Download MBBS Biochemistry PPT 1 L2 Fatty Acids Lecture Notes

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Study Of Derived Lipids

Study Of Fatty Acids
FATTY ACIDS( FAs)

Class- Derived Lipids



BASIC COMPONENT

OF LIPID FORMS

What are Fatty Acids?
Fatty Acids Are Derived Lipids

? Fatty acids are of Class Derived Lipids:

?Since Fatty acids are Hydrolytic

products of Simple and Compound

Lipids.

Fatty Acids (FA)

? Fatty Acids (FA) are relatively or

potential y related to various Lipid

structures.

?Simple Lipids
?Compound Lipids
?Derivatives of Lipids


Structure And Chemical Nature

Of Fatty Acids

Chemical Structure Of Fatty Acids
Fatty acid Structures Has

Varied Hydrocarbon Chains

? The Hydrocarbon chain of

each Fatty acid is of varying

chain length (C2 - C26).

Human Body Fatty Acid From C2-C26

S.No Fatty Acid Name Fatty Acid Structure has

Carbon atoms

1

Acetic Acid

C2

2

Propionic Acid

C3

3

Butyric Acid

C4

4

Valeric Acid

C5

5

Palmitic

C16

6

Stearic

C18

7

Oleic

C18
S.No

Fatty Acid Name

Fatty Acid Structure

8

Linoleic Acid

C18

9

Arachidic Acid

C20

10

Arachidonic Acid

C20

11

Behenic acid

C22

12

Lignoceric acid

C24

13

Cerotic acid

C26

? Fatty acid structure have two

ends:

? Carboxylic group(-COOH) at one end (Delta end

denoted as /Alpha end )

? Methyl group (-CH3) at another end (Omega

end denoted as )


Carboxylic Acid Functional Group Of

Fatty Acid

Definition of Fatty acids
Fatty Acids are Defined as:

? Fatty acids are chemical y Organic acids

? With Aliphatic Hydrocarbon chain (of varying

length C2 to C26) with Mono terminal

Carboxylic acid group as functional group.



Different Forms Of

Fatty acids In Body
Free Fatty acid /Unesterified Fatty acid

? Fatty acid who has free Carboxylic group

? Fatty acid not reacted and linked to an

Alcohol by an Ester bond.

Esterified Fatty acid/Bound form of Fatty Acid

?Fatty acid has no free Carboxylic

group

?Fatty acid is linked to an Alcohol

with an Ester bond.
Classification of Fatty acids

Biomedical y Important Fatty Acids

Based On Six Different Modes:

? Classification of FAs Based on Six Modes:

1. Total number of Carbon atoms in a Fatty acid structure

2. Hydrocarbon chain length of Fatty acid

3. Bonds present in Fatty acid

4. Nutritional requirement of Fatty acid

5. Chemical Nature and Structure of Fatty acids

6. Geometric Isomerism of UFAs
Fatty acids Based on

Total Number of Carbon atoms

?Even numbered Carbon Atom Fatty

acids (2,4,6,8,16,18,20 etc)

?Odd numbered Carbon Atom Fatty

acids (3,5,7,---)
? Most natural y occurring /human body Fatty

acids are even carbon numbered FAs.


? Since biosynthesis of Fatty acids uses 2

Carbon units Acetyl-CoA (C2).

? Examples of Even Carbon Numbered

Fatty acids:

? Butyric Acid (C4)
? Palmitic Acid (C16)
? Stearic Acid (C18)
? Oleic Acid (C18) (Most Common)
? Linoleic acid (C18)
? Linolenic Acid (C18)
? Arachidic acid (C20)
? Arachidonic acid (C20)
? Odd Carbon numbered Fatty acids are less related to

human body

? Example of Odd carbon Fatty acid associated to

human body

?Propionic Acid ( 3C)

Fatty acids Based on

Nature and Number of Bonds present



? Saturated Fatty acids(SFAs)

? Fatty acids having single bonds in hydrocarbon

chain structure.

? Examples:

? Acetic acid (C2)

? Butyric acid (C4)

? Palmitic acid (C16)

? Stearic acid (C18)

? Arachidic acid(C20)

? Unsaturated Fatty acids (UFAs)

? Fatty acids having double bonds in its structure.

