Download PTU (Punjab Technical University) BSc Agriculture 7th Semester 74825 INTRODUCTION TO MOLECULAR BIOTECHNOLOGY Last 10 Years 2020, 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011 and 2010 Previous Question Papers.
1 | M-74825 (S2)-2259
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc. (Agriculture) (2014 & Onwards) (Sem.?7)
INTRODUCTION TO MOLECULAR BIOTECHNOLOGY
Subject Code : BSAG-702
M.Code : 74825
Time : 3 Hrs. Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.
SECTION-A
Q1. Write short notes on :
a) Operons
b) Histones
c) Transformation
d) Gene tagging
e) Mapping population
f) Hot start PCR
g) Marker assisted gene pyramiding
h) Genome
i) Repetitive DNA
j) Synonymous and nonsynonymous SNPs
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1 | M-74825 (S2)-2259
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc. (Agriculture) (2014 & Onwards) (Sem.?7)
INTRODUCTION TO MOLECULAR BIOTECHNOLOGY
Subject Code : BSAG-702
M.Code : 74825
Time : 3 Hrs. Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.
SECTION-A
Q1. Write short notes on :
a) Operons
b) Histones
c) Transformation
d) Gene tagging
e) Mapping population
f) Hot start PCR
g) Marker assisted gene pyramiding
h) Genome
i) Repetitive DNA
j) Synonymous and nonsynonymous SNPs
2 | M-74825 (S2)-2259
SECTION-B
Q2. a) What is the basis of polymorphism of RAPD markers?
b) Write any two features of the primers used for development of RAPD markers.
c) Name two dominant and co-dominant markers each.
Q3. Write a short note on selection of transformed cells in a cloning experiment.
Q4. a) Who first discovered restriction endonucleases?
b) Define Cohesive ends and examples of enzymes producing cohesive ends.
c) What are the major differences between genome organizations among eukaryotes and
prokaryotes?
Q5. a) Define Gene Cloning and competent cells.
b) Describe the principle and applications of Marker Assisted Selection.
Q6. What are the functions of various important components of a PCR reaction? How can you
determine the annealing temperature of a primer?
SECTION-C
Q7. Define genomic library. Write in details the steps for the construction of genomic libraries
and their applications.
Q8. a) Discuss the principle of Sanger sequencing technique.
b) Define NextGen sequencing techniques. Name any two techniques. What are various
applications for which sequencing data could be utilized?
Q9. What is the principle of Southern hybridization? Write in detail its procedure and
applications.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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This post was last modified on 07 December 2019