RUHS
First Year MBBS Examination
I MBBS BIOCHEMISTRY PAPER II
Max
Time: 3 hours

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Marks:
100
Date: 22-11-2023
Instructions: INSTRUCTIONS: Attempt
all questions in both sections: (Use

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separate answer book for each section)
Section 1
1. Fill in the blanks: (6)
a. The enzyme __________ is a ribozyme which
catalyzes peptide bond formation.

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b. The mutated genes capable of causing
cancer are called __________.
c. __________ is an antibody-based laboratory
technique used to detect a specific protein in
a blood or tissue sample.

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d. Hereditary nonpolyposis colorectal cancer
occurs due to defective __________ repair.
e. The enzyme __________ catalyzes the
glucuronidation of bilirubin.
f. Bacterial artificial chromosomes can carry

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genomic DNA insert up to __________.

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2. Choose the correct option in the following
multiple choice questions: (4)
a. Sickle-cell is an example of:
a. Silent mutation

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b. Missense mutation
c. Nonsense mutation
d. None of the above
b. Which of the following is accessed by the
Creatinine clearance test?

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a. Renal concentration capacity
b. Renal dilution capacity
c. Glomerular function
d. Tubular function
c. which of the following antibody is found in

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tears?
a. IgG
b. IgA
c. IgM
d. IgE

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d. The essential amino acid limiting in rice is:
a. Methionine
b. Tryptophan
c. Lysine
d. Histidine

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3. Clinical case study: A 26-year-old female is
two months post-partum with her first

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pregnancy. Infant was carried to term without
complications. She complained of (15) extreme
fatigue, weight gain, constipation, cold

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intolerance and feelings of inadequacy as a
mother. The clinician noted that the deep
tendon reflex time was delayed. Blood sample
was collected. Laboratory result were as
follows: Glucose: 92.0 mg/dL, Cholesterol:

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192.0 mg/dL, Triglycerides: 190.0 mg/dL, TSH:
10.0 plU/mL, FT3: 1.8 pg/mL, FT4: 0.54 ng/dL,
TPOAb: 5.5 IU/mL (ref range < 9.0 IU/mL),
TgAb: 2.5 IU/mL (ref range < 4.0 (U/mL).
a. What is the most probable diagnosis?

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b. What is the rationale behind the above
diagnosis?
c. Which additional signs and symptoms should
be taken into consideration?
d. What are the most likely causes of this

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disease?
e. Write down the normal biological reference
range of Free T3, Free T4 and TSH is th
serum of healthy adults?
4. Write short notes on (Any five): (10)

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a. DNA polymerases in eukaryotes and their
functions
b. Antioxidant enzymes

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c. Restriction endonucleases
d. Difference between glycemic index and

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glycemic load
e. Applications of Southern blotting technique
f. Okazaki segments
5. Explain briefly (Any three): (15)
a. Gene library

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b. Immune response
c. Mutation
d. Lac Operon

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Section 2
6. Explain the process of transcription in detail.

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Describe the post transcriptional modifications.
Write a note on the inhibitors of transcription.
(20)
7. Explain why (Any five): (10)
a. Puromycin inhibits protein synthesis.

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b. A combination of pulses and cereals become
equivalent to first class protein.
c. Serum Malondialdehyde acts as a marker of
oxidative stress.
d. p53 functions as a tumour suppressor gene.

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e. Malignant cells develop drug resistance to
long term administration of methotrexate.
f. Urobilinogen in absent from urine in
obstructive jaundice.
8. Explain briefly (Any four): (0)

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a. Balanced diet
b. Hybridoma technology
c. Tumor markers
d. ELISA
e. Restriction fragment length polymorphism

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