Download OU (Osmania University) B.Sc First Year (1st Year) Microbiology Question Bank Question Paper
B.Sc.IYearPracticalExamination2019-2020
Stihjcct:NIICROBI()I.()CY(NewSyllabus)
Paper:I(IntroductoryMicrobiology)
QuestionBank
|'l?imc:2IIouhshrlax.Ivlnrks:50
IMajorl-lxperimcnt(Questions(lX20-20Nlarlas)
l.Alightcoinpound111lCl'O.'\'t?l)|)t?._stagerniciotnctci"andocularmicrontctcrtireprovidetoyou.
Calibratethemicroscopeforitsmeasurctnentinl0X(lowpower)and45X[lzighpower).
Reportthecalibratedwtluesineachmagnification.
2.Acalibratedmicroscopearrangedwithtactilartnicrtxmetcrisprovidedtoyou.Preparea
slideofitiicrosctwtiiitutiiect(bacteriait:tang-a]spores)andmeasurethesize01'objectwith
tln:helpotkntiibratteitoculartnicioirtctcrin10wpowerandhighpower.Reporttheresult
antidcitiunstizttcyourOhSCfVdllOTl.
(Note:Internalextiminerisrequiredtopro-calibratethemicroscopeandgivethe
calibratedvaluesintxmsultatiotiwithco-exztmittcr).
3.Abacterialpurt:eultureisprovidedtoyou.Preparethesntcarofsameatndstainby
differential(Grams)stainingrrzethtitl.Observethemicroscopiccharacteristicsofstained
cultureantireportthemorphology,turrangcritu-rttandstainingnature.Demonstrateyour
ooscrvzition.
4.StainthegvuitbacterialL'Lt.llll'(?byendosptvrcstainingandreportyourtibscrvtationby
demonslrat..~'-_>_yourobsorvctiIicld.
u.
Stainthegivenbacterialcultureby?agcllarstainingandreportyourobservationby
demonstratingyourobservedfield.'
6.Anactivelygrowingculture
providedtoyou.Prepareasmearandstainbycattsulc
stainingtnethozlandreportyourobservation.
ll.Minorl-ZxperintontQuestions(IXI0-"10Marks]
7Demnitstrzttetheprcpztration-:>f?agzti'platesandslants.Explaintheirapplications
R.Demonstratestreak
spreadplatetechniquefor"isolatingpuregulrureg,
9Demonsarepourplatetechnique.Fxpliuinitsadvantagesanddisadvantages
l0.Demonstrateseriall0Folddilutionmethodandextiluin.
ll.Abatcterietlcultuneis].\1'~JVl(.lC(ltoyou.Identifythetypeoftnicroorgaitismbyits
hiocltetnicalruaclit?.5.
l2.Demortstrattcthegtenbacterialmotilitybyhatngingdropmethod.\?t-'i"?.tetlu:principle
.DBenn;"iciif?ia"
f,n
'maIILBOS
Dgtcgiftlllcrobiolvtllt
Osmaniauniv?'$?Y~?*'?'
FACELTYOFSCIENCE
B.Sc.IYearPracticalExamination2019-2020
Stihjcct:NIICROBI()I.()CY(NewSyllabus)
Paper:I(IntroductoryMicrobiology)
QuestionBank
|'l?imc:2IIouhshrlax.Ivlnrks:50
IMajorl-lxperimcnt(Questions(lX20-20Nlarlas)
l.Alightcoinpound111lCl'O.'\'t?l)|)t?._stagerniciotnctci"andocularmicrontctcrtireprovidetoyou.
Calibratethemicroscopeforitsmeasurctnentinl0X(lowpower)and45X[lzighpower).
Reportthecalibratedwtluesineachmagnification.
2.Acalibratedmicroscopearrangedwithtactilartnicrtxmetcrisprovidedtoyou.Preparea
slideofitiicrosctwtiiitutiiect(bacteriait:tang-a]spores)andmeasurethesize01'objectwith
tln:helpotkntiibratteitoculartnicioirtctcrin10wpowerandhighpower.Reporttheresult
antidcitiunstizttcyourOhSCfVdllOTl.
(Note:Internalextiminerisrequiredtopro-calibratethemicroscopeandgivethe
calibratedvaluesintxmsultatiotiwithco-exztmittcr).
3.Abacterialpurt:eultureisprovidedtoyou.Preparethesntcarofsameatndstainby
differential(Grams)stainingrrzethtitl.Observethemicroscopiccharacteristicsofstained
cultureantireportthemorphology,turrangcritu-rttandstainingnature.Demonstrateyour
ooscrvzition.
4.StainthegvuitbacterialL'Lt.llll'(?byendosptvrcstainingandreportyourtibscrvtationby
demonslrat..~'-_>_yourobsorvctiIicld.
u.
Stainthegivenbacterialcultureby?agcllarstainingandreportyourobservationby
demonstratingyourobservedfield.'
6.Anactivelygrowingculture
providedtoyou.Prepareasmearandstainbycattsulc
stainingtnethozlandreportyourobservation.
ll.Minorl-ZxperintontQuestions(IXI0-"10Marks]
7Demnitstrzttetheprcpztration-:>f?agzti'platesandslants.Explaintheirapplications
R.Demonstratestreak
spreadplatetechniquefor"isolatingpuregulrureg,
9Demonsarepourplatetechnique.Fxpliuinitsadvantagesanddisadvantages
l0.Demonstrateseriall0Folddilutionmethodandextiluin.
ll.Abatcterietlcultuneis].\1'~JVl(.lC(ltoyou.Identifythetypeoftnicroorgaitismbyits
hiocltetnicalruaclit?.5.
l2.Demortstrattcthegtenbacterialmotilitybyhatngingdropmethod.\?t-'i"?.tetlu:principle
.DBenn;"iciif?ia"
f,n
'maIILBOS
Dgtcgiftlllcrobiolvtllt
Osmaniauniv?'$?Y~?*'?'
ll.Specimenforspotting
I3
.Microscope
.4.
lnoculaliuvnIooyzfrlccdlc
l5.Nutrientagarplate
l6.
