Max. Marks: 70 Marks
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Instrumental and Bio-Medical Analysis
(RS ? 4)
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Q.P. CODE: 2642Your answers should be specific to the questions asked
Draw neat labeled diagrams wherever necessary
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LONG ESSAYS (Answer any Two)
2 x 10 = 20 Marks
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1.Describe the construction and working of a double-beam spectrophotometer with a neat
diagram and its applications.
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2.
Discuss in detail about rate theory with support of Vandeemter equation. Narrate about the
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pumps used in HPLC.3.
Discuss the determination of rate constant by spectroscopy. Explain briefly about sample cells
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and radiation sources in IR.
SHORT ESSAYS (Answer any Six)
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6 x 5 = 30 Marks4.
Explain the reasons for Beer's law?
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5.
Describe the factors which affect the fluorescence intensity.
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6.Briefly explain the different detection methods of paper chromatography
7.
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Write the theoretical aspects of electophoresis for separation of molecular mixture
8.
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Write the construction and working of Glass electrode with advantages and disadvantages9.
Explain the conductometric titration curves for weak acid with strong base, weak acid with
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weak base and very weak acid with strong base.
10.
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What is flame emission photometry? Write the theoretical aspects of flame emissionphotometry.
11.
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What are nephelometry and Turbidametry? Write principle involved for the same.
SHORT ANSWERS
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10 x 2 = 20 Marks12.
What is multicomponent analysis? Write the application of beer's law in analysis of drugs.
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13.
Name the fuel gases used in flame emission spectroscopy.
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14.Write the block diagram of Nephelometer
15.
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Write the difference between normal phase and reverse phase chromatography
16.
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Write the composition and types of paper used in paper chromatography.17.
Write the difference between isocratic and gradient elution
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18.
Name the detection methods in HPTLC
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19.What are the applications of NMR spectroscopy?
20.
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Write the important application of x-ray diffraction spectroscopy
21.
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Write the difference between quality control and quality assurance.*****