? Types of UFAs:

? Monounsaturated Fatty acids (MUFAs)

? Polyunsaturated Fatty acids (PUFAs)


? Human body have no Enzyme system to introduce

double bond beyond Carbon atom 10 in the

hydrocarbon chain.

? Hence PUFAs are not biosynthesized
in human beings.
? Monounsaturated Fatty Acids(MUFAs):

? MUFAs have one double bond in a fatty acid structure

? Examples of MUFAs :

? Palmitoleic acid (C16:1;9) (7)
? Oleic acid (C18:1;9)(9)
? Erucic acid (C22:1;9)(9)

? Poly Unsaturated Fatty Acids (PUFAs):

? UFAs with two or more double bonds in
the structure are termed as PUFAs.

? Examples Of PUFAS:

? Linoleic(18:2;9,12) (6)
? Linolenic(18:3;9,12,15) (3)
? Arachidonic(20:4;5,8,11,14) (6)
? Timnodonic (20:5;5,8,11,14,17) (3)
? Cervonic/Docosa Hexaenoic

acid(DHA)(22:6;4,7,10,13,16,19) (3)
? Remember Unsaturated Fatty acids

? Double bonds are:

? Weaker /unstable bonds.

? Get easily cleavable/metabolized

?More the degree of Unsaturation in Fatty

acids.

?More is the unstability of Fatty acids.


? Saturated Fatty acids structures are

Straight.

? Unsaturated Fatty acids structures are

bent (Kink).
? Saturated FAs: with straight structures are

tightly packed together.

? Unsaturated FAs: with bent structures are

not compact and has no tight packing.

? More the degree of unsaturation in

FA/More double bonds in FA

structure

? More is the bent of Fatty acid

structure.



Fatty acids Based on the Nutritional

Requirement
Nutritionally Essential Fatty

acids

?Nutritional y Essential Fatty acids:

?Fatty acids not biosynthesized in

human body and indispensable

through nutrition/diet are termed as

Essential Fatty acids.

?PUFAS are nutritional y essential

Fatty acids.
Examples of Essential Fatty Acids/PUFAs:

?Linoleic

?Linolenic



?Arachidonic acids

?Timnodonic and

?Cervonic

Nutritional y Non Essential Fatty acids
? Nutritional y Non essential Fatty acids:

? Fatty acids which are biosynthesized in the body

and are nutritional y non essential Fatty acids.

? Saturated Fatty acids and MUFAs are non essential

Fatty acids.

Examples Of Non Essential Fatty Acids

? Palmitic
? Stearic
? Oleic acid
Based on Geometric Isomerism of

Unsaturated Fatty acids

? Cis Fatty Acids:

The Groups around double bond of Unsaturated FAs

are on same side.
? Examples:

? Cis Oleic acid (rich in Olive oil)
? Palmitoleic acid


? Trans Fatty Acids :

? The groups around double bond of UFAs are on

opposite side

? Example :

? Elaidic acid /Trans Oleic acid (Hydrogenated

Fats )
Types Of Fatty acids Based on

Hydrocarbon chain length

? Short Chain Fatty acids (2-6 Hydrocarbon Chain length)

? Examples:

? Acetic acid (C2)
? Propionic acid (C3)
? Butyric acid (C4)
? Valeric acid (C5)
? Caproic acid (C6)
? Medium Chain Fatty acids (8-14 Carbon length)
? Examples:

? Caprylic acid (C8)
? Capric acid (C10)
? Lauric acid (C12)
? Myristic acid (C14)

? Long Chain Fatty acids ( 16-20 Carbon length)
? Examples:

? Palmitic acid (C16)
? Palmitoleic acid (C16)
? Stearic acid (C18 )
? Oleic acid (C18)
? Linoleic acid (C18)
? Linolenic acid (C18)
? Arichidic acid (C20)
? Arachidonic acid /ETA(C20)
? Timnodonic acid/EPA (C20)
? Very Long Chain Fatty Acids (C22 onwards )

? Examples:

? Behenic acid/Docosanoic (C22)

? Erucic acid/Docosa 13 Enoic (C22)

? Clupanodonic/Docosapentaenoic acid (DPA) (C22)

? Cervonic acid/DocosaHexaenoic (DHA) (C22)

? Lignoceric acid /Tetracosanoic (C24)

? Nervonic /Tetracosaenoic (C24)

