HNBUMU PH.D Entrance Exam 2020 Subject Wise Questions With Solutions (H.N.B. Uttarakhand Medical Education University)
Subject Title: Chemistry of Nucleic acids And Biochemical Techniques
Year: B.Sc Semester: II Updated on: 22-3-2024
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Unit - I: Composition Of Nucleic Acids
Unit - II:
Essay Questions :
- Define Phosphodiester bond? Write about the factors affecting the stability of phophodiester bond? (effect of acids,Bases,Enzymes)
- Explain in detail about the Photochemical &spectral characteristics of Nucleic acids?
Short Answer:
- Functions of Nucleotides
- Structures of Purines & Pyrimidines
- Differences between DNA & RNA
- Structure of Nucleic acids
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Essay Questions :
- Write in detail about the structure of DNA proposed by Watson & crick
- Define Renaturation ? Explain about the Reassociation Kinetics of DNA
- Define Cot? Explain about the Cot Half, Cot Curves & their Significance
- Explain different types of RNA &their Functions
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Short Answers
- Denaturation of Nucleic Acids, Tm Values
- Super Coiling of DNA
- Hypo chromic Effect
- Renaturation of DNA
- Define Tm value & its Significance
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Unit - III: Spectrophotometric and Centrifugation Technique
Essay Questions
- Describe the principle, instrumentation & applications of Spectrometer?
- Write about the principle &application Centrifugation Technique?
- Write in detail about the Differential Centrifugaton?
- Explain the principle & instrumentation of Ultracentrifugation?
Short Answers:
- Limitations of Beer-Lamberts Law
- Principle of Fluorimetry
- Molar Extinction Coefficient
- Beer-Lamberts law
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Unit - IV: Chromatography Techniques
Essay Questions
- Write about the principle applications of Affinity chromatography?
- Write about the principle applications of Gel filtration chromatography?
- Write about the principle applications of ion exchange chromatography?
- Write about the principle applications of Paper chromatography?
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ShortAnswers
- Thin layer chromatography
- Applications of Paper Chromatography
- Cation Exchanger
- Anion Exchanger
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