Download B.Pharm 3rd Year 2016 February 564266 Pharmaceutical Biotechnology Question Paper

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(L1 4266) FEBRUARY 2016 Sub. Code: 4266

THIRD YEAR B.PHARM. EXAMINATION

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(Common to Regulations 2004 — IV year candidates)

PAPER VI - PHARMACEUTICAL BIOTECHNOLOGY

Q.P. Code: 564266

Time: Three hours Maximum: 100 Marks

I. Essay: (2x20=40)

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1. a) Explain building blocks of Protein Synthesis in a Bacterial cell.
   b) Immuno blotting techniques of ELISA and Western blot.
2. a) Classify different methods of Sterilization. Explain merits and demerits of physical methods of Sterilization.
   b) Add a note of Sterilization by filtration.

II. Short notes: (8x5=40)

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1. Transduction.
2. Isolation and identification of Bacteria.
3. Describe the structure of DNA with neat diagram.
4. Fermentative production of Citric acid.
5. How an antibiotic can be assayed by Biological Method?

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6. Biosensors.
7. Bio assay of Vitamins.
8. Bacterial counting techniques.

III. Short answers: (10x2=20)

1. Mean generation time.

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2. Incineration.
3. Fimbria and Pili.
4. DNA Gyrase & DNA Ligase.
5. Nucleotide.
6. Write the three letters of Initiating Codons and Terminating Codons.

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7. Sparge
8. Live Bacterial vaccines.
9. Restriction endonucleases.
10. Down streaming process.

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