[LH 4266] AUGUST 2015 Sub. Code: 4266
B.PHARM. DEGREE EXAMINATION
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THIRD YEAR
(Common to Regulations 2004 — IV year candidates)
PAPER VI - PHARMACEUTICAL BIOTECHNOLOGY
Q.P. Code: 564266
Time : Three Hours Maximum : 100 marks
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Answer All Questions
- Essay: (2x20=40)
- Explain the methods of production of interferon by rDNA technology.
- Differentiate moist heat and dry heat sterilization
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- Define fermentation. Explain the fermentative production of Penicillin.
- Explain the production of insulin by genetic engineering technology.
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II. Short notes : (8 x5=40)
- Bacterial growth curve.
- Gram’s staining technique.
- Microbiological assay of antibiotics.
- Production of single cell protein.
- BCG Vaccine.
- Bioprocess of citric acid
- Techniques in immobilization of enzyme.
- Production and application of monoclonal antibodies.
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III. Short answers: (10x 2=20)
- Bacteriophage.
- Hepatitis -B.
- Tyndallization.
- Plasmid
- Culture Media.
- Examples of Motile and non motile bacteria.
- Define Immobilization.
- Define Toxoid with examples.
- Types of flagella.
- Restriction endonucleases.
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