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Delhi University Entrance Test (DUET) 2020 Previous Year Question Paper With Answer Key

DU PhD in Plant Molecular Biology N Biotech
Topic: PMBB PHD S2
1) In order to align very distantly related protein sequences, one should use
[Question ID = 2356]
1. higher number PAM scoring matrix [Option ID = 9418]
2. lower number PAM scoring matrix [Option ID = 9419]
3. higher number BLOSUM scoring matrix [Option ID = 9420]
4. higher gap penalty [Option ID = 9421]
Correct Answer :
higher number PAM scoring matrix [Option ID = 9418]
2) Which of the following file formats can be used to store genome annotations (e.g. gene coordinates)?
[Question ID = 2357]
1. FASTA [Option ID = 9422]
2. FASTQ [Option ID = 9423]
3. GFF [Option ID = 9424]
4. PDB [Option ID = 9425]
Correct Answer :
GFF [Option ID = 9424]
3) Which of the following techniques can be used to identify DNA sequences where a particular transcription factor binds?
[Question ID = 2358]
1. ChIP analysis [Option ID = 9426]
2. RNAseq analysis [Option ID = 9427]
3. Western analysis [Option ID = 9428]
4. RNase protection analysis [Option ID = 9429]
Correct Answer :
ChIP analysis [Option ID = 9426]
4) Which of the following is NOT a genome editing technique?
[Question ID = 2359]
1. Transcription activatorlike effector nucleases (TALENs) system [Option ID = 9430]
2. CRISPRCas system [Option ID = 9431]
3. Zinc finger nuclease (ZFNs) system [Option ID = 9432]
4. Gateway cloning system [Option ID = 9433]
Correct Answer :
Gateway cloning system [Option ID = 9433]
5) `RAvr interactions' are important in determining resistance of plants against
[Question ID = 2360]
1. Submergence stress [Option ID = 9434]
2. Low temperature stress [Option ID = 9435]
3. Excess Na stress
+
[Option ID = 9436]
4. Pathogen stress [Option ID = 9437]
Correct Answer :
Pathogen stress [Option ID = 9437]
6) M. Chalfie, S. Osamu and R.Y. Tsien received Noble Prize for the discovery of one of the following:
[Question ID = 2361]
1. Green fluorescent protein [Option ID = 9438]
2. Restriction endonucleases [Option ID = 9439]
3. DNA Helicases [Option ID = 9440]
4. DNA Ligases [Option ID = 9441]
Correct Answer :
Green fluorescent protein [Option ID = 9438]
7) A genomic library is:
[Question ID = 2362]
1. a database where the sequence of an organism's genome is stored. [Option ID = 9442]
2. a collection of clones with different DNA fragments representing genomic DNA of an organism. [Option ID = 9443]
3. a book that describes how to isolate DNA from an organism. [Option ID = 9444]
4. a place where the information of the genetic organization of organisms is stored. [Option ID = 9445]

