MCQ Section A will be given to the candidate at the beginning of the examination. After 30
minutes Section A will be collected . Paper containing Section B and Section C will then be
handed over to the candidate. Section B and Section C is to be written in separate answer
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sheets.SECTION -A
Q.No. 1. Multiple choice questions (MCQs 30)
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(30 minutes duration) 15 marks.SECTION -B
Q.No. 2.Write in brief (Any five out of six) 2marks each 10 marks.
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Q.No 3. On applied Biochemistry (Any two out of three ) 8 marks.SECTION-C
Q.No.4. Long question (A) 9 marks.
OR
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Long question (B )Q. No.5 . Write short notes(any two out of three) 8 marks.
6. PRACTICAL :
Practical examination in Biochemistry will be of
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TWO hours duration 40 marksB) Exercise
Q.1. : One quantitative experiment from group A 20 marks
(15 marks for expt. & 5 marks for table viva)
Q.2. : One qualitative/ quantitative experiment from 15 marks
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group B.(10 marks for expt. & 5 marks for table vivaQ.3. Spot identification from group C. 5 marks.
Group A :
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Blood sugar, Blood urea; Serum total protein, Albumin and A/G ratio, Alanineamino transaminase(SGPT), Aspartate amino transaminase(SGOT) , Alkaline phosphatase,
Serum amylase, Serum total bilirubin, Serum uric acid, Serum calcium, CSF sugar.
Group B :
Creatinine in urine, Serum cholesterol, Serum phosphorus, CSF protein, Tests for
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monosaccharides ( Ben edict, Barfoed,Selivanoff, Nylander, rapid furfural) , Testsfor disaccharides, Colour reactions of proteins, Precipitation reactions of proteins,
Normal Organic constituents of urine, Abnormal constituents of urine.
Group C :
Identification of slide under microscope, Use of reagent. Significance of test.
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Use of Instrument /Appliances. Identification of Hb - derivative. Identification ofGTT , Electrophoretogram and chromatogram.
Candidate will be allowed to use flow chart for quantitative exercise only.
There will be table viva on Q.1 & Q.2 exercise.
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7. SYLLABUS FOR PRACTICAL1. Tests for monosaccharides.
2. Tests for disaccharides.
3. Colour reactions of proteins.
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4. Precipitation reactions of proteins.5. Spectroscopic examination of Hb -derivatives (Oxy Hb; deoxy Hb; meth-Hb ).
6. Estimation of blood sugar.
7. Estimation of blood urea.
8. Estimation of i) Serum creatinine, ii) Creatinine in urine..
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9. Determination of serum total protein ,albumin and A/G ratio.10. Estimation of total serum bilirubin.
11. Estimation of serum cholesterol.
12. Estimation of serum calcium.
13. Estimation of serum phosphorus ( Inorganic)
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14. Estimation of S.G.P.T( ALT ).15. Estimation of S.G.O.T (AST ).
16. Estimation of serum alkaline phosphatase.
17. Estimation of serum amylase.
18. Urine ; Physical characteristics and normal constituents ( organic )
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19. Urine report; Physical characteristics and Abnormal constituents.20. C.S.F.- Sugar & Protein.
21. Serum uric acid.
Lecture ?cum- Demonstrations :
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1. PH- measurement,2. Colorimetry.
3. Electrophoresis.
4. Chromatography.
5. Flame photometry.
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