THIRD YEAR B.PHARM. EXAMINATION
(Common to Regulations 2004 — IV year candidates)
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PHARMACEUTICAL BIOTECHNOLOGY
Time: Three hours Maximum: 100 marks
I. Essay: (2x20=40)
- a) Differentiate between Gram positive and Gram negative cell wall with a neat labeled diagram.
b) Define virus. Explain bacterial growth curve. - a) What are Mono Clonal antibodies? Explain its methods of preparation and its application.
b) Explain the design and construction of the Fermenter.
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II. Short notes: (8x5=40)
- Hot air oven
- Preparation of diphtheria toxoid
- Factors affecting disinfection
- Bioprocessing of ethanol
- Hyper sensitivity reaction
- Microbiological assay of vitamin B12
- Regulation of gene expression
- Production of Hepatitis B vaccine by rDNA technology
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III. Short Answers: (10x2=20)
- Define immunity
- Examples of killed bacterial vaccine
- Define Biosensor
- Synchronous culture
- Classify Disinfectant
- Use of Western Blot test
- Examples of Motile bacteria
- Define Viable count
- Define Sterilization
- Define Vectors
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