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Correct Answer :
a collection of clones with different DNA fragments representing genomic DNA of an organism. [Option ID = 9443]
8) The role of restriction endonucleases in bacterial cells is to:
[Question ID = 2363]
1. degrade the bacterial chromosome into small pieces during replication [Option ID = 9446]
2. degrade the invading phage DNA [Option ID = 9447]
3. produce RNA primers for replication [Option ID = 9448]
4. aid the transcription process [Option ID = 9449]
Correct Answer :
degrade the invading phage DNA [Option ID = 9447]
9) Poly A tail is added to the transcript by
[Question ID = 2364]
1. DNA polymerase using DNA as a template [Option ID = 9450]
2. poly A polymerase using DNA as a template [Option ID = 9451]
3. RNA polymerase posttranscriptionally [Option ID = 9452]
4. poly A polymerase posttranscriptionally [Option ID = 9453]
Correct Answer :
poly A polymerase posttranscriptionally [Option ID = 9453]
10) Retrotransposons require the following for retrotransposition
[Question ID = 2365]
1. DNA replication
[Option ID = 9454]
2. reverse transcription
[Option ID = 9455]
3. genome editing
[Option ID = 9456]
4. cutandpaste of DNA
[Option ID = 9457]
Correct Answer :
reverse transcription
[Option ID = 9455]
11) Transcriptional elongation involves
[Question ID = 2366]
1. phosphorylation of RNA polymerase II [Option ID = 9458]
2. methylation of 5'end of RNA [Option ID = 9459]
3. removal of first intron [Option ID = 9460]
4. removal of 5' UTR [Option ID = 9461]
Correct Answer :
phosphorylation of RNA polymerase II [Option ID = 9458]
12) Protooncogenes are
[Question ID = 2367]
1. normal cellular genes [Option ID = 9462]
2. cancer promoting genes [Option ID = 9463]
3. tumor suppressor genes [Option ID = 9464]
4. portable oncogenes [Option ID = 9465]
Correct Answer :
normal cellular genes [Option ID = 9462]
13) Mutagenesis by ethidium bromide is brought about by
[Question ID = 2368]
1. incorrect basepairing [Option ID = 9466]
2. indels following insertion [Option ID = 9467]
3. instability of basepairing [Option ID = 9468]
4. translesion synthesis during replication [Option ID = 9469]
Correct Answer :
translesion synthesis during replication [Option ID = 9469]
14) Which step of translation does not require GTP hydrolysis?
[Question ID = 2369]

---

1. Peptide bond formation [Option ID = 9470]
2. Translocation of ribosomes [Option ID = 9471]
3. Assembly of ribosome subunits at the ShineDalgarno sequence [Option ID = 9472]
4. Dissociation of ribosome subunits at stop codon [Option ID = 9473]
Correct Answer :
Peptide bond formation [Option ID = 9470]
15) A polymer, which is deposited as an early response to pathogen attack in plants is one of the following:
[Question ID = 2370]
1. Stachyose [Option ID = 9474]
2. Cellulose [Option ID = 9475]
3. Xylose [Option ID = 9476]
4. Callose [Option ID = 9477]
Correct Answer :
Callose [Option ID = 9477]
16) Which of the following is NOT a PathogenesisRelated (PR) protein?
[Question ID = 2371]
1. 1,3glucanase [Option ID = 9478]
2. Proteinase inhibitor [Option ID = 9479]
3. Polyubiquitin [Option ID = 9480]
4. Chitinase [Option ID = 9481]
Correct Answer :
Polyubiquitin [Option ID = 9480]
17) Which of the following is an intermediate for the biosynthesis of terpenes?
[Question ID = 2372]
1. Norepinephrine [Option ID = 9482]
2. Phenylalanine [Option ID = 9483]
3. Coumaric acid [Option ID = 9484]
4. Mevalonic acid [Option ID = 9485]
Correct Answer :
Mevalonic acid [Option ID = 9485]
18) Which of the following class of compounds is a natural feeding deterrent against herbivores in plants?
[Question ID = 2373]
1. Sterols [Option ID = 9486]
2. Pyrethroids [Option ID = 9487]
3. Defensins [Option ID = 9488]
4. Carotenoids [Option ID = 9489]
Correct Answer :
Pyrethroids [Option ID = 9487]
19) Which of the following is a plant antimicrobial protein?
[Question ID = 2374]
1. Leghaemoglobin [Option ID = 9490]
2. Thaumatin [Option ID = 9491]
3. Trypsin [Option ID = 9492]
4. Isothiocyanate [Option ID = 9493]
Correct Answer :
Thaumatin [Option ID = 9491]
20) Which of the following is an example of a plant disease resistance gene?
[Question ID = 2375]
1. virA
[Option ID = 9494]
2. nifA
[Option ID = 9495]
3. Xa21
[Option ID = 9496]
4. nos
[Option ID = 9497]
Correct Answer :
Xa21

