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Download MBBS Ion Selective Electrodes Lecture PPT

Download MBBS (Bachelor of Medicine and Bachelor of Surgery) Latest Ion Selective Electrodes Lecture PPT

This post was last modified on 30 November 2021


KEY WORD AND DEFINITIONS

Electrode: A conductor through which an electrical

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current enters or leaves a nonmetallic portion of a circuit.

Indicator electrode: Used in potentiometry that produces

a potential representative of the species being measured.

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Reference electrode: Is an electrode at which no

appropriate current is allowed to flow and which is used

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to observe or control the potential of the indicator or

working electrode, respectively.
Potentiometry: An electrochemical process where the

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potential difference is measured between an indicator

electrode and reference electrode when no current is

allowed to flow in the electrochemical cell.

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WORKING PRINCIPLE

Membrane potentials are caused by the permeability of

certain types of membranes to selected anions or cations.

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Such membranes are used to fabricate ion-selective

electrodes that selectively interact with a single ionic

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species.

The potential produced at membrane-sample solution

interface is proportional to the logarithm of the ionic

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activity or concentration of the ion in question

Measurements with ISEs are simple, often rapid,

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nondestructive, and applicable to a wide range of
concentrations.

The ion-selective membrane is the "heart" of an ISE

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as it controls the selectivity of the electrode.

Ion-selective membranes typically consist of glass,

crystalline, polymeric materials.

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Ionic-selective electrodes are widely

used clinically for the measurement of

pH, PCO2 and electrolytes in whole

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blood, serum, plasma and urine.