Download GTU B.Pharma 2020 Winter 6th Sem 2260002 Pharmaceutical Microbiology And Biotechnology Ii Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Pharma (Bachelor of Pharmacy) 2020 Winter 6th Sem 2260002 Pharmaceutical Microbiology And Biotechnology Ii Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B.Pharm. - SEMESTER?VI ? EXAMINATION ? WINTER -2020
Subject Code:2260002
Date: 05/01/2021
Subject Name: Pharmaceutical microbiology & Biotechnology-II
Time: 2:00 PM TO 4:00 PM
Total Marks: 54
Instructions:


1. Attempt any THREE questions from Q-1 to Q-6.
2. Q.7 is compulsory to attempt.
3. Make suitable assumptions wherever necessary.
4. Figures to the right indicate full marks.

Q.1 (a) Define mutation. Discuss the importance of Frame shift mutation and point
06
mutation.

(b) Write a note on physical, chemical and biological type of mutagens.
05

(c) Discuss the Gradient Plate Method.
05



Q.2 (a) Write a note on transformation, conjugation and transduction.
06

(b) Write a note on Gene cloning and their applications
05

(c) Explain applications of HAT media in production of monoclonal antibodies.
05



Q.3 (a) Discuss the result interpretation of sterility testing.
06

(b) Comments: (i) Sub-culturing is required in sterility testing of oily injections.
05
(ii) Positive control is also known as sterility control.

(c) Write a note on cylindrical plate method.
05



Q.4 (a) Discuss general production,standardization and storage of vaccines.
06

(b) Differentiate: (i)Vaccines and sera
05

(c) Comments: (i) Second line defence is not a specific defence.
05
(ii)TAB is simple monovalent vaccine.



Q.5 (a) Classify different types of vaccines and write a note on vaccines for hepatitis
06
and AIDS.

(b) Write a note on Blotting techniques.
05

(c) Write a note on Immunoglobulins.
05



Q. 6 (a) Write a note on processing and storage of whole human blood and dried human
06
plasma.

(b) Discuss media and media sterilization in Fermentation technique.
05

(c) Draw diagram of ideal fermentor. What are the roles of different part of
05
fermentor.



Q.7 (a) Give the Name of organisms required for production of: (i) Alcohol (ii)
06
Vitamin B12 (iii) Riboflavin (iv) Tetracyclines (v) Streptomycins (vi) Citric
acid.
OR

(a) Compare and Contrast: Fermentation technique for production of penicillin G
06
and Penicillin V.
OR

(a) Discuss the different parameter needs to be adjusted for fermentation technique. 06
***************

---