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[LC 814] APRIL 2013 Sub. Code: 3814
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DOCTOR OF PHARMACY (PHARM. D) DEGREE EXAMINATION
THIRD YEAR
PAPER II - PHARMACEUTICAL ANALYSIS
Q.P. Code: 383814
Time: Three Hours Maximum: 100 marks
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Answer All questions
- Elaborate on: (2x20=40)
- Explain the working and components of gas chromatograph with help of a neat, labeled, schematic block diagram. Write a note on classification, principle, factors influencing the separation and criteria to perform the Gas chromatography.
- a) Draw an UV-Visible double beam spectrophotometer with a neat, labeled, block diagram and explain its each component operational mode.
b) Why a double beam spectrophotometer gives more precise, reliable and reproducible results in comparison to a single beam spectrophotometer?
c) What are the factors influencing the absorption of radiant energy?
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- Write notes on: (10 x 6 =60)
- Give a brief and comprehensive account of application of conductometry?
- State Beer-Lambert’s law. Derive it Write its-application, deviation and limitation.
- Discuss the underlying principle and interference of atomic absorption spectrometry.
- Name the detectors used in HPLC. Discuss anyone of them.
- Differentiate validation and calibration. Give a brief account of validation of analytical methods.
- Explain the theory and procedure of DSC.
- Discuss the fundamental theory of mass spectroscopy.
What are the different ions produced in mass spectrum? - What is quenching? What are different type of quenching?
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Write a note on factors affecting the fluorescence intensity. - Explain the various method of sample handling in IR-Spectroscopy.
- Give a brief account of :
a) Indicator electrode, b) Polarizable electrode, c) Spectrophotometric titration.
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