively.
Correct Answer:

DNA Pol a and PCNA, respectively.
To assess the impact of a newly identified drug when added to a culture of sub-confluent HeLa cells, a researcher analyzes the fluorescence activated cell sorting (FACS) profile of untreated (-Drug) cells versustreated (+ Drug) cells. Based on the FACS profile shown above, this drug inhibits
1. S phase of the cell cycle
2. G2/M phase of the cell cycle
3. G1 phase of the cell cycle
4. G0 phase of cell cycle
Correct Answer :-

G1 phase of the cell cycle
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In a mitochondrial respiration experiment, a researcher observed the following profile of oxygen consumption upon addition of the following compounds at times I, II and III.
1. ADP + Pi
2. Dinitrophenol, an uncoupler
3. Oligomycin, an ATPase inhibitor
4. Cyanide
5. Succinate
Which of the following describes the profile appropriately?
1. I-b; II - d; III – e
2. I-a; II - d; III – c
3. I-a; II - c; III – b
4. I-a; II - e; III – c
Correct Answer :-

I - b; II - d; III – e
For a FRET experiment, an oligonucleotide was labeled with Cy3 dye, which absorbs at 540 nm and fluoresces at 590 nm. Another oligonucleotide was labeled with Cy5 dye, which absorbs at 590 nm and emits fluorescence at 680 nm. Which of the following observations indicate a successful FRET experiment, whereby the two oligonucleotides anneal?
1. Excitation at 540 nm results in fluorescence emission at 680 nm
2. Excitation at 540 nm results in fluorescence emission at 590 nm
3. Excitation at 540 nm results in no fluorescence emission
4. Excitation at 590 nm results in fluorescence emission at 680 nm
Correct Answer :-

Excitation at 540 nm results in fluorescence emission at 590 nm

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What are the lin-4 and lin-14 genes of C. elegans ?
1. lin-4 and lin-14 are genes that are named for their control of the first division, and hence the lineage of the AB and P1 cells
2. lin-4 and lin-14 both encode miRNAs that serve as cell-cell signaling molecules in c. elegans
3. lin-4 and lin-14 are C. elegans versions of the HOX genes
4. lin-4 encodes a miRNA that represses lin-14 translation, which in turn regulates the timing of larval development
Correct Answer :-
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lin-4encodes a miRNA that represses lin-14 translation, which in turn regulates the timing of larval development
What is the isoelectric point (pI) of the amino acid lysine with pKa1= 2.2, pKa2= 9.0 and pKa3= 10.8.
1. 7.3
2. 5.6
3. 9.9
4. 9
Correct Answer :-

7.3
What would happen to red blood cells if the haem group were removed from haemoglobin?
1. White blood cells would not be able to reproduce.
2. Platelets would not be able to reproduce, For
3. Red blood cells would not be able to transport oxygen
4. Blood clot formation would be inhibited.
Correct Answer :-

Red blood cells would not be able to transport oxygen
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Which of the following changes in the gene encoding a lysosomal protein is not expected to impair the translocation of the protein across the ER membrane?
1. The conversion of the two leucine codons and one phenylalanine codon near the translation initiation codon to an isoleucine codon, an aspartic acid codon and a glutamic acid codon, respectively
2. The conversion of the sole lysine codon after the translation initiation codon to an arginine codon
3. The conversion of the sole lysine codon after the translation initiation codon to an aspartic acid codon
4. The deletion of the nucleotide specifying the 25 amino acids following the translation initiation codon
Correct Answer :-

The deletion of the nucleotide specifying the 25 amino acids following the translation initiation codon
Competitive inhibition is overcome by adding substrate shows that:
1. enzymes are pH dependent
2. enzymes are specific in nature
3. enzymes are biocatalysts
4. enzymes are made of proteins
Correct Answer :-

enzymes are biocatalysts

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The technique known as two-hybrid analysis for detecting interacting gene products depend on
1. Direct binding of a Gal4p activation domain to a DNA sequence in the promoter region.
2. Activation of DNA polymerase by the nearby binding of hybridizing protein complexes.
3. Stimulation of transcription by interaction of two Gal4p domains via fused protein sequences.
4. Having a promoter that responds directly to one of the two proteins whose interactions is beingmeasured.
Correct Answer :-
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Activation of DNA polymerase by the nearby binding of hybridizing protein complexes.
The enzyme of E.coli is a nuclease that initiates the repair of double stranded DNA breaks by homologous recombination
1. RNA polymerase
2. DNA glycosylase
3. DNA ligase
4. DNA polymerase
Correct Answer :-

DNA glycosylase
The putative interaction between two proteins was identified by yeast two hybrid assays. Which of the following technique cannot be employed to further confirm the interaction?
1. Electrophoretic Mobility Shift Assay
2. Fluorescence Resonance Energy Transfer analysis
3. Protein Microarray
4. Pull Down Assay
Correct Answer :-

Pull Down Assay
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The molecule which acts directly on an enzyme to lower its catalytic rate is
1. regulator
2. repressor
3. inhibitor
4. modulator
Correct Answer :-

repressor
The pluripotency of the inner cell mass in mammals is maintained by a core of three transcription factors namely
1. Oct 4, Cdx 2 and Nanog
2. Oct 4, Sox 2 and Cdx 2
3. Oct 4, Sox 2 and Nanog
4. Sox 2, Nanog and Cdx 2
Correct Answer :-

Oct 4, Sox 2 and Nanog

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The precursor of all N-linked oligosaccharides contains:
1. Two glucose, nine mannose and three N-acetylglucos

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This post was last modified on 19 June 2020