Download PTU B-Tech Bio Technology 2020 Dec 6th Sem 71074 Enzymology And Enzyme Technology Question Paper

Download PTU (I.K.Gujral Punjab Technical University (IKGPTU)) B-Tech (Bachelor of Technology) BT-Bio Technology 2020 December 6th Sem 71074 Enzymology And Enzyme Technology Previous Question Paper

Roll No.
Total No. of Pages : 02
Total No. of Questions : 18
B.Tech.(Biotechnology) (2012 Onwards) (Sem.?6)
ENZYMOLOGY AND ENZYME TECHNOLOGY
Subject Code : BTBT-603
M.Code : 71074
Time : 3 Hrs. Max. Marks : 60
INST RUCT ION TO CANDIDAT ES :
1 .
SECT ION-A is COMPULSORY cons is ting of TEN questions carrying TWO marks
each.
2 .
SECT ION-B c ontains F IVE questions c arrying FIVE marks eac h and s tud ents
have to atte mpt any FOUR q ues tions.
3 .
SECT ION-C contains THREE questions carrying T EN marks e ach and s tudents
have to atte mpt any T WO questio ns.
SECTION-A
Answer the following questions briefly :
1.
What is the difference between specific activity and turn over number of an enzyme?
2.
What is the difference between biological catalyst and chemical catalyst?
3.
Explain the difference between biocatalysis and biotransformation by giving suitable
examples.
4.
What are allosteric enzymes?
5.
Define Isoenzymes. Give two examples.
6.
What is PEGylation and why is it carried out? Give relevant examples.
7.
What is competitive enzyme inhibition?
8.
What is the importance for Km and Vmax values?
9.
What is the need of a double reciprocal plot?
10. Differentiate between coenzymes and cofactors with examples.
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SECTION-B
11. Although trypsin, chymotrypsin and elastase, all are serine proteases how do they have
different specificity for protease action?
12. Write short notes (approx. 500-600 words) on any one of the following :
a) Competitive Inhibition
b) Enzyme Inhibitors
c) Lineweaver- Burke Plot and its importance
13. Give an account of application of enzymes in medicine and therapeutics.
14. Discuss the role of crystallization in the process of enzyme purification at industrial scale .
15. Give the merits and demerits of enzyme immobilization.
SECTION-C
16. Explain the Ping-Pong and Ternary Complex mechanisms of multi-substrate enzyme
reactions. Give suitable example of each mechanism.
17. Entrapment is yet another process of enzyme immobilization. What kinds of matrices are
used for the entrapment of the biocatalyst? Which is the best matrix for enzyme entrapment
for industrial applications?
18. What are technical enzymes? Briefly discuss the role of proteases in the detergent industry.
NOTE : Disclosure of identity by writing mobile number or making passing request on any
page of Answer sheet will lead to UMC case against the Student.
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