Max. Marks: 70 Marks
--- Content provided by FirstRanker.com ---
INSTRUMENTAL & BIO-MEDICAL ANALYSIS
(Revised Scheme ? 3)
--- Content provided by FirstRanker.com ---
Q.P. CODE: 2617Your answers should be specific to the questions asked
Draw neat labeled diagrams wherever necessary
--- Content provided by FirstRanker.com ---
LONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
--- Content provided by FirstRanker.com ---
1.Discuss the constitution of different ion exchange materials used in chromatography and
explain the principle involved in the separation of mixtures by ion exchange chromatography.
--- Content provided by FirstRanker.com ---
2.
Draw a neat, labeled schematic diagram of a double beam UV ? Visible spectrophotometer and
--- Content provided by FirstRanker.com ---
explain its working. Discuss the construction and working of photo multiplier tube and silicondiodes used in UV-Visible spectrophotometers.
3.
--- Content provided by FirstRanker.com ---
a) With the help of a neat, labeled diagram, explain the optical path and working of aspectrofluorimeter.
b) What are the structural features required for a compound to show fluorescence?
--- Content provided by FirstRanker.com ---
SHORT ESSAYS (Answer any Six)6 x 5 = 30 Marks
4.
--- Content provided by FirstRanker.com ---
Discuss the merits and demerits of potentiometric titrations over conductometric titrations.
5.
--- Content provided by FirstRanker.com ---
What shape of curve do you expect for each of the conductometric titrations? Justify youranswer.
(a)Sodium acetate versus HCl; (b)Sulphuric acid versus Ammonia followed by NaOH.
--- Content provided by FirstRanker.com ---
6.Give a mathematical expression to show the relation between absorbance and concentration.
Explain the different modes of expressing concentration.
--- Content provided by FirstRanker.com ---
7.Briefly explain validation methods for quality assurance.
8.
--- Content provided by FirstRanker.com ---
Explain the principle of flame emission spectroscopy. What are the various components present
in a flame emission spectrophotometer?
--- Content provided by FirstRanker.com ---
9.Give the wavelength ranges for vacuum UV, UV, visible, near IR, mid IR and far IR radiations
and mention their relative energies.
--- Content provided by FirstRanker.com ---
10.
What is max? Explain why, it is selected in quantitative UV ? Visible spectrophotometry.
--- Content provided by FirstRanker.com ---
11.Highlight the differentiating features of preparative TLC from analytical TLC.
SHORT ANSWERS
--- Content provided by FirstRanker.com ---
10 x 2 = 20 Marks
12.
--- Content provided by FirstRanker.com ---
Calculate the Rm value of glucose, if its Rf value is 0.25.13.
Give any two official pharmaceutical applications of gas chromatography.
--- Content provided by FirstRanker.com ---
14.
Name any four detectors used in IR spectrophotometers.
--- Content provided by FirstRanker.com ---
15.What is isosbestic point? What is its significance in UV-Visible spectrophotometry?
16.
--- Content provided by FirstRanker.com ---
Why, nephelometric measurements of scattered light are, carried - out, at right angles to the
incident light?
--- Content provided by FirstRanker.com ---
17.Explain the effect of pH on fluorescence.
18.
--- Content provided by FirstRanker.com ---
Which reference and indicator electrodes can be used for the following potentiometric
titrations:
(a) Acid-Base titrations, (b) Red-ox titrations?
--- Content provided by FirstRanker.com ---
19.
Give the path lengths generally used for liquids and gases in IR spectroscopy.
--- Content provided by FirstRanker.com ---
20.Which functional groups are indicated for IR absorption peaks of 1700 cm-1 and 3450 cm-1?
21.
--- Content provided by FirstRanker.com ---
Outline the principle of electrophoresis.
*****
--- Content provided by FirstRanker.com ---