Download SGBAU BSc 2019 Summer 2nd Sem Biotechnology Microbiology Question Paper

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B.Sc. (Part-I) Semester—II Examination

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Note :—

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1. (1) All questions are compulsory.
2. (2) Draw well labelled diagrams wherever necessary.

1. (A) Fill in the blanks : 2
   1. (i) In optical microscope __________ is used as source of illumination.
   2. (ii) In bacterial classification name of the family is suffixed with __________.
   3. (iii) In microscopy, TEM stands for __________.

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   4. (iv) __________ first explained theory of host parasite relationship for pathogenic microorganisms.
2. (B) Choose correct option :— 2
   1. (i) Mantoux test is used for
      1. (a) Measles
      2. (b) Hepatitis
      3. (c) Tuberculosis

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      4. (d) Polio
   2. (ii) Saccharomyces is
      1. (a) Saccharide
      2. (b) Saccharin
      3. (c) Yeast

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      4. (d) Mold
   3. (iii) __________ is the counter stain in Gram Staining.
      1. (a) Crystal violet
      2. (b) Iodine
      3. (c) Safranin

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      4. (d) Methylene blue
   4. (iv) In microbiology laboratory, bacterial culture is preserved in
      1. (a) Autoclave
      2. (b) Hot air oven
      3. (c) Refrigerator

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      4. (d) Incubator
3. (C) Answer in ONE sentence each :— 4
   1. (i) Photocell
   2. (ii) Phagocytosis

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   3. (iii) Sterilization
   4. (iv) Lithotrophs

1. Explain —
   1. (a) Objectives in compound microscope. 4
   2. (b) Membrane filter. 4

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   3. (c) Gaseous chemosterilization. 4
   OR
   1. (e) Simple staining. 4
   2. (f) Typical bacterial cell. 4
2. 1. (a) Classify microbes on the basis of energy source. 4

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   2. (b) Differentiate between gracilicutes and firmicutes 4
   3. (c) Describe importance of methanogens. 4
   OR
   1. (d) Describe teichoic acids of bacterial cell wall. 4
   2. (e) Differentiate between flagella of Gram positive and Gram negative bacteria. 4
   3. (f) Explain halophiles. 4

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3. Explain fixation of atmospheric nitrogen by following microorganisms :
   1. (a) Azotobacter 4
   2. (b) Rhizobium 4
   3. (c) Cyanobacteria 4
   OR
   1. (d) Describe symbiotic association. 4

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   2. (e) Define antibiosis. Explain with suitable example. 4
   3. (f) Explain ATP generation steps in electron transport chain. 4
4. Explain the role of following microbes as agricultural biofertilizers :
   1. (a) Rhizobium 4
   2. (b) Azotobacter 4

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   3. (c) PSB. 4
   OR Explain the importance of following industrially important microorganisms :
   1. (d) Aspergillus 4
   2. (e) Penicillium 4
   3. (f) Spirulina 4

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5. Describe in detail specific host defence mechanism. 12 OR Describe in detail mycoplasma as pathogenic organism. 12
6. Describe in detail working and applications of UV-VIS spectrophotometer. 12 OR Discuss biotechnologically important radioactive isotopes. 12

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