Download PTU B.Tech 2020 March Biotechnology 3rd Sem BTBT 302 Foundations Of Biotechnology Question Paper

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Roll No. ‘ ‘ ‘ ‘ ‘ ‘ ‘ ‘ ‘ ‘ ‘ Total No. of Pages : 02

Total No. of Questions : 09

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B.Tech. (BT) (2012 to 2017) (Sem.-3)

FOUNDATIONS OF BIOTECHNOLOGY

Subject Code : BTBT-302

M.Code : 55072

Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

SECTION-A

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1. Answer briefly :
   1. How is Meiosis different from Mitosis?
   2. Which technique would you use for separating sub-cellular organelle from cell lysate of a eukaryotic organism and why?
   3. What is electrophoresis?
   4. Define Monoclonal Antibodies (Mab’s). How do they provide protection?
   5. What is the difference between Modern and Old Biotechnology?

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   6. What is a nucleosome?
   7. Define an Antibiotic. Which antibiotic was first discovered from a microorganism?
   8. What is bioremediation? Explain giving a suitable example.
   9. What are biopharmaceuticals? How are they produced? Cite an example.
   10. Give the organization of Nucleosome.

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SECTION-B

1. What is the principle and applications of UV-Visible spectroscopy?
2. Explain the structural features of Watson and Crick Model of DNA.

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3. Discuss the role of microorganisms in treatment of Sewage.
4. Write short notes on any two (approx. 600 words each) :
   1. Biopesticides
   2. Gene Therapy
   3. Biofertilizers
   4. Bioengineered Food.

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5. Briefly discuss the technique of Bio-augmentation by citing a relevant case.

SECTION-C

1. Discuss the role of bacteria and fungi in bioremediation.
2. Describe the production of biofertilizers based on Rhizobium and also explain its significance in Agriculture.

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3. Give an account of column chromatography and add a note on Reverse phase chromatography.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.

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