Rajiv Gandhi University of Health Sciences
First Semester M. Pharm Degree Examination - FirstRanker.com
[Time: 3 Hours] [Max. Marks: 75]
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Cellular and Molecular Pharmacology
Q.P. CODE: 5136
Your answers should be specific to the questions asked.
Draw neat, labeled diagrams wherever necessary.
LONG ESSAY (Answer any Three) 3 X 10 = 30 Marks
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- Describe the signalling pathway via GPCR and add a note on its regulation.
- Explain cell viability test using MTT assay and describe the working principles and applications of flow cytometry.
- Discuss the structure of gene and explain briefly the steps involved in gene expression. Explain any one method for genomic mapping.
- Explain the basic principles and applications of Western blotting and PCR.
SHORT ESSAY (Answer any Nine) 9 X 5 = 45 Marks
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- Explain any one method for determination of calcium influx assay.
- What are cloning vectors and give their role in rDNA technology?
- What is microarray technique? Mention the various applications.
- Explain the Sanger’s method of gene sequencing.
- Discuss the principles of glucose uptake assay.
- What is cryopreservation and describe technique of cryopreservation of cell lines.
- Explain cell signalling through tyrosine kinase receptors.
- Explain the role of IP3 and DAG in pharmacology.
- What is genomics and proteomics? What are their limitations?
- Write the structure and functions of plasma membrane.
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