? Cerotic acid/Hexacosanoic (C26)

Fatty acids Based on

Chemical Nature and Structure


?Aliphatic Fatty acids:

Straight Hydrocarbon chain

? Examples:

?Palmitic acid (C16)
?Stearic acid (C18)

? Branched Chain Fatty acids:

? Possess Branched chains

? Examples:

?Isovaleric (C5)

?Phytanic acid (Butter , dairy products)


? Cyclic Fatty acids :

? Contains Ring structure

? Examples:

? Chaulmoogric acid

(Used for Leprosy treatment in olden days)

? Hydnocarpic acid

? Hydroxy Fatty acids:

? Contain Hydroxyl Groups

? Examples:

? Cerebronic acid (C24)/

2-HydroxyTetracosanoic acid

? Ricinoleic acid(C18) (Castor oil)
Naming And Numbering

Of Fatty Acids

? Every Fatty acids has a:

? Common Name
? Systematic Name


? Most of the Fatty acids are known by their

common names.(Since easy to use)

? Systematic names of Fatty acids are limited

in use. (Since not easy to use)

Remember

? Long chain Fatty acids are

also termed as Acyl chains.
vThe systematic names of Saturated Fatty

acids are named by adding suffix `anoic'.

v Example : Palmitic acid- C16/ Hexadecanoic

acid

? The systematic names of Unsaturated Fatty

acids are named by suffix `enoic'.

? Example: Oleic acid- C18/ Octadecaenoic

acid
S.N Common Name Systematic Name

1

Palmitic Acid

Hexadecanoic Acid

2

Stearic Acid

Octadecanoic Acid

3

Oleic acid

Octadecaenoic acid

4

Linoleic Acid

Octadecadienoic acid

5

Linolenic Acid

Octadecatrienoic acid

6

Arachidonic acid Eicosatetraenoic acid

Numbering Of Fatty Acids
? Numbering of Carbon atoms of

Fatty acids is done from :

?Both ends of Fatty acids-

? end/ end
? end

Numbering Of Fatty acid From

Carboxyl/ end ( end)

? From Carboxyl Group end( end ) :
? Carboxylic acid group of Fatty acid is

numbered as C1

? C2 is next adjacent Carbon atom ,
? C3 and so onn..........


? Carbon atom is next to the functional

group ?COOH of a Fatty acid.

? Next to Carbon is , , , and so onn.

? Carbon atoms from Methyl(?CH3)

group /non polar end() of a fatty

acid are numbered as 1,2,3

and so onn.
Nomenclature Of Fatty

acids

? FA Nomenclature is Based On

? Chain length/Total Number of Carbon atoms in a FA.

? Count Number of Carbon atoms in FA

? Number and Position of Double bonds

? Position of double bond from Carboxyl/Delta end

? Position of double bond from Methyl/Omega
Short Hand Representations

of Fatty acids

? Short Hand Representations

of Fatty acids:

?Palmitic Acid (16:0)
?Palmitoleic acid (16:1;9)
?\

? First digit stands for total number of carbon atoms

in the fatty acid.

? Second digit designates number of double bonds.
? Third digit onwards indicates the position of

double bonds.


Fatty-acid Nomenclature

? Named according to chain length

? C18

Fatty-acid Nomenclature

? Named according to the number of

double bonds

? C18:0

Common name:

Stearic acid




Fatty-acid Nomenclature

? Named according to the number of

double bonds

? C18:1

Common name:

Oleic acid

Fatty-acid Nomenclature

? Named according to the number of

double bonds

? C18:2

Common name:

Linoleic acid


Fatty-acid Nomenclature

? Named according to the number of

double bonds

? C18:3

Common name:

Linolenic acid

Omega System Nomenclature

? Named according to the

location of the first double bond from the non-carboxyl Methyl

end (count from the Methyl end /Omega end )


Omega Fatty-acid Nomenclature

Omega 9 or n?9 fatty acid

Omega 6 or n?6 fatty acid

Omega 3 or n?3 fatty acid

?Stearic acid (18:0)
?Oleic acid (18:1;9)
?Linoleic acid (18:2;9,12)
?Linolenic acid (18:3;9,12,15)
?Arachidonic acid (20:4;5,8,11,14)
? A Fatty acid may also be designated as :
? Linoleic acid (18C;9,12)
? Linolenic acid (18C;9,12,15)
? indicates from COOH end.
? 9,12,15 are double bond positions from delta

end.

