Download RGUHS (Rajiv Gandhi University of Health Sciences) MBBS (Bachelor of Medicine and Bachelor of Surgery) 2023 February 1025 Biochemistry Paper II Rs 4 Previous Question Paper
Rajiv Gandhi University of Health Sciences, Karnataka
MBBS Phase ? I (CBME) Degree Examination - 16-Feb-2023
Time: Three Hours
Max. Marks: 100 Marks
BIOCHEMISTRY ? PAPER II (RS-4)
Q.P. CODE: 1025
(QP contains three pages)
Your answers should be specific to the questions asked
Draw neat, labeled diagrams wherever necessary
LONG ESSAYS
2 x 10 = 20 Marks
1.
A 10 year old boy was brought to the hospital for self-multilating behaviour and delayed
development. His serum uric acid level was 15 mg/dl. A provisional diagnosis of Lesch Nyhan
syndrome was made.
a) Write the biological reference interval for serum uric acid level.
b) Name the enzyme defect and the pathway affected in the above disorder.
c) Which tissues are dependent on this pathway?
d) Enumerate the other causes of hyperuricemia
e) What is the role of allopurinol in the treatment of hyperuricemia?
2.
Describe the steps of DNA replication in prokaryotes. List the difference between
prokaryotic and eukaryotic DNA replication.
SHORT ESSAYS
8 x 5 = 40 Marks
3. a) A 15 year old tribal boy was admitted with complaints of fatigue, recurrent fever and pain in
arms and legs. His peripheral blood smear shows sickled erythrocytes and a hemoglobin
concentration of 6 gm%.
a) Name the molecular defect in this disorder.
b) Explain the mechanism of sickling.
c) What is the cause of anemia in this condition?
4. d) An intravenous drug user was found to have developed jaundice. Laboratory investigations
revealed hepatitis B infection. His total serum bilirubin was 10.2 mg/dl.
a) What type of jaundice is seen in above case?
b) Explain van den Bergh test and write its interpretation in the above case.
5.
Describe the steps in this polymerase chain reaction (PCR). Name the type of PCR used in
the diagnosis of Covid 19.
6.
Name any three products derived from tyrosine. Describe the metabolic pathway leading to
the formation of any one of the product from tyrosine.
7.
Draw the structure of immunoglobulin G and label it. Enumerate its functions.
8.
What is lipid peroxidation? Explain the role of antioxidants in scavenging reactive oxygen
species.
9.
Write the steps in the formation of creatine and glutathione from glycine.
10. Describe the glomerular function tests.
Page 1 of 3
QP CODE : 1025
Rajiv Gandhi University of Health Sciences, Karnataka
SHORT ANSWERS
10 x 3 = 30 Marks
11. Name three tumor markers and mention their significance.
12. What is the enzyme defect in maple syrup urine disease? What are its characteristic
features?
13. List the adrenal function tests. Mention one disorder associated with abnormal adrenal
function.
14. Enlist three biologically important peptides and write their functions.
15. What are xenobiotics? Write two detoxification reactions by conjugation.
16. Name three biologically important nucleotides and mention their importance.
17. Mention three advantages of automation in clinical biochemistry laboratory.
18. Enlist three post-translational modifications with suitable examples.
19. Mention normal albumin/globulin (A/G) ratio. Write two conditions where A/G ratio is
altered.
20. What is restriction fragment length polymorphism (RFLP). Mention two clinical applications
of RFLP.
Multiple Choice Questions
10 x 1 = 10 Marks
21 i) All the following are glucogenic amino acids EXCEPT
A. Valine
B. Phenylalanine
C. Glycine
D. Leucine
21 ii
) N3 of purine ring is donated by
A. Asparate
B. Glycine
C. Glutamine
D. Ammonia
21 ii
i) Which of the following enzymes is responsible for immortality of cancer cells?
A. Telomerase
B. RNA polymerase I
C. DNA polymerase
D. Hel
icase
21 iv)
In which of the following conditions is serum direct bilirubin elevated
A. Physiological jaundice in the newborn
B. Crigler ? Najjar syndrome
C. Gilbert's syndrome
D. Gall stones
21 v)
Enzyme defect in acute intermittent porphyria is
A. ALA synthase
B. ALA dehydratase
C. PBG deaminase
D. Uropor
phyrinogen decarboxylase
Page 2 of 3
QP CODE : 1025
Rajiv Gandhi University of Health Sciences, Karnataka
22 i) Closeness of a result to the true value is termed as
A. Precision
B. Accuracy
C. Sensitivity
D. Spe
cificity
22
ii) Reference interval for pH of urine is
A. 3.5 to 4.5
B. 5.5 to 6.5
C. 7.5 to 8.5
D. 9.5 to 10.5
22 iii) Southern blotting technique is used for detection of
A. DNA
B. RNA
C. Protein
D. Lipoprotein
22 iv)
Which of the following is an example for acute phase protein?
A. Prothrombin
B. C-reactive protein
C. Lipoprotein A
D. Thyroxine binding globulin (TBG)
22 v)
Epidermal growth factor (EGF) is produced by
A. Platelets
B. Monocytes
C. Endothelial cells
D. Fibroblasts
******
Page 3 of 3
This post was last modified on 02 December 2024