Max. Marks: 80 Marks
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INSTRUMENTAL & BIO-MEDICAL ANALYSIS
(Revised Scheme - 2)
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Q.P. CODE: 1967Your answers should be specific to the questions asked
Draw neat labeled diagrams wherever necessary
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LONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
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1.Compare the principle, technique, limitations and applications of paper chromatography
with paper electrophoresis.
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2.Describe the construction and working, advantages, disadvantages and applications of a
standard hydrogen electrode.
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3.Enumerate detectors used in IR spectrometers. Explain with the diagrams, the principle
involved in the working of thermal detectors in IR spectroscopy.
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SHORT ESSAYS (Answer any Eight)8 x 5 = 40 Marks
4.
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Describe the different analytical methods of determining end-point in potentiometric
titrations.
5.
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What is quenching? Explain various types of quenching with suitable examples.
6.
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What is the principle underlying conductometric titration? Explain titration curves fordisplacement conductometric titration with examples.
7.
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Describe and classify ion exchange resins. Explain the factors that determine the
distribution of ions between an ion exchange resin and a solution.
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8.Explain two - dimensional and reverse phase chromatography. Emphasize the application of
these techniques.
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9.
What are monochromators? Describe the principle underlying the production of
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monochromatic radiations using prisms and gratings.10.
What is fluorescence and phosphorescence? Explain the concept of fluorescence through
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energy level diagram.
11.
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Derive an equation for "Half wave potential".12.
What is group frequency region and finger print region in an infrared spectrum? Write
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characteristic fundamental infrared absorption wave numbers for following compounds:a. Aniline b. Acetone
13.
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With the help of titration curves, describe the principles of potentiometric titrations.
SHORT ANSWERS
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10 x 2 = 20 Marks14.
Draw the spectrophotometric titration curve for a system wherein the product alone
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absorbs, while the substance titrated and titrant are not absorbing the radiation.
15.
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Explain the significance of the two filters in a fluorimeter.16.
Write the advantages of thin-layer chromatography over paper chromatography.
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17.
What is programmed temperature gas chromatography?
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18.State Lambert ? Beer's law and write its mathematical expression.
19.
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What is chemical shift?
20.
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What is the role of a supporting electrolyte in polarography?21.
What are the advantages of a double beam spectrophotometer over a single beam
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instrument?
22.
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Define indicator and reference electrode. Give examples.23.
Explain absorption maxima. How it is determined?
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