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Subject Title: Tissue Culture and Biotechnology
Year: II
Semester: VI
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Prepared by: K Gouthami
Updated on: 23.03.
Unit - I: Tissue Culture
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Essay Questions:
- Write in detail about the technical procedures employed in plant tissue culture.
- Write an essay on Anther and Pollen Culture.
- Write in detail about Organ Culture.
- Write in detail about Single Cell Culture.
- Write an essay on protoplast culture and callus culture.
- Define somatic hybridization? Describe different steps involved in it and mention its uses.
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Short Answer Questions:
- Significance of plant tissue culture.
- Micro propagation.
- Sterilization Methods.
- Culture Medium.
- Major types of Culture Media.
- Embryo Culture.
- Root Culture.
- Shoot Tip Culture.
- Leaf Culture.
- Ovule and Ovary Culture.
- Callus Culture — Importance.
- Single Cell Culture — Importance.
- Protoplast Culture — Importance.
- Cybrids and Somatic hybrids.
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Unit - II: Tissue Culture and Biotechnology
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Essay Questions:
- Write in detail about the various applications of Tissue culture technology.
- Write an essay on hairy roots and their production of Secondary metabolites.
- Write an essay on Biotechnology and its applications in various fields.
- What is Gene Cloning? Write in detail about Gene Cloning?
- Write about the enzymes used in Gene Cloning.
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Short Answer Questions:
- Somaclonal variants.
- Artificial seeds.
- Secondary metabolites and Tissue culture.
- Disease free plants-thro’ Tissue culture.
- Tissue culture - Stress Resistant Plants.
- Ri - Plasmid.
- Applications of hairy root cultures.
- Hairy Roots and Secondary Metabolites.
- Restriction Endonucleases; Ligases and Polymerases.
- PCR mediated Gene Cloning.
- Differences between Normal and PCR Gene Cloning.
- Agricultural Biotechnology.
- Medical and Industrial Biotechnology
- Genetic Engineering.
- Gene Therapy and DNA finger printing.
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Unit - III: Tissue Culture and Biotechnology
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Essay Questions:
- Write in detail about all Gene Cloning Vectors / Vehicles.
- Write an essay on Genomic Libraries.
- What is PCR? Describe in detail about PCR and its applications.
- What is Gene transfer? Describe in detail about the Gene Transfer Methods in Plants.
- Describe in detail about the Physical Gene Transfer Methods.
- Write in detail about the production and applications of transgenic plants in crop improvement.
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Short Answer Questions:
- Plasmids and Cosmids.
- Phage Vectors / Bacteriophages.
- Phagemids.
- YAC.
- PAC.
- pUC Plasmids.
- cDNA and Genomic Library — Uses.
- cDNA and Genomic Library — Differences.
- DNA Libraries — Screening.
- Colony Hybridization.
- Describe the role of Rdna technology through its applications in the field of Medicine and other fields.
- Write about the Microprojectile method of gene transfer.
- Agrobacterium — Gene Transfer.
- Direct and Indirect Gene Transfer.
- Types of transgenic plants.
- Bt-Cotton and Bt-Brinjal.
- Applications of transgenic plants.
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