Short Hand Presentation of FA

14:0 Myristic acid
16:0 Palmitic acid
18:0 Stearic acid
18:1 cis D9 Oleic acid (9)
18:2 cisD9,12 Linoleic acid (6)

18:3 cisD9,12,15 a-Linolenic acid (3)

20:4 cisD5,8,11,14 Arachidonic acid (6)

20:5 cisD5,8,11,14,17 Eicosapentaenoic acid (3 )

22:5 CisD7,10,13,16,19 Docosapentaenoic acid (3 )
Important Properties Of Fatty Acids

Properties Of Fatty Acids

? Physical Properties

? Chemical Reactions




Physical Properties Of Fatty Acids

1. Solubility
2. Melting Point

Solubility Of Fatty Acids Depends

Upon
Factors Responsible For Solubility Of

Fatty Acids

1. Hydrocarbon chain length

2. Degree of Unsaturation- Number of Double Bonds

3. Hydrophobicity/Polarity of Fatty acids

4. Polarity of Solvents

? Smal hydrocarbon chain length are less

hydrophobic and more soluble

? Long Chain FA and VLCFA more hydrophobic are

very less soluble

? Solubility of Fatty acids decreases with increase in

Fatty acid hydrocarbon chain length.

? Double bonds increases solubility
Melting Point of Fatty Acids

Factors Responsible For

Melting Points Of Fatty Acids

1. Hydrocarbon chain length

2. Nature of Bonds

3. Degree of Unsaturation/Number of double bonds
Fatty Acids With

Decreased Melting Points

? Short and Unsaturated Fatty

acids has low melting point

? More degree of unsaturation low

is melting point of FAS

Melting Points

? Affected by chain length

?Longer chain = higher melting temp

Fatty acid: C12:0

C14:0

C16:0

C18:0

C20:0

Melting point: 44?C

58?C

63?C

72?C

77?C
Melting Points

? Affected by number of double bonds

?More saturated = higher melting temp

Fatty acid:

C18:0

C18:1

C18:2

C18:3

Melting point:

72?C

16?C

?5?C

?11?C
Fatty Acids With

Increased Melting Points

? Long and Saturated Fatty acids are

has high melting point.

? Less degree of Unsaturation more is

melting point of Fatty acids

? Thus melting point of Fatty acids(FAs):

?Increases with increase in chain

length of FAs.

?Decreases with decrease in chain

length of FAs.

?Increases with low unsaturation of

FAs

?Decreases with more unsaturation of

Fatty acids


Structures and Melting Points of Saturated Fatty Acids
Chemical Reactions Of Fatty Acids

Types Of Chemical Reactions

Of Fatty acids

Reactions due to

Carboxyl group of Fatty acids:

? Esterification/Esterified forms of Lipids

? Saponification/Soap Formation
Reactions Associated to

Double bonds of Fatty acids:

? Halogenation/Addition of Halogens around double

bond

? Hydrogenation/Transform to UFAs to SFAs

Significance Of Halogenation

?Halogenation of fatty

acids is an index of

assessing the degree of

unsaturation
? Iodine Number is a process of

Halogenation which checks the

content of SFA and PUFAs of Fats

and Oils.

? SFA has zero Iodine number.

? PUFAs has high Iodine number.

Hydrogenation Of Fatty acids

Alters Geometric Isomerism Of

Unsaturated Fatty acids

Transforms Natural Cis Form to Trans Form

Increases Shelf life of PUFAs
Al -Cis Fatty acids

Good for Health

? Human body contain Enzyme system to

metabolize Cis form of Fatty acids.

? Cis forms when ingested through food are

easily metabolized and does not retain in the

body.

? Hence All ?Cis forms are good for health and no

risk of Atherosclerosis and CVD.

? Al Cis form of fatty acids are unstable and

easily metabolizable.

