Rajiv Gandhi University of Health Sciences
First Year M.Pharm Degree Examination – Nov 2011
[Time: 3 Hours]
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BIOPROCESS TECHNOLOGY
(RS 2 & RS 3)
Q.P. CODE: 9313
Your answers should be specific to the questions asked
Draw neat labeled diagrams wherever necessary
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[Max. Marks: 100]
LONG ESSAY (Answer any FOUR) 4 X 25 = 100 Marks
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Write notes on:
a) Air Spargers
b) Achievement and maintenance of aseptic conditions (12 + 13 = 25 )
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a) Distinguish between:
i) aerobic and anaerobic fermentations
ii) ion exchange and affinity chromatography
iii) Single and 2-stage continuous fermentation systems.
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b) Discuss economic considerations of a fermentation process (15 + 10 = 25 )
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a) Write a note on agitation in fermentations
b) Discuss the importance of various probes in a fermenter
c) How is foam controlled? (10+10+5 = 25)
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Write short notes on:
a) Metabolic response assay
b) Cell disintegration methods
c) Enzyme inhibition
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d) Standards of air purity
e) impeller design (5x5 = 25)
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a) Explain the Rheology of fermentation system
b) Discuss Principles and application of solvent extraction and electrophoresis. (12+13 = 25)
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a) Discuss fed-batch fermentations. How are they different from continuous fermentation? (12+13 = 25)
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