Subject Title: Microbiology and r-DNA Technology
Year: Semester: VI Semester
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Unit - I: Microbiology
Essay Questions:
- Write about the industrial uses of Aspergillus niger and Spirulina?
- Write about the industrial uses of Aspergillus niger and Yeast?
- Explain in detail about the structure and composition of viruses?
- Write about the lytic cycle and Lysogenic cycle of Lambda phage?
- Write about the Bacterial growth curve?
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Short Answer:
- Sporulation
- Types of Media
- Motility of Bacteria
- Gram Staining
- Synchronous Growth
- Differences between the Eukaryotes and Prokaryotes
- TMV
- HIV
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Unit - II: r-DNA technology
Essay Questions:
- Write in detail about the Cloning Strategies?
- Define cloning vectors? Explain about the plasmid vectors with neat diagram
- Write about the various tools used in r-DNA technology?
- Define Molecular marker? Write in detail about the RFLP?
- Explain DNA sequencing by using Maxam Gilbert and Sanger’s Method?
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Short Answers
- Restriction Mapping
- Host
- Restriction endonucleases
- AFLP
- RAPD
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Unit - III: r-DNA technology II
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Essay Questions
- Write about the construction of c-DNA libraries & its limitations?
- Explain the principle and applications of PCR?
- Write in detail about the southern blotting technique & its applications?
- Write about the production of insulin and Human growth hormone?
- Explain the importance of edible vaccines?
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Short Questions
- Bt cotton
- Edible vaccines
- Western blotting
- Northern blotting
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