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B.Sc. (Part—II) Semester—IV Examination
4S : BIOCHEMISTRY
(Enzymology)
Time : Three Hours] [Maximum Marks : 80
Note : — (1) All questions are compulsory.
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(2) Draw well labelled diagrams wherever necessary.1.
(A) Fill in the blanks : 2
(i) A Coenzyme required in transamination is _______.
(ii) In Line Weaver-Burk plot, the x-intercept represents _______.
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(iii) In competitive inhibition, the inhibitor competes with _______.
(iv) _______ ions are cofactors for Hexokinase.
B) Choose correct alternative :
(i) The following Coenzyme takes part in hydrogen transfer reactions :
(a) Tetrahydrofolate (b) Coenzyme A
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(c) Coenzyme Q (d) Biotin
(ii) Lactate dehydrogenase is a :
(a) Monomer (b) Dimer
(c) Tetramer (d) Hexamer
(iii) Different isoenzymes of an enzyme have the same :
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(a) Amino acid sequence (b) Michaelis Constant
(c) Catalytic activity (d) All of the above
(iv) Allosteric inhibition is also known as :
(a) Competitive inhibition (b) Non competitive inhibition
(c) Feedback inhibition (d) None of the above
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C) Answer in ONE sentence :— 4
(i) Marker enzyme
(ii) Km
(iii) Holoenzyme
(iv) Immobilized enzyme.
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2. Explain:
(a) Metallo Enzymes 4
(b) Nomenclature of enzymes. 4
(c) Isoenzymes. 4
OR
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(p) Multienzyme complexes.
(q) Four digit classification of enzymes. 4
(r) Active site. 4
3. (a) Explain any one test for homogeneity. 4
(b) Describe the effect of pH on enzyme activity. 4
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(c) Explain Km and Vmax with the help of Line Weaver Burk Plot. 4
OR
(p) Explain in short Ping Pong mechanism. 4
(q) Describe any one method used for isolation of enzyme. 4
(r) Describe enzyme assay briefly. 4
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4. Describe in detail competitive inhibition with the help of double reciprocal plot. 12
OR
Discuss zero order and first order reaction Kinetics. 12
5. Describe the role of :
(a) THF 4
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(b) FAD and FMN. 4
(c) Pyridoxal phosphate. 4
OR
Explain :
(p) Allosteric inhibition. 4
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(q) Metal activated enzymes. 4
(r) Coenzyme Q. 4
6. Describe in short Lock and Key hypothesis and induced fit model. 12
OR
Give a brief account of acid-base catalysis and covalent catalysis. 12
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7. Describe :
(a) Industrial application of immobilized enzymes. 4
(b) Production of glucose-fructose syrup from sucrose. 4
(c) Use of Lactose in dairy industry. 4
OR
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(p) Use of proteases in food. 4
(q) Medical applications of enzymes. 4
(r) Production of glucose from starch 4
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