Rajiv Gandhi University of Health Sciences
M.D. Degree Examination - MAY-2019
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[Time: 3 Hours] [Max. Marks: 100]
MICROBIOLOGY
PAPER -1
Q.P. CODE: 7355
Your answers should be specific to the questions asked.
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Draw neat, labeled diagrams wherever necessary.
Answer all the Questions 10 X 10 = 100 Marks
- Classify and describe the principles of the Polymerase Chain Reaction and critically evaluate their value in diagnostic microbiology. Add a note on RT-PCR.
- Outline the general properties of cytokines. Enumerate the three cytokines which play a major role in the acute phase response and describe how they act to combat infection.
- Role played by a microbiologist in occupational exposure of blood and body fluids in a health care setting.
- Describe the criteria for assessing and the control measures you would adopt to ensure the quality of culture media in a diagnostic laboratory.
- List the mechanisms through which a change may occur in the genome of a bacterial cell. Describe any one of these mechanisms. Explain how these mechanisms lead to the spread of antimicrobial resistance.
- What is an antibiotic policy? Discuss stating advantages and disadvantages and how you would develop an antibiotic policy for use in a medical college.
- Cleaning and sterilization procedure for glassware used in microbiology.
- Discuss the role of B cells in immune response.
- Use of monoclonal antibodies in diagnostic microbiology.
- Spaulding classification for sterilization.
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