Download DUET Master 2018 DU PhD in Microbiology Question Paper With Answer Key

Download DUET (Delhi University Entrance Test conducted by the NTA) 2018 DU PhD in Microbiology Question Paper With Solution Key

This post was last modified on 29 January 2020

Download Document

DUET Last 10 Years 2011-2021 Question Papers With Answer Key || Delhi University Entrance Test conducted by the NTA

DU PhD in Microbiology

Topic:- DU_J18_PHD_MICRO

1) The yoghurt preparation requires the presence of 1:1 ratio of the following microorganisms:
--- Content provided by⁠ FirstRanker.com ---

[Question ID = 52175]
1. Streptococcus thermophilus and Lactobacillus bulgaricus [Option ID = 88692]
2. Streptococcus thermophilus and Lactococcus diacetilactis [Option ID = 88693]
3. Lactococcus lactis and Streptococcus thermophilus [Option ID = 88695]
4. Lactococcus diacetilactis and Lactobacillus bulgaricus [Option ID = 88694]
Correct Answer :-

Streptococcus thermophilus and Lactobacillus bulgaricus [Option ID = 88692]
2) The DNA chain acting as a template for in vitro RNA synthesis has the sequence 5' - AGTCTTAATCCTG-3'. What is the sequence of the synthesized mRNA?

[Question ID = 52169]
--- Content provided by‌ FirstRanker.com ---
1. 5' CAGGATTAAGACT [Option ID = 88668]
2. 5' CAGGAUUAAGACU [Option ID = 88671]
3. 5' UCAGAAUUAGGAC [Option ID = 88670]
4. 5' TCAGAATTAGGAC [Option ID = 88669]
Correct Answer :-
--- Content provided by⁠ FirstRanker.com ---

5' CAGGAUUAAGACU [Option ID = 88671]
3) The relationship between genomes of helper virus and satellite virus is best explained as:

[Question ID = 52198]
1. Satellite virus originated from helper virus [Option ID = 88784]
2. Satellite viruses have no homology to helper virus [Option ID = 88786]
3. Helper virus originated from satellite virus [Option ID = 88785]
4. None of these [Option ID = 88787]
Correct Answer :-

Satellite viruses have no homology to helper virus [Option ID = 88786]
--- Content provided by‌ FirstRanker.com ---
4) The bright orange color of cheddar cheese is due to the presence of following seed extract:

[Question ID = 52166]
1. Humulus lupulus seed [Option ID = 88657]
2. Annatto and Humulus lupulus seed [Option ID = 88659]
3. Annatto seed [Option ID = 88656]
4. Dill seeds [Option ID = 88658]
Correct Answer :-

Annatto seed [Option ID = 88656]

--- Content provided by FirstRanker.com ---

5) The rate of sedimentation of spherical particles suspended in a fluid of Newtonian viscosity:

[Question ID = 52191]
1. Inversely proportional to the square of the particle density [Option ID = 88759]
2. Inversely proportional to the square of the diameter of the particles [Option ID = 88757]
3. Inversely proportional to the square of the gravitational constant [Option ID = 88758]
4. Directly proportional to the square of the diameter of the particles [Option ID = 88756]
Correct Answer:

Directly proportional to the square of the diameter of the particles [Option ID = 88756]
6) The rate of flow during gravity based batch filtration is:
--- Content provided by⁠ FirstRanker.com ---

[Question ID = 52205]
1. Inversely proportional to the depth of filter bed [Option ID = 88813]
2. Inversely proportional to the area of filter bed exposed to liquid [Option ID = 88815]
3. Directly proportional to the depth of filter bed [Option ID = 88812]
4. Directly proportional to the area of filter bed exposed to liquid [Option ID = 88814]
Correct Answer :-

Inversely proportional to the depth of filter bed [Option ID = 88813]
7) The mRNA will form hybrids only with the coding strand of DNA because:

[Question ID = 52206]
--- Content provided by‍ FirstRanker.com ---
1. The salt concentration will affect DNA re-annealing [Option ID = 88817]
2. DNA will not re-anneal at low temperatures [Option ID = 88818]
3. DNA will not re-anneal at high temperatures [Option ID = 88816]
4. RNA:DNA hybridization follows the base-pairing rules [Option ID = 88819]
Correct Answer :-
--- Content provided by FirstRanker.com ---

RNA:DNA hybridization follows the base-pairing rules [Option ID = 88819]
8) The optimum biotin concentration in the growth medium of Corynebacterium glutamicum will help in excretion of:

