Download PTU B.Tech 2020 March Biotechnology 5th Sem BTBT 503 Genetic Engineering Question Paper

Download PTU (I.K. Gujral Punjab Technical University Jalandhar (IKGPTU) ) BE/BTech Bio-Technology (Biotechnology Engineering) 2020 March 5th Sem BTBT 503 Genetic Engineering Previous Question Paper

1 | M-70504 (S2)-152
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Tech.(BT) (2012 to 2017) (Sem.?5)
GENETIC ENGINEERING
Subject Code : BTBT-503
M.Code : 70504
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
1. Answer briefly :
a) Differentiate between BamHI and BamHI Methyltransferase.
b) Distinguish between E. coli DNA Polymerase I and Klenow fragment.
c) Differentiate between plasmid and cosmid vector.
d) ?E. coli is a preferred host in molecular cloning?. Justify the statement.
e) Elucidate the principle of Southern blot analysis.
f) State briefly the biological importance of DNA-protein interactions.
g) What are the ingredients required to set up a PCR?
h) What is DNA microarray technique?
i) How do you demonstrate the expression of a recombinant protein in E. coli?
j) Write a brief note on in vitro transcription.


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1 | M-70504 (S2)-152
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Tech.(BT) (2012 to 2017) (Sem.?5)
GENETIC ENGINEERING
Subject Code : BTBT-503
M.Code : 70504
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
1. Answer briefly :
a) Differentiate between BamHI and BamHI Methyltransferase.
b) Distinguish between E. coli DNA Polymerase I and Klenow fragment.
c) Differentiate between plasmid and cosmid vector.
d) ?E. coli is a preferred host in molecular cloning?. Justify the statement.
e) Elucidate the principle of Southern blot analysis.
f) State briefly the biological importance of DNA-protein interactions.
g) What are the ingredients required to set up a PCR?
h) What is DNA microarray technique?
i) How do you demonstrate the expression of a recombinant protein in E. coli?
j) Write a brief note on in vitro transcription.


2 | M-70504 (S2)-152
SECTION-B
2. State the attributes and applications of Kinases and Ligases with examples.
3. How do you construct a mouse liver cDNA library?
4. What do mean by gene-specific probes? How do you radiolabel them?
5. Write briefly any four applications of PCR.
6. Write the principle and applications of site-directed mutagenesis.

SECTION-C
7. Illustrate the genetic features of the yeast and mammalian expression vectors.
8. Write in detail the principle and steps of oligonucleotide synthesis.
9. Write precise notes on the following :
YAC vector, Western blot analysis.






NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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