? More content of Trans Fatty acids are found

in processed/Refined foods viz:

?Hydrogenated Oils (Vanaspati Dalda)
?Ghee
?Margarine
?Bakery products /Fast foods
?Deeply Fried recipes in Oils which are

prepared in repeatedly heated oils.
? Trans fatty acids increases risk of

:

?Atherosclerosis
?Cardio Vascular disorders:

?Ischemia
?Myocardial Infarction

?Stroke(Brain attack)

Message

Learnt, Understood And To Be Implemented

For Good Fatty acid metabolism and Significant Health

? Eat natural Cis forms of Fatty acids
? Avoid Hydrogenated Trans Fatty

acids

? Eat home made food
? Avoid Processed/Junk Foods
PUFAs And Omega Fatty Acids

Types Of Omega Fatty acids

?In Nutrition and Clinical

practice

?3 Fatty acids

?6 Fatty acids

?7 Fatty acids

?9 Fatty acids
Omega Fatty Acids

Omega 3 Fas

Omega 6 Fas

Omega 7 Fas

Omega 9 Fas

PUFAs

PUFAs

MUFAs

MUFAs

Linolenic

Linoleic

Palmitoleic Oleic ?

C18-(ODTA)

C18- (ODDA) C16-(HDA) C18(ODA)

Timnodonic

Erucic ?

C20-(EPA)

Arachidonic

C22(DA)

Cervonic

C20-(ETA)

Nervonic

C22-(DHA)

C24-(TA)

Clupanodonic

C22-(DPA)

Examples of 3 Fatty acids

? Linolenic (18:3;9,12,15) (3)

? Timnodonic/Ecosapentaenoic Acid /EPA

(20:5;5,8,11,14,17)(3)

? Clupanodonic acid/(Docosa Pentaenoic Acid): (DPA)

(C22:5;7,10,13,16,19 )(3)

? Cervonic/Docosa Hexaenoic Acid

(DHA)(22:6;4,7,10,13,16,19)(3)
Rich sources of dietary Omega and nutritional

essential PUFAS are:

?Vegetable Oils
?Green Leaves, Algae
?Fish and Fish oils
?Flax Seeds

Sources,Distribution,Composition Of

Fatty Acids In Human Body
Sources Of Fatty Acids To Human Body

? Exogenous Sources- Dietary Food Items

? Endogenous Biosynthesis- From Free Excess
Glucose in Liver

Forms of Dietary Fatty Acids To Be Ingested

Natural Forms Of Fatty Acids

? Fatty acids in nature mostly presently in

? Esterified form of FAs? (TAG,PL,CE)
? Even Numbered Carbon
? Unsaturated- PUFAs/Omega 3 and 6
? Cis forms
Contents Of Fatty acids Sources Of Oils

Highest Content of MUFA Olive Oil , Mustard Oil

Highest content of PUFA Safflower, Sunflower,

Flax seed Oil

Highest content of SFA

Coconut Oil

Oils Rich In

Oils rich in

Oils rich in

SFAs

MUFAs

PUFAs

Coconut Oil

Olive Oil (75%)

Flax seeds/

Linseed Oil

Palm Oil

Sunflower Oil (85%)

Soya /Safflower Oil

Butter

Ground nut /

Almond Oil

Pea nut Oil

Animal Fat

Almond Oil

Rice Bran

Sesame Oil

Walnuts Oil

Beef Fat (Tal ow Fat) 50%

Corn Oil

Lard (Pork Fat) 40%

Marine Fish



Carbons

Double bonds

Abbreviation

Source

Fatty Acids

Acetic

2

0

2:0

bacterial metabolism

Propionic

3

0

3:0

bacterial metabolism

Butyric

4

0

4:0

butterfat

Caproic

6

0

6:0

butterfat

Caprylic

8

0

8:0

coconut oil

Capric

10

0

10:0

coconut oil

Lauric

12

0

12:0

coconut oil

Myristic

14

0

14:0

palm kernel oil

Palmitic

16

0

16:0

palm oil

Palmitoleic

16

1

16:1

animal fats

Stearic

18

0

18:0

animal fats

Oleic

18

1

18:1

olive oil

Linoleic

18

2

18:2

grape seed oil

Linolenic

18

3

18:3

flaxseed (linseed) oil

Arachidonic

20

4

20:4

peanut oil, fish oil
Fatty acid Composition

of Human Body

Fatty acid

Percentage

Oleic acid

50% (MUFA)

Palmitic acid

35% (SFA)

Lionleic acid

10% (PUFA)

Stearic acid

5% (SFA)

? Thus most abundant Fatty acids

present in human Lipids are:

?Oleic acid (50%)
?Palmitic acid(35%)
Ideal Requirement

Of Fatty Acids To Human Body

? It is ideal to consume ratio of:

?1 : 1 : 1

? SFA MUFA PUFAs

? respectively from the diet to maintain good

health.
? Naturally there is no single oil which has

all 3 types of fatty acids in ideal

proportion.