[Question ID = 52202]
1. Acetate [Option ID = 88803]
2. Aspartate [Option ID = 88802]
3. Lactate [Option ID = 88801]
4. Glutamate [Option ID = 88800]
Correct Answer :-

Lactate [Option ID = 88801]
--- Content provided by⁠ FirstRanker.com ---
9) The causative agent of syphilis, a sexually transmitted disease (STD):

[Question ID = 52186]
1. Neisseria sp. [Option ID = 88737]
2. Borrelia burgdorferi [Option ID = 88739]
3. Treponema pallidum [Option ID = 88738]
4. Gonorrhea sp. [Option ID = 88736]
Correct Answer :-

Treponema pallidum [Option ID = 88738]

--- Content provided by⁠ FirstRanker.com ---

10) Who were awarded the Nobel prize in 1962 for discovering the structure of DNA?

[Question ID = 53375]
1. Watson and Crick [Option ID = 93486]
2. Watson, Crick and Wilkins [Option ID = 93489]
3. Watson, Crick and Pauling [Option ID = 93488]
4. Watson, Crick and Franklin [Option ID = 93487]
Correct Answer :-

Watson, Crick and Wilkins [Option ID = 93489]
11) In positive control in an inducible operon, indicate whether the protein produced by the regulator gene will be synthesized initially as:
--- Content provided by⁠ FirstRanker.com ---

[Question ID = 52162]
1. an active repressor [Option ID = 88640]
2. an active activator [Option ID = 88642]
3. an inactive activator. [Option ID = 88643]
4. an inactive repressor [Option ID = 88641]
Correct Answer :-

an inactive activator. [Option ID = 88643]
12) In positive control in a repressible operon, indicate whether the protein produced by the regulator gene will be synthesized initially as:

[Question ID = 52160]
--- Content provided by FirstRanker.com ---
1. an active repressor [Option ID = 88632]
2. an active activator [Option ID = 88634]
3. an inactive activator. [Option ID = 88635]
4. an inactive repressor [Option ID = 88633]
Correct Answer :-
--- Content provided by⁠ FirstRanker.com ---

an active activator [Option ID = 88634]
13) In negative control in an inducible operon, indicate whether the protein produced by the regulator gene will be synthesized initially as:

[Question ID = 52163]
1. an active repressor [Option ID = 88644]
2. an active activator [Option ID = 88646]
3. an inactive activator. [Option ID = 88647]
4. an inactive repressor [Option ID = 88645]
Correct Answer :-

an active repressor [Option ID = 88644]
--- Content provided by FirstRanker.com ---
14) In negative control in a repressible operon, indicate whether the protein produced by the regulator gene will be synthesized initially as:

[Question ID = 52161]
1. an active repressor [Option ID = 88636]
2. an active activator [Option ID = 88638]
3. an inactive activator. [Option ID = 88639]
4. an inactive repressor [Option ID = 88637]
Correct Answer :-

an inactive repressor [Option ID = 88637]

--- Content provided by FirstRanker.com ---

15) Positive sense RNA virus genomes are not coated with proteins except in case of:

[Question ID = 52196]
1. Retroviruses [Option ID = 88776]
2. Flaviviruses [Option ID = 88778]
3. Picornaviruses [Option ID = 88779]
4. Rotaviruses [Option ID = 88777]
Correct Answer :-

Retroviruses [Option ID = 88776]
16) Antibodies directed against which of the following components of LPS (present in the cell wall of gram-negative bacteria) determine the serotype of the bacteria?
--- Content provided by FirstRanker.com ---

[Question ID = 52170]
1. O-specific side chain [Option ID = 88674]
2. Lipid A [Option ID = 88673]
3. Core oligosaccharide [Option ID = 88672]
4. Techoic acid [Option ID = 88675]
Correct Answer :-

O-specific side chain [Option ID = 88674]
17) Antibiotic erythromycin disrupts protein synthesis by preventing translocation. Assuming you are performing in vitro translation and you add erythromycin after the formation of the 10th peptide bond but before the formation of the 11th peptide bond, which of the following statements would be correct?

