Rajiv Gandhi University of Health Sciences, Karnataka
ITI Year B.Sc. (M.L.T) Degree Examination - MAY 2016
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Time: Three Hours Max. Marks: 80 Marks
BIOCHEMISTRY - III (RS-2)
Q.P. CODE: 2901
Your answers should be specific to the questions asked.
Draw neat, labeled diagrams wherever necessary
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LONG ESSAYS (Answer any Two) 2 x 10 = 20 Marks
- Define isoenzyme. Define any two units of enzyme activity. Explain the diagnostic importance of enzymes in pancreatic and bone disease.
- Classify plasma proteins. Write the procedure and neatly labelled diagram of electrophoresis separation of serum proteins.
- Describe the sources, daily requirement and normal levels of calcium. Enumerate any five functions of calcium. Explain briefly how calcium level is regulated.
SHORT ESSAYS (Answer any Six) 6 x 5 = 30 Marks
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- Enumerate the functions of liver.
- Write the procedure and utility of ELISA.
- Explain the regulation of acid base balance.
- Enumerate any three cardiac biomarkers. Explain the chronobiogram and clinical utility of cardiac troponin.
- Classify the liver function test with examples.
- Explain briefly how to manually analyse renal stones.
- Write the principle and applications of radio immuno assay.
- Write the normal level of sodium. Write any two causes of hyponatremia and hypernatremia.
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SHORT ANSWERS (Answer any Ten) 10 x 3 = 30 Marks
- Enumerate the inhibitors of transcription and translation.
- Enumerate any one cause each of Hyperkalemia and Hypokalemia.
- What is coefficient of variation? Explain with suitable example.
- Enumerate different forms and location of creatinine kinase.
- Enumerate the diagnostically important enzyme in liver disease.
- Write LJ chart with a suitable example.
- Enumerate the enzymes used to diagnose pancreatitis and write their normal values.
- Define and write the normal levels of anion gap.
- What is the utility of PCR?
- What are the causes of congenital jaundice?
- Enumerate the steps of DNA replication.
- Enumerate the steps of protein synthesis.
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