Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2018 Winter 7th Sem New 2170407 Biochemical Engineering I Previous Question Paper
1
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VII (NEW) EXAMINATION ? WINTER 2018
Subject Code: 2170407 Date: 26/11/2018
Subject Name: Biochemical Engineering-I
Time: 10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) Define the terms: Respiratory quotient, Yield, Growth rate limiting reaction. 03
(b) Discuss the sterilization of air for the fermentation operation.
Give an account of each with suitable explanations.
04
(c) A fermenter containing microbial culture at 30
0
C is used for production of
microbial insecticide with its 20-litre capacity. KLa is to be determined. Air flow
is shut off for few minutes and DO level drops the air supply is then
reconnected. When steady state is established, the DO tension is 77% air
saturation.
The following results are obtained.
T1 T2
Time 5 15
Oxyge
n
tension
50 66
Where, CAL = final steady DO concentration and C AL = DO
Concentration. (a) Estimate KLa. (b) An error is made determining
steady state oxygen level, which instead of 77% is taken as 69%, what
is the percentage error in KLa resulting from this error in CAL.
07
Q.2 (a) How do the analogy exists between mass and heat transfer? 03
(b) Discuss the basic steps for designing of a bioreactor. 04
(c) The fungus Aureobasidium pullulans is used to produce extracellular
polysaccharides by fermentation of sucrose. After 120h fermentation, the
following measurements of shear stress and shear rate were made with a rotating
cylinder viscometer.
Shear Stress dyn
cm
-2
Shear Rate
s
-1
44.1 10.2
235.3 170
357.1 340
457.1 510
636.8 1020
[1] Plot the rheogram for this fluid.
[2] Determine the appropriate Non Newtonian parameters.
[3] find the apparent viscosity at the shear rates of ; 15 s
-1
and 200 s
-1
.
07
OR
(c) A bacterium converts ethanol to acetic acid under aerobic conditions. A
continuous fermentation process for vinegar production is proposed using non-
viable bacterial cells immobilized on the surface of gelatin beads. The
production target is 2 kg h
-1
acetic acid; however the maximum acetic acid
07
www.FirstRanker.com www.FirstRanker.com
www.FirstRanker.com
FirstRanker.com - FirstRanker's Choice
www.FirstRanker.com
1
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VII (NEW) EXAMINATION ? WINTER 2018
Subject Code: 2170407 Date: 26/11/2018
Subject Name: Biochemical Engineering-I
Time: 10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) Define the terms: Respiratory quotient, Yield, Growth rate limiting reaction. 03
(b) Discuss the sterilization of air for the fermentation operation.
Give an account of each with suitable explanations.
04
(c) A fermenter containing microbial culture at 30
0
C is used for production of
microbial insecticide with its 20-litre capacity. KLa is to be determined. Air flow
is shut off for few minutes and DO level drops the air supply is then
reconnected. When steady state is established, the DO tension is 77% air
saturation.
The following results are obtained.
T1 T2
Time 5 15
Oxyge
n
tension
50 66
Where, CAL = final steady DO concentration and C AL = DO
Concentration. (a) Estimate KLa. (b) An error is made determining
steady state oxygen level, which instead of 77% is taken as 69%, what
is the percentage error in KLa resulting from this error in CAL.
07
Q.2 (a) How do the analogy exists between mass and heat transfer? 03
(b) Discuss the basic steps for designing of a bioreactor. 04
(c) The fungus Aureobasidium pullulans is used to produce extracellular
polysaccharides by fermentation of sucrose. After 120h fermentation, the
following measurements of shear stress and shear rate were made with a rotating
cylinder viscometer.
Shear Stress dyn
cm
-2
Shear Rate
s
-1
44.1 10.2
235.3 170
357.1 340
457.1 510
636.8 1020
[1] Plot the rheogram for this fluid.
[2] Determine the appropriate Non Newtonian parameters.
[3] find the apparent viscosity at the shear rates of ; 15 s
-1
and 200 s
-1
.
07
OR
(c) A bacterium converts ethanol to acetic acid under aerobic conditions. A
continuous fermentation process for vinegar production is proposed using non-
viable bacterial cells immobilized on the surface of gelatin beads. The
production target is 2 kg h
-1
acetic acid; however the maximum acetic acid
07
www.FirstRanker.com www.FirstRanker.com
www.FirstRanker.com
www.FirstRanker.com
2
concentration tolerated by the cells is 12%. Air is pumped into the fermenter at
a rate of 200 mmol h
-1
.
(a) What minimum amount of ethanol is required?
(b) What minimum amount of water must be used to dilute the
ethanol to avoid acid inhibition?
Q.3 (a) Derive the equations for Eddie-Hofstee plot and Lineweaver ?Burke plot. 03
(b) Enlist the energy concerns from heat transfer point of view for a bioreactor. 04
(c) Write about Non- Newtonian fluids with suitable equations. Explain each of
them for its stress and strain relation analogous to the law of viscosity.
07
OR
Q.3 (a) Discuss the dynamic method to determine K L a. 03
(b) Enlist the enzymes which are medical importance. 04
(c) Discuss the practical considerations for bioreactor constructions. 07
Q.4 (a) Write a note on: Orifice and Nozzle spargers 03
(b) Discuss aseptic operation by giving schematic to show operating procedures. 04
(c) Explain the concept of air lift bioreactor. Compare it with fluidized bed
bioreactor.
07
OR
Q.4 (a) What is the importance of modeling? Where and how does it help in
fermentation plants?
03
(b) Explain the measurement of DO concentrations. 04
(c) Justify the statement: ?Chemostate culture evaluates the kinetic and yield
parameters.?
07
Q.5 (a) Derive equation to narrate death kinetics of cell culture. 03
(b) How cells grow? Which factors affect? 04
(c) Define bioprocess engineering. Explain the role of biotechnology in that. 07
OR
Q.5 (a) What are the effects of conditions on Enzyme reaction rate? 03
(b) Write a note on: Assessing mixing effectiveness 04
(c) Write the transfer of oxygen from gas bubbles to cell. 07
*************
www.FirstRanker.com www.FirstRanker.com
www.FirstRanker.com
FirstRanker.com - FirstRanker's Choice
This post was last modified on 20 February 2020