Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Summer 7th Sem New 2170407 Biochemical Engineering I Previous Question Paper
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VII(NEW) EXAMINATION ? SUMMER 2019
Subject Code:2170407 Date:16/05/2019
Subject Name:Biochemical Engineering-I
Time:02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) What is role of bioprocess engineer in an industry? 03
(b) Explain difference between steady state and equillibrium. 04
(c) A continuous process is set up for treatment of wastewater. Each day,
10
5
kg cellulose and 10
3
kg bacteria enter in the feed stream, while 10
4
kg cellulose and 1.5 x 10
4
kg bacteria leave in the effluent. The rate of
cellulose digestion by the bacteria is 7 x10
4
kg d
-1
. The rate of bacterial
growth is 2 x 10
4
kg d
-l
; the rate of cell death by lysis is 5 x 10
2
kg d
-1
.
Write balances for cellulose and bacteria in the system.
07
Q.2 (a) Explain the terms intensive and extensive properties. 03
(b) Explain the term Heat of combustion. 04
(c) Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest
can be considered solids. Beet molasses containing 50% sucrose, 1%
invert sugars, 18% water and the remainder solids is mixed with corn-
steep liquor in a mixing tank. Water is added to produce adiluted sugar
mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor
and 45 kg molasses are fed into the tank.
(a) How much water is required?
(b) What is the concentration of sucrose in the final mixture?
07
OR
(c) Water at 25
o
C enters an open heating tank at a rate of 10 kg h
-
1. Liquid
water leaves the tank at 88
o
C at a rate of 9 kg h
-1
; 1 kg h
-1
water vapour
is lost from the system through evaporation. At steady state, what is the
rate of heat input to the system?
07
Q.3 (a) What is Reynold?s number? What is its significance? 03
(b) What is difference between radial flow impeller and axial flow
impeller?
04
(c) What is viscosity? Explain working of Coaxial-cylinder Rotary
Viscometer with diagram.
07
OR
Q.3 (a) What is role of diffusion in bioprocessing? 03
(b) Enlist different methods for determination of kLa. Explain any one in
detail.
04
(c) Explain the steps for transfer of gas from bubble to a cell with a neat
diagram.
07
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1
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VII(NEW) EXAMINATION ? SUMMER 2019
Subject Code:2170407 Date:16/05/2019
Subject Name:Biochemical Engineering-I
Time:02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) What is role of bioprocess engineer in an industry? 03
(b) Explain difference between steady state and equillibrium. 04
(c) A continuous process is set up for treatment of wastewater. Each day,
10
5
kg cellulose and 10
3
kg bacteria enter in the feed stream, while 10
4
kg cellulose and 1.5 x 10
4
kg bacteria leave in the effluent. The rate of
cellulose digestion by the bacteria is 7 x10
4
kg d
-1
. The rate of bacterial
growth is 2 x 10
4
kg d
-l
; the rate of cell death by lysis is 5 x 10
2
kg d
-1
.
Write balances for cellulose and bacteria in the system.
07
Q.2 (a) Explain the terms intensive and extensive properties. 03
(b) Explain the term Heat of combustion. 04
(c) Corn-steep liquor contains 2.5 % invert sugars and 50% water; the rest
can be considered solids. Beet molasses containing 50% sucrose, 1%
invert sugars, 18% water and the remainder solids is mixed with corn-
steep liquor in a mixing tank. Water is added to produce adiluted sugar
mixture containing 2% (w/w) invert sugars. 125 kg corn-steep liquor
and 45 kg molasses are fed into the tank.
(a) How much water is required?
(b) What is the concentration of sucrose in the final mixture?
07
OR
(c) Water at 25
o
C enters an open heating tank at a rate of 10 kg h
-
1. Liquid
water leaves the tank at 88
o
C at a rate of 9 kg h
-1
; 1 kg h
-1
water vapour
is lost from the system through evaporation. At steady state, what is the
rate of heat input to the system?
07
Q.3 (a) What is Reynold?s number? What is its significance? 03
(b) What is difference between radial flow impeller and axial flow
impeller?
04
(c) What is viscosity? Explain working of Coaxial-cylinder Rotary
Viscometer with diagram.
07
OR
Q.3 (a) What is role of diffusion in bioprocessing? 03
(b) Enlist different methods for determination of kLa. Explain any one in
detail.
04
(c) Explain the steps for transfer of gas from bubble to a cell with a neat
diagram.
07
2
Q.4 (a) What do you mean by total rate, volumetric rate and specific rate? 03
(b) Derive the equation for kinetics of cell death. 04
(c) Glucose isomerase is used extensively in the USA for production of
high-fructose syrup. The reaction is:
glucose ? fructose.
?H
o
rxn for this reaction is 5.73 kJ gmol
-l
; ?S
o
rxn is 0.0176 kJ gmol
-l
K
-
1
.
(a) Calculate the equilibrium constants at 50
o
Cand 75
o
C
(b) A company aims to develop a sweeter mixture of sugars, i.e. one
with a higher concentration of fructose. Consideringequilibrium only,
would it be more desirable to operate the reaction at 50
o
C or 75
o
C?
07
OR
Q.4 (a) What do you mean by theoretical and observed yields? 03
(b) Compare the equations of Lineweaver-Burk Plot, Eadie-Hofstee Plot
and Langmuir Plot.
04
(c) Explain Michaelis-Menten Kinetics 07
Q.5 (a) What is difference between chemostat and turbidostat? 03
(b) Explain kinetics of Substrate Uptake in the Absence of Product
Formation.
04
(c) Explain in detail typical batch growth curve and also relate it to specific
growth rate.
07
OR
Q.5 (a) Give applications of trickle bed, packed bed and fluidized bed reactors? 03
(b) What considerations must be given for fermenter designing for
inoculation and sampling control?
04
(c) Explain the process of batch heat sterilization of liquids in detail. 07
*************
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This post was last modified on 20 February 2020