[Question ID = 52176]
--- Content provided by⁠ FirstRanker.com ---
1. tRNA in the P site would not be charged with any amino acid and eleven amino acids would be attached to the tRNA in the A site. [Option ID = 88697]
2. Eleven amino acids would be attached to the tRNA in the P site while the A site would be empty. [Option ID = 88699]
3. Ten amino acids would be attached to the tRNA in the P site and only one amino acid would be attached to tRNA in the A site. [Option ID = 88696]
4. Neither A site nor P site would carry any tRNA. [Option ID = 88698]
Correct Answer :-
--- Content provided by‌ FirstRanker.com ---

Ten amino acids would be attached to the tRNA in the P site and only one amino acid would be attached to tRNA in the A site. [Option ID = 88696]
18) Lactose transport through E. coli membrane is an example of:

[Question ID = 52183]
1. Secondary active transport [Option ID = 88725]
2. Primary active transport [Option ID = 88724]
3. Facilitative diffusion [Option ID = 88726]
4. Group translocation [Option ID = 88727]
Correct Answer :-

Secondary active transport [Option ID = 88725]
--- Content provided by FirstRanker.com ---
19) Supramolecular "injectisome" is produced during:

[Question ID = 52182]
1. Phage mediated injection of DNA [Option ID = 88722]
2. All of these [Option ID = 88723]
3. Type III secretion system (TTSS / T3SS) of bacteria [Option ID = 88721]
4. Type V secretion system of bacteria [Option ID = 88720]
Correct Answer :-

Type III secretion system (TTSS / T3SS) of bacteria [Option ID = 88721]

--- Content provided by‌ FirstRanker.com ---

20) Clostridium difficile commonly known as C. diffis known to be associated with:

[Question ID = 52195]
1. MRSA [Option ID = 88774]
2. Antibiotic related diarrhea [Option ID = 88773]
3. Surgical site infections [Option ID = 88772]
4. TORCH group of pathogens [Option ID = 88775]
Correct Answer :-

Antibiotic related diarrhea [Option ID = 88773]
21) Eukaryotic myoglobin protein is composed of 150 amino acids. What would be the number of nucleotides in the open reading frame in the corresponding mRNA. [Question ID = 52167]
--- Content provided by‌ FirstRanker.com ---
1. 450 bases [Option ID = 88661]
2. 453 bases [Option ID = 88660]
3. 900 bases [Option ID = 88663]
4. 447 bases [Option ID = 88662]
Correct Answer :-
--- Content provided by⁠ FirstRanker.com ---

453 bases [Option ID = 88660]
22) Four trp operon mutants were isolated. Mutant A carries a trp repressor which cannot bind the operator sequence even in the presence of tryptophan. Mutant B expresses a trp repressor protein that is unable to bind tryptophan. Mutant C carries a mutation in the ribosome binding site at the 5' end of the mRNA and a trp repressor that cannot bind to the operator even in the presence of tryptophan. Which mutant cannot be "rescued" by expressing wild type trp repressor in the cells?
1. Mutant B [Option ID = 88717]
2. Mutant C [Option ID = 88718]
3. Mutant A [Option ID = 88716]
4. None of these [Option ID = 88719]
Correct Answer :-

Mutant C [Option ID = 88718]

--- Content provided by FirstRanker.com ---

23) Single molecule real time sequencing uses f29 DNA polymerase because:

[Question ID = 52187]
1. It has high fidelity. [Option ID = 88741]
2. It has exceptionally high processivity. [Option ID = 88740]
3. All of these [Option ID = 88743]
4. It can incorporate phospho-linked nucleotides. [Option ID = 88742]
Correct Answer :-

All of these [Option ID = 88743]
24) The rate of enzyme catalysed reaction was measured using several substrate concentrations that were much lower than Km. The dependence of enzyme velocity can be best explained as:
--- Content provided by‍ FirstRanker.com ---

[Question ID = 52197]
1. Proportional to substrate concentration [Option ID = 88781]
2. A constant fraction of Vmax [Option ID = 88782]
3. Zero order with respect to substrate concentration [Option ID = 88783]
4. Independent of enzyme concentration [Option ID = 88780]
Correct Answer :-

Proportional to substrate concentration [Option ID = 88781]
25) The following is the sum of three steps in the citric acid cycle:

A + B + FAD + H2O ? C + FADH2 + NADH
--- Content provided by‌ FirstRanker.com ---

A, B, C stands for:

[Question ID = 52190]
1. a ketoglutarate, NAD+, succinate [Option ID = 88754]
2. Succinate, NAD+, malate [Option ID = 88755]
3. Succinate, NAD+, oxaloacetate [Option ID = 88753]
4. Succinyl CoA, GDP, succinate [Option ID = 88752]
Correct Answer :-

