Download GTU B.Pharma 2018 Summer 8th Sem 2280015 Genetic Engineering And Gene Therapy Question Paper

Download GTU (Gujarat Technological University) B.Pharma (Bachelor of Pharmacy) 2018 Summer 8th Sem 2280015 Genetic Engineering And Gene Therapy Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharmacy - SEMESTER? VIII ? EXAMINATION ? SUMMER-2018
Subject Code:2280015
Date: 09/05/2018
Subject Name: Genetic Engineering and Gene Therapy
Time:10:30am to 01:30pm
Total Marks: 80

Instructions:


1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Write applications of genetic engineering.
06

(b) Explain gene organization in cell.
05

(c) Explain synthesis of cDNA.
05



Q.2 (a) Explain the methods used for isolation of RNA.
06

(b) Explain about DNA modifying enzymes.
05

(c) Write a note on transformation.
05



Q.3 (a) Enumerate the types of vectors. Explain any one vector in detail with diagram.
06

(b) Write in detail about gene cloning with neat labeled diagram.
05

(c) Write short note on Transgenic plants.
05



Q.4 (a) What is PCR? Explain applications of PCR in detail.
06

(b) Write a short note on handling and quantification of nucleic acids.
05

(c) Enumerate different of DNA sequencing. Explain any one method of
05
DNA sequencing in detail with suitable diagram.



Q.5 (a) What are monoclonal antibodies? How they are produced using the techniques
06
of genetic engineering?

(b) Explain PCR cycle in detail.
05

(c) Explain about DNA vaccines against Cancer.
05



Q. 6 (a) Write a note on Automatic DNA sequence.
06

(b) Write about r-DNA technology in treatments of medical conditions.
05

(c) Write short note on gene therapy for hematopoietic disorders,
05



Q.7 (a) Write a note on antisense- oligonucleotide therapy.
06

(b) Write short note on gene therapy for Neurological Diseases.
05

(c) Discuss blotting techniques in detail.
05
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