IJFJIOIJPJ
~"?bl~J?
25.
'.Nnstoc
.Spirulina
u-I
?I
.5?
Nulriculagarskull
.AgardccpIubes
.Autoclave
.Ilotairoven
Filtration;aeppurmus
.Streakplaic-
.Spreadplane
".Grampositiveluanilli
.Grzunpositivecocci
(iramnegativcbacilli
.ekpcrgillus
.Rhizopus
.Sacclulrontyccs
.Pencillium
EleclrorlMicroscopicinwgeof'I?.\'I\/'
HlecucnMicrosoupicimageoFH1\-?
.[minklcsl
T.Simmon?soilral:slant
Ill.Record&Viva
(5X3=1Sfvlarks)
(5Marks)
FACELTYOFSCIENCE
B.Sc.IYearPracticalExamination2019-2020
Stihjcct:NIICROBI()I.()CY(NewSyllabus)
Paper:I(IntroductoryMicrobiology)
QuestionBank
|'l?imc:2IIouhshrlax.Ivlnrks:50
IMajorl-lxperimcnt(Questions(lX20-20Nlarlas)
l.Alightcoinpound111lCl'O.'\'t?l)|)t?._stagerniciotnctci"andocularmicrontctcrtireprovidetoyou.
Calibratethemicroscopeforitsmeasurctnentinl0X(lowpower)and45X[lzighpower).
Reportthecalibratedwtluesineachmagnification.
2.Acalibratedmicroscopearrangedwithtactilartnicrtxmetcrisprovidedtoyou.Preparea
slideofitiicrosctwtiiitutiiect(bacteriait:tang-a]spores)andmeasurethesize01'objectwith
tln:helpotkntiibratteitoculartnicioirtctcrin10wpowerandhighpower.Reporttheresult
antidcitiunstizttcyourOhSCfVdllOTl.
(Note:Internalextiminerisrequiredtopro-calibratethemicroscopeandgivethe
calibratedvaluesintxmsultatiotiwithco-exztmittcr).
3.Abacterialpurt:eultureisprovidedtoyou.Preparethesntcarofsameatndstainby
differential(Grams)stainingrrzethtitl.Observethemicroscopiccharacteristicsofstained
cultureantireportthemorphology,turrangcritu-rttandstainingnature.Demonstrateyour
ooscrvzition.
4.StainthegvuitbacterialL'Lt.llll'(?byendosptvrcstainingandreportyourtibscrvtationby
demonslrat..~'-_>_yourobsorvctiIicld.
u.
Stainthegivenbacterialcultureby?agcllarstainingandreportyourobservationby
demonstratingyourobservedfield.'
6.Anactivelygrowingculture
providedtoyou.Prepareasmearandstainbycattsulc
stainingtnethozlandreportyourobservation.
ll.Minorl-ZxperintontQuestions(IXI0-"10Marks]
7Demnitstrzttetheprcpztration-:>f?agzti'platesandslants.Explaintheirapplications
R.Demonstratestreak
spreadplatetechniquefor"isolatingpuregulrureg,
9Demonsarepourplatetechnique.Fxpliuinitsadvantagesanddisadvantages
l0.Demonstrateseriall0Folddilutionmethodandextiluin.
ll.Abatcterietlcultuneis].\1'~JVl(.lC(ltoyou.Identifythetypeoftnicroorgaitismbyits
hiocltetnicalruaclit?.5.
l2.Demortstrattcthegtenbacterialmotilitybyhatngingdropmethod.\?t-'i"?.tetlu:principle
.DBenn;"iciif?ia"
f,n
'maIILBOS
Dgtcgiftlllcrobiolvtllt
Osmaniauniv?'$?Y~?*'?'
ll.Specimenforspotting
I3
.Microscope
.4.
lnoculaliuvnIooyzfrlccdlc
l5.Nutrientagarplate
l6.
IJFJIOIJPJ
~"?bl~J?
25.
'.Nnstoc
.Spirulina
u-I
?I
.5?
Nulriculagarskull
.AgardccpIubes
.Autoclave
.Ilotairoven
Filtration;aeppurmus
.Streakplaic-
.Spreadplane
".Grampositiveluanilli
.Grzunpositivecocci
(iramnegativcbacilli
.ekpcrgillus
.Rhizopus
.Sacclulrontyccs
.Pencillium
EleclrorlMicroscopicinwgeof'I?.\'I\/'
HlecucnMicrosoupicimageoFH1\-?
.[minklcsl
T.Simmon?soilral:slant
Ill.Record&Viva
(5X3=1Sfvlarks)
(5Marks)
-
--..?--_-
FACULTY0FSCIENCE
B.Sc.IYearPracticalExamination2019-2020
Subject:NIICROBIOLOGY(NewSyllabus)
PaperzI(IntroductoryMicrobiology)
N?adclQucstionPaper
lime:3HOIIISNIQZQIIIZIfkSI50
I..\/']?jUl'l-ixpcrilnerltquestion(IXZO-?ZOMarks}
ll.MinorL-lxpcrirrlcntquestion(1XI0-1llh/Izlrks)
III.Spotters(5X3?'|Slvlarks)
IA)
B)
c;
D)
.E)
I\-'.Record8:viva(5Marks)
?FLAP-WIm
ChairmanBoS
DepLoIMicrobiology
OsmaniaUniversity)-,4,
This post was last modified on 21 November 2019