Download GTU B.Pharma 2018 Winter 8th Sem 2280015 Genetic Engineering And Gene Therapy Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Pharma (Bachelor of Pharmacy) 2018 Winter 8th Sem 2280015 Genetic Engineering And Gene Therapy Previous Question Paper

Seat No.: ________ Enrolment No.______________

GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM ? SEMESTER ? 8- EXAMINATION ?WINTER - 2018

Subject Code: 2280015
Date: 28/11/2018
Subject Name: Genetic engineering and gene therapy
Time: 02:30 PM TO 05:30 PM
Total Marks: 80
Instructions:

1. Attempt any five questions.

2. Make Suitable assumptions wherever necessary.

3. Figures to the right indicate full marks.

Q.1 (a) Explain Gene Cloning with neat and labelled diagram.
06

(b) Write a note on applications of genetic engineering.
05

(c) What is central dogma of life? Explain the process of translation with diagram.
05



Q.2 (a) What are vectors in genetic engineering? Enumerate the types of vectors.
06
Explain plasmid PBR322 as a vector in detail with diagram.

(b) What is RNA? Write a note on types of RNAs.
05

(c) What is PCR explain the cycle and write applications of PCR.
05



Q.3 (a) What do you mean by Antisense oligo nucleotide therapy?
06

(b) What are monoclonal antibodies? How they are produced? Write the
05
applications of Monoclonal Anti bodies.

(c) What is blotting? Explain southern blotting with suitable diagram.
05



Q.4 (a) What are transgenic plants? How they are produces? Write the applications of
06
transgenic plants.

(b) What is Humulin? Explain the production using recombinant DNA technology.
05

(c) What do you mean by gene therapy? Explain the types with suitable example.
05



Q.5 (a) Explain DNA delivery methods in detail.
06

(b) Write a note on Quantification of Nucleic acids.
05

(c) Explain in detail about transgenic animals.
05



Q. 6 (a) Write in detail about DNA sequencing techniques. Explain any one technique
06
using suitable diagram.

(b) Write a note on transformation with diagram.
05

(c) Explain about DNA vaccine in detail.
05



Q.7 (a) Explain the application of gene therapy in Hematopoietic disorders.
06

(b) What are restriction enzymes? Explain the types and applications in detail.
05

(c) Explain about bacteriophage with suitable diagram.
05
***************

---