---

[Option ID = 9496]
21) Which of the following is an important component of the signaling pathway during Systemic Acquired Resistance (SAR)?
[Question ID = 2376]
1. Chitin [Option ID = 9498]
2. Glucose [Option ID = 9499]
3. Maleic acid [Option ID = 9500]
4. Salicylic acid [Option ID = 9501]
Correct Answer :
Salicylic acid [Option ID = 9501]
22) Which of the following pairs is haploid in nature?
[Question ID = 2377]
1. Nucellus and antipodal cells [Option ID = 9502]
2. Antipodal cells and egg cell [Option ID = 9503]
3. Antipodal cells and megaspore mother cell [Option ID = 9504]
4. Nucellus and primary endosperm nucleus [Option ID = 9505]
Correct Answer :
Antipodal cells and egg cell [Option ID = 9503]
23) Endosperm is formed during double fertilization by
[Question ID = 2378]
1. an ovum and the male gamete
[Option ID = 9506]
2. one polar nuclei and one male gamete
[Option ID = 9507]
3. two polar nuclei and one male gamete
[Option ID = 9508]
4. two polar nuclei and two male gametes
[Option ID = 9509]
Correct Answer :
two polar nuclei and one male gamete
[Option ID = 9508]
24) Lateral roots originate from the
[Question ID = 2379]
1. epiblema [Option ID = 9510]
2. cortical cells [Option ID = 9511]
3. endoderm cells [Option ID = 9512]
4. pericycle cells [Option ID = 9513]
Correct Answer :
pericycle cells [Option ID = 9513]
25) Long filamentous threads protruding at the end of a young cob of maize are
[Question ID = 2380]
1. anthers [Option ID = 9514]
2. styles [Option ID = 9515]
3. ovaries [Option ID = 9516]
4. hairs [Option ID = 9517]
Correct Answer :
styles [Option ID = 9515]
26) Which of the following photoreceptors has homology with DNA photolyases?
[Question ID = 2381]
1. Phototropins [Option ID = 9518]
2. Cryptochromes [Option ID = 9519]
3. Phytochromes [Option ID = 9520]
4. UVR8 [Option ID = 9521]
Correct Answer :
Cryptochromes [Option ID = 9519]
27) `Florigen', the mobile signal involved in transition to flowering in plants is a
[Question ID = 2382]
1. hormone [Option ID = 9522]
2. nucleic acid [Option ID = 9523]

---

3. protein [Option ID = 9524]
4. carbohydrate [Option ID = 9525]
Correct Answer :
protein [Option ID = 9524]
28) Which of the following plant hormones employs a twocomponent sensorregulator system to regulate gene expression?
[Question ID = 2383]
1. Brassinosteroid [Option ID = 9526]
2. Auxin [Option ID = 9527]
3. Cytokinin [Option ID = 9528]
4. Abscisic acid [Option ID = 9529]
Correct Answer :
Cytokinin [Option ID = 9528]
29) The receptor of which of the following hormones is a component of E3 ligase involved in ubiquitinmediated protein
degradation?
[Question ID = 2384]
1. Cytokinin [Option ID = 9530]
2. Auxin [Option ID = 9531]
3. Ethylene [Option ID = 9532]
4. Gibberellin [Option ID = 9533]
Correct Answer :
Auxin [Option ID = 9531]
30) The production of which of the following hormones is triggered during invasion of plants by necrotrophs?
[Question ID = 2385]
1. Salicylic acid [Option ID = 9534]
2. Jasmonic acid [Option ID = 9535]
3. Ethylene [Option ID = 9536]
4. Nitric oxide [Option ID = 9537]
Correct Answer :
Jasmonic acid [Option ID = 9535]
31) The receptor for brassinosteroid, the steroid hormone present in plants, is localized in the
[Question ID = 2386]
1. cytoplasm [Option ID = 9538]
2. nucleus [Option ID = 9539]
3. plasma membrane [Option ID = 9540]
4. cell wall [Option ID = 9541]
Correct Answer :
plasma membrane [Option ID = 9540]
32) Which of the following proteins is NOT in the same superfamily having seven closely packed transmembrane helices as
the other three?
[Question ID = 2387]
1. Bacteriorhodopsin [Option ID = 9542]
2. Channelrhodopsin [Option ID = 9543]
3. Gproteincoupled receptor [Option ID = 9544]
4. Aquaporin [Option ID = 9545]
Correct Answer :
Aquaporin [Option ID = 9545]
33) Which of the following is NOT a common second messenger in cell signaling?
[Question ID = 2388]
1. Ca
2+ [Option ID = 9546]
2. Cyclic adenosine monophosphate [Option ID = 9547]
3. Diacylglycerol [Option ID = 9548]
4. Tyrosine [Option ID = 9549]
Correct Answer :
Tyrosine [Option ID = 9549]
34) Which of the following events normally activates a GTPbinding protein?
[Question ID = 2389]
1. GTP hydrolysis by the protein [Option ID = 9550]
2. Activation of an upstream GTPaseactivating protein [Option ID = 9551]
3. Activation of an upstream guanine nucleotide exchange factor [Option ID = 9552]
4. Phosphorylation of a bound GDP by an upstream phosphorylase [Option ID = 9553]

---

Correct Answer :
Activation of an upstream guanine nucleotide exchange factor [Option ID = 9552]
35) Consider visual transduction in rod photoreceptors in the vertebrate retina. Which of the following steps does NOT
normally amplify the signal in this pathway?
[Question ID = 2390]
1. Activation of transducin by active rhodopsin [Option ID = 9554]
2. Blockage of Na + influx by cationchannel closure [Option ID = 9555]
3. Cationchannel closure due to cGMP depletion [Option ID = 9556]
4. Depletion of cGMP by active cGMP phosphodiesterase [Option ID = 9557]
Correct Answer :
Cationchannel closure due to cGMP depletion [Option ID = 9556]
36) Gibberellic acid (GA) signaling initiates by binding of GA to its receptor
[Question ID = 2391]
1. DELLA [Option ID = 9558]
2. GID1 [Option ID = 9559]
3. GA oxidase [Option ID = 9560]
4. PIF [Option ID = 9561]
Correct Answer :
GID1 [Option ID = 9559]
37) What would you need to know to determine quantum yield of photosynthesis accurately?
[Question ID = 2392]
1. Amount of CO fixed and O
2
released
2
[Option ID = 9562]
2. Amount of CO fixed and light absorbed
2
[Option ID = 9563]
3. Amount of starch synthesized [Option ID = 9564]
4. Amount of 3phosphoglycerate synthesized [Option ID = 9565]
Correct Answer :
Amount of CO fixed and light absorbed
2
[Option ID = 9563]
38) The statistical test to determine `goodness of fit' is:
[Question ID = 2393]
1. ttest [Option ID = 9566]
2. Chisquare test [Option ID = 9567]
3. ztest [Option ID = 9568]
4. ftest [Option ID = 9569]
Correct Answer :
Chisquare test [Option ID = 9567]
39) Expression levels of a gene can be monitored using following two techniques:
[Question ID = 2394]
1. Southern hybridization, quantitative RTPCR [Option ID = 9570]
2. Quantitative RTPCR, nuclear runon assay [Option ID = 9571]
3. Southern hybridization, nuclear runon assay [Option ID = 9572]
4. Quantitative PCR and DNase footprinting assay [Option ID = 9573]
Correct Answer :
Quantitative RTPCR, nuclear runon assay [Option ID = 9571]
40) Neoisoschizomers are the restriction endonucleases that have
[Question ID = 2395]
1. different recognition and cleavage sites [Option ID = 9574]
2. different recognition and similar cleavage sites [Option ID = 9575]
3. same recognition and different cleavage sites [Option ID = 9576]
4. same recognition and cleavage sites [Option ID = 9577]
Correct Answer :
same recognition and different cleavage sites [Option ID = 9576]
41) Homopolymer tailing of cDNA can be achieved with one of the following:
[Question ID = 2396]
1. Klenow polymerase
[Option ID = 9578]
2. Terminal deoxynucleotidyl transferase
[Option ID = 9579]
3. T4 DNA ligase

---

[Option ID = 9580]
4. Taq DNA polymerase
[Option ID = 9581]
Correct Answer :
Terminal deoxynucleotidyl transferase
[Option ID = 9579]
42) Which of the following factors does not influence electrophoretic mobility?
[Question ID = 2397]
1. Molecular weight [Option ID = 9582]
2. Shape of molecule [Option ID = 9583]
3. Size of molecule [Option ID = 9584]
4. Stereochemistry of molecule [Option ID = 9585]
Correct Answer :
Stereochemistry of molecule [Option ID = 9585]
43) Function of Betamercaptoethanol in SDSpage is
[Question ID = 2398]
1. to give negative charge to amino acids in the proteins [Option ID = 9586]
2. for oxidation of disulfide bonds in the proteins [Option ID = 9587]
3. for reduction of disulfide bonds in the proteins [Option ID = 9588]
4. for breaking hydrogen bonds in the proteins [Option ID = 9589]
Correct Answer :
for reduction of disulfide bonds in the proteins [Option ID = 9588]
44) Mass spectrometer separates ions on the basis of which of the following?
[Question ID = 2399]
1. Mass [Option ID = 9590]
2. Charge [Option ID = 9591]
3. Molecular weight [Option ID = 9592]
4. Mass to charge ratio [Option ID = 9593]
Correct Answer :
Mass to charge ratio [Option ID = 9593]
45) Which of the following sequencing technologies is capable of delivering readlengths of more than 10 kb?
[Question ID = 2400]
1. ABI [Option ID = 9594]
2. Illunina [Option ID = 9595]
3. Pacbio [Option ID = 9596]
4. SOLiD [Option ID = 9597]
Correct Answer :
Pacbio [Option ID = 9596]
46) Which of the following approach is utilized by sequence alignment tool `BLAST' to search sequence databases?
[Question ID = 2401]
1. Global sequence alignment
[Option ID = 9598]
2. Pairwise sequence alignment
[Option ID = 9599]
3. Multiple sequence alignment
[Option ID = 9600]
4. All of these
[Option ID = 9601]
Correct Answer :
Pairwise sequence alignment
[Option ID = 9599]
47) In biochemical reactions, which of the following proteins can be used as a chaperone?
[Question ID = 2402]
1. transporters [Option ID = 9602]
2. heat shock proteins [Option ID = 9603]
3. ubiquitins [Option ID = 9604]
4. transcription factors [Option ID = 9605]

---

Correct Answer :
heat shock proteins [Option ID = 9603]
48) The selectable marker gene nptII encodes for a
[Question ID = 2403]
1. phosphotransferase
[Option ID = 9606]
2. kinase
[Option ID = 9607]
3. phosphatase
[Option ID = 9608]
4. methylase
[Option ID = 9609]
Correct Answer :
phosphotransferase
[Option ID = 9606]
49) In a biochemical reaction, which one can cleave proteins?
[Question ID = 2404]
1. Ligase [Option ID = 9610]
2. RecA [Option ID = 9611]
3. RecBCD [Option ID = 9612]
4. DNA polymerase [Option ID = 9613]
Correct Answer :
RecA [Option ID = 9611]
50) Which of the following plants is commonly used for production of bioethanol?
[Question ID = 2405]
1. Jatropha [Option ID = 9614]
2. Brassica [Option ID = 9615]
3. Sugarcane [Option ID = 9616]
4. Pongamia [Option ID = 9617]
Correct Answer :
Sugarcane [Option ID = 9616]

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