? Hence it is always advisable to mix a

combination of oils and consume.

Transportation Of Fatty Acids In

Human Body
? Bound form /Esterified

Forms Of Fatty acids are

Transported through

various Lipoproteins.

Fatty acids Transportation In body

? More than 90% of the fatty acids found in

plasma are in the form of Fatty acid esters.

? Fatty acids Esters/Esterifed form of Fatty

acids exist as:

? Triacylglycerol
? Cholesteryl esters
? Phospholipids
? Unesterified/Free Fatty acids (FFA)

are very less amount in body.

? Long Chain FFA are transported in

the blood circulation in association

with Albumin.

Functions Of Fatty Acids

1. Secondary Source Of Energy
2. Components Of Biomembranes
3. PUFA (Arachidonic Acid) Precursor for Eicosanoid

Biosynthesis

4. Esterification of Cholesterol and its Excretion
5. PUFAs build and protect Brain and Heart
6. PUFAs prevents early ageing, prolongs Clotting

time.
? PUFAs of membrane play role

in:(Less compact)

?Membrane fluidity
?Selective permeability

Functions Of

PUFAS /Omega 3, and 6 FAs

? Components of cel biomembranes

? More associated to Human brain and Heart

? Involve in Growth ,development and

functioning of Brain
? Omega Fatty acids Reduces risk of Heart disease:

?Reduces Platelet aggregation by stimulating

Prostaglandins and Prostacyclin's .

?Reduces blood clotting and Thrombus

formation by Lowering the production of

Thromboxane .

? Omega 3 Fatty acids have pleiotropic effects
(more than on effect):

?Cardio protective effect

? Lowers Blood pressure
? Anti-Inflammatory
? Anti-Atherogenic
? Anti-Thrombotic
? PUFAs Lowers Risk Of Atherosclerosis

? Since double bonds of PUFAs are unstable and

easily cleavable.

? PUFAs get easily metabolized and do not get

accumulated in the blood arteries and capillaries.

? Thus PUFAs have low risk of Atherosclerosis and

Cardio vascular disorders.

? Fish (rich in Omega 3 Fatty acids) Eaters has

Healthy Brain and Heart

? Brain development with an efficient

nervous function.

? Protected from Heart attacks.
? Deficiency of Essential Fatty acids :

? Affects every cel ,organ and system

?Growth retardation
?Problems with reproduction
?Skin lesions
?Kidney and Liver disorders
?Brain disorders/Behavioral disorders.

Deficiency Of PUFAs/ Omega 3,6

Fatty acids

? Deficit of omega fatty acids affect the normal

growth ,development and functioning of brain.

? Persons may suffer from mental il ness like:

?Depression
?Attention deficit
?Dementia=Alzheimer's Disease
? Deficiency of Omega 3 Fatty acids :

?Alters the cell membrane structure.
?Increases the risk of

? Heart attack
? Cancer
? Rheumatoid Arthritis

Phrynoderma /Toad Skin is due

to PUFA deficiency.
? Phrynoderma /Toad Skin Symptoms

? The skin becomes dry with lesions
(Scaly Dermatitis).
? Presence of horny erruptions on the posterior and lateral

parts of limbs, back and Buttock.

? Loss of hair
? Poor wound healing
? Acanthosis and Hyperkeratosis

? Deficiency of PUFAs lower:

?Oxidative Phosphorylation-ATP generation

?Fibrinolytic Activities
Fatty Acids At Glance

Name of Biomolecule Fatty acids

Class

Derived Lipids

Structural Features

Organic acids ,Hydrocarbon Chain (C2-

26) Terminal Mono Carboxylic Acid

Sources of FAs to

From Exogenous and Endogenous

body
Distribution in Body FAs mostly in esterified form,

Associated with Simple and

Compound Lipids. Distributed in all

tissues.

Functional aspects

Energy, Biomembrane components

Interrelationships

Fatty acids associated to other form

of Simple and Compound Lipids

This post was last modified on 05 April 2022