Succinate, NAD+, oxaloacetate [Option ID = 88753]
26)
--- Content provided by⁠ FirstRanker.com ---

If A and B microorganisms are present in a continuous enrichment culture, limited by the substrate, then which organism will be selected above a specific growth rate of Y

[Question ID = 52209]
1. Both [Option ID = 88830]
2. Neither A nor B [Option ID = 88831]
3. Organism B [Option ID = 88829]
4. Organism A [Option ID = 88828]
Correct Answer :-

Organism B [Option ID = 88829]
27) A protein sample of 2mg/ml concentration was provided to a class. Students were asked to calculate molar concentration of the sample if molecular weight of the protein is 50 kDa. The class came up with 4 different values. Choose the correct option
--- Content provided by‍ FirstRanker.com ---

Value 1 Value 2 Value 3 Value 4

40 µM 4 x 10^-3 M 0.04 mM 4 x 10^-5 M

[Question ID = 52199]
1. Values 1,3 and 4 [Option ID = 88790]
2. Only value 1 [Option ID = 88789]
3. Only value 4 [Option ID = 88791]
4. Both values 2 and 3 [Option ID = 88788]
Correct Answer :-

Values 1,3 and 4 [Option ID = 88790]

Value 1	Value 2	Value 3	Value 4
40 µM	4 x 10^-3 M	0.04 mM	4 x 10^-5 M

28) You perform a BLAST search using the sequence of a new virus you have isolated. You get the following results. What can you determine from these results?

Score E Sequences producing significant alignments	Total Score
gi\|28416271\|gb\|AC121946.3 \| Yellow fever capsid protein	25
gi\|68072784\|ref\|XM_673214.1\| Dengue fever virus 1 capsid protein	400
gi\|70951865\|ref\|XM_740047.1\| Dengue fever virus 2 capsid protein	768
gi\|23495940\|gb\|AE014836.1 \| Dengue fever virus 3 capsid protein	954

[Question ID = 52193]

--- Content provided by⁠ FirstRanker.com ---

These results tell you nothing about how related your virus is to these viruses [Option ID = 88767]
Your new virus is equally related to dengue fever virus 1, 2, and 3 [Option ID = 88764]
Your new virus is most closely related to yellow fever virus [Option ID = 88766]
Your new virus is most closely related to dengue fever virus 3 [Option ID = 88765]

Correct Answer :-

--- Content provided by FirstRanker.com ---

Your new virus is most closely related to dengue fever virus 3 [Option ID = 88765]

29) Match the following:

Column A	Column B
I. ChIP	(i) Mutagenesis
II. BIFC	(ii) Protein-DNA interaction
III. Alpha-complementation	(iii) Global gene expression
IV. SAGE	(iv) Protein-protein interaction
	(v) Selection of recombinants

[Question ID = 52188]

I-(ii), II-(iii), III-(v), IV-(iv) [Option ID = 88744]

--- Content provided by FirstRanker.com ---

I-(ii), II-(iii), III-(v), IV-(iv) [Option ID = 88747]
I-(ii), II-(iv), III-(v), IV-(iii) [Option ID = 88746]
I-(iv), II-(ii), III-(i), IV-(iii) [Option ID = 88745]

Correct Answer :-

I-(ii), II-(iv), III-(v), IV-(iii) [Option ID = 88746]

--- Content provided by⁠ FirstRanker.com ---

30) A student was asked to study the effect of Ca²⁺ ions on enzyme kinetics and thermal stability at 50°C (molecular weight of the enzyme is 30 kDa). The data was tabulated and plotted as given below and the results were interpreted as follows

Sample V_max (µmol/mg/min) K_m (mol/L) K_d

In absence of Ca 40 0.50 -0.03

In presence of Ca 40 0.35 -0.01
1. K_cat is unaltered but catalytic efficiency is lowered in the presence of Ca²⁺
2. Ca²⁺ stabilizes the enzymes at 50°C and also increases the affinity of enzyme towards substrate
3. The enzyme has higher t_1/2 in presence of Ca²⁺ at 50°C and its K_cat value is 1.2 x 10³ min^-1
4. The enzyme has higher t_1/2 in presence of Ca²⁺ at 50°C and its K_cat value is 1.33 min^-1
Which of the following combination of statement(s) is correct?

