Subject Code:2170401
GUJARAT TECHNOLOGICAL UNIVERSITY
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BE - SEMESTER-VII(NEW) EXAMINATION - SUMMER 2019
Subject Name:Enzymes and Proteins
Time:02:30 PM TO 05:00 PM
Date:10/05/2019
Total Marks: 70
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Instructions:
- Attempt all questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
Q.1 What is molten globule?
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(a) Discuss in brief the forces that stabilise protein structure.
(b) Explain allosteric regulation of enzyme action with example.
Q.2 What are the folding funnels?
(a) Discuss secondary structural elements of protein.
(b) Define enzyme, cofactor, apoenzyme and holoenzyme and describe different classes of enzymes.
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OR
(a) Describe purification of crude enzyme extracts from microbial sources.
(b) Explain Ramachandran map.
(c) Write the factors that affect globin folding patterns.
Q.3 Enlist various methods of purification of enzymes. Discuss any one in detail
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OR
(a) Enlist important enzymes in food industry and discuss the importance of any one.
(b) Explain principle of affinity chromatography.
(c) Write the Benefits and limitation of enzyme immobilisation.
Q.4 Explain multi-subunit proteins
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(a) Explain protein-DNA interactions with example.
(b) How pH and temperature affects enzyme activity? Comment on the deactivation kinetics.
OR
(a) Explain the principle of thin layer chromatography.
(b) Discuss any protein misfiling disease.
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(c) Explain the principle and procedure of affinity chromatography for protein purification.
Q.5 What is proteostasis?
(a) Explain thermodynamics of protein folding.
(b) Give the derivation of Michelis-Menten equation and comment on the significance of it.
OR
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(a) What is levinthal paradox?
(b) Explain general properties of protein protein interfaces.
(c) Discuss in detail GroEL-GroES mechanism for protein folding.
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