Download GTU BE/B.Tech 2019 Summer 7th Sem New 2170401 Enzymes And Proteins Question Paper

Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Summer 7th Sem New 2170401 Enzymes And Proteins Previous Question Paper

1
Seat No.: ________ Enrolment No.___________

GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER ?VII(NEW) EXAMINATION ? SUMMER 2019
Subject Code:2170401 Date:10/05/2019

Subject Name:Enzymes and Proteins

Time:02:30 PM TO 05:00 PM Total Marks: 70

Instructions:

1. Attempt all questions.

2. Make suitable assumptions wherever necessary.

3. Figures to the right indicate full marks.
*************

MARKS

Q.1 (a) What is molten globule? 3
(b) Discuss in brief the forces that stabilise protein structure. 4
(c) Explain allosteric regulation of enzyme action with example. 7
Q.2 (a) What are the folding funnels? 3
(b) Discuss secondary structural elements of protein. 4
(c)
Define enzyme, cofactor, apoenzyme and holoenzyme and describe different
classes of enzymes.
7
OR
(c) Describe purification of crude enzyme extracts from microbial sources. 7
Q.3 (a) Explain Ramachandran map. 3
(b) Write the factors that affect globin folding patterns. 4
(c) Enlist various methods of purification of enzymes. Discuss any one in detail 7
OR
Q.3 (a)
Enlist important enzymes in food industry and discuss the importance of any
one.
3
(b) Explain principle of affinity chromatography. 4
(c) Write the Benefits and limitation of enzyme immobilisation. 7
Q.4 (a) Explain multi-subunit proteins 3
(b) Explain protein-DNA interactions with example. 4
(c)
How pH and temperature affects enzyme activity? Comment on the
deactivation kinetics.
7
OR
Q.4 (a) Explain the principle of thin layer chromatography. 3
(b) Discuss any protein misfiling disease. 4
(c)
Explain the principle and procedure of affinity chromatography for protein
purification.
7
Q.5 (a) What is proteostasis? 3
(b) Explain thermodynamics of protein folding. 4
(c)
Give the derivation of Michelis-Menten equation and comment on the
significance of it.
7
OR
Q.5 (a) What is levinthal paradox? 3
(b) Explain general properties of protein protein interfaces. 4
(c) Discuss in detail GroEL-GroES mechanism for protein folding. 7
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