[Question ID = 52204]
1. 2 and 4 [Option ID = 88810]
2. 2 and 3 [Option ID = 88809]
3. 1 and 4 [Option ID = 88811]
4. 1, 2 and 3 [Option ID = 88808]
Correct Answer :-

2 and 3 [Option ID = 88809]

Sample	V_max (µmol/mg/min)	K_m (mol/L)	K_d
In absence of Ca	40	0.50	-0.03
In presence of Ca	40	0.35	-0.01

--- Content provided by‌ FirstRanker.com ---

31) In a CSTR process, the dilution rate of the process was set at 0.25 h^-1, whereas the flow rate of fresh medium was set at 800 ml/h. Calculate the working volume of the process at a specific biomass yield of Y_p/x of 0.5.
32) V-D-J rearrangement is responsible for generating diversity of:

[Question ID = 52201]
1. T cell receptors (TCR) only [Option ID = 88798]
2. Immunoglobulin variable region only [Option ID = 88796]
3. Immunoglobulin variable and constant region only [Option ID = 88797]
4. Both Immunoglobulin variable region and TCR [Option ID = 88799]
Correct Answer :-

Both Immunoglobulin variable region and TCR [Option ID = 88799]
--- Content provided by FirstRanker.com ---
33) When virulent viruses are repeatedly cultured for many passages in cell culture system, then:

[Question ID = 52185]
1. Viruses evolve to grow efficiently in cell culture and may lose sequences associated with pathogenicity and get attenuated [Option ID = 88734]
2. Virulent viruses cannot be passaged in cell culture [Option ID = 88735]
3. Viruses become more virulent and pathogenic [Option ID = 88733]
4. Viruses lose their ability to grow in cell culture after few passages [Option ID = 88732]
Correct Answer :-

Viruses evolve to grow efficiently in cell culture and may lose sequences associated with pathogenicity and get attenuated [Option ID = 88734]

--- Content provided by FirstRanker.com ---

34) You have isolated an E. coli mutant which lyses faster than the wild type strain in hypotonic solution. A defect in which of the following proteins in the mutant strain would manifest such a result?

[Question ID = 52179]
1. Porin protein [Option ID = 88708]
2. Aquaporin [Option ID = 88709]
3. ABC transporter [Option ID = 88711]
4. Mechanosensitive channel [Option ID = 88710]
Correct Answer :-

Mechanosensitive channel [Option ID = 88710]
35) Major histocompatibility class I (MHC-I) molecules are present on:
--- Content provided by⁠ FirstRanker.com ---

[Question ID = 52189]
1. All nucleated cells [Option ID = 88750]
2. Antigen presenting cells (APCs) only [Option ID = 88749]
3. B lymphocytes but not on T lymphocytes [Option ID = 88751]
4. B lymphocytes only [Option ID = 88748]
Correct Answer :-

All nucleated cells [Option ID = 88750]
36) Towards deciphering the genetic code Dr. Har Gobind Khorana developed a method to synthesize long RNA molecules consisting of short defined sequence repeated many times. Assuming 5' UUAC sequence is repeated over and over, how many different triplets would occur in synthetic RNA?

[Question ID = 52178]
--- Content provided by⁠ FirstRanker.com ---
1. UUA, CUU, ACU, UAC [Option ID = 88707]
2. UUA. CAU, UAC, ACU [Option ID = 88706]
3. CAU, AUU, UUC, UCA [Option ID = 88704]
4. CUU, ACU, CAU, UUU [Option ID = 88705]
Correct Answer :-
--- Content provided by‍ FirstRanker.com ---

UUA, CUU, ACU, UAC [Option ID = 88707]
37) A sample of 0.2 ml of purified protein is diluted to a total volume of 1.0 ml in a cuvette having a 1 cm light path. The absorbance of the sample at 280 nm was 0.50. What is the protein concentration in the original sample? Consider 1 absorbance unit at 280 nm equal to 1 mg protein/ml.

[Question ID = 52165]
1. 1.50 mg/ml [Option ID = 88655]
2. 1.0 mg/ml [Option ID = 88652]
3. 2.50 mg/ml [Option ID = 88654]
4. 1
  This download link is referred from the post: DUET Last 10 Years 2011-2021 Question Papers With Answer Key || Delhi University Entrance Test conducted by the NTA

This post was last modified on 29 January 2020

Get the MBBS Question Bank Android App

Download DUET Master 2018 DU PhD in Microbiology Question Paper With Answer Key

Share This Post:

Share This Post: