Download OU (Osmania University) B.Sc Fourth Year (4th Year) Microbiology QB Question Paper
B.Sc.IVSemester(Practical)BTxatuiuation-2018
Subject:MICROBIOLOGY
QIJ'i?S'I"ION'?BANK
Time:2hoursMax.Nlarks??
l.ExperimentQuestions(12I?arks)
~1.Findnu:thequantityofProteinpresentinthegivensampleby3iuretll.o\vry's
wcdandplotastwitlardgraphof[rrtrlcinbycalorimetry(Theexaminer\vil|
provideunknownsampleandimcessaryreagents).
Z.FindvulLiceamountofDNApresentinlllugivenseuuplubyl?grheuylans-Inc
mclivutlandplntastandardgrapho.?i)'I\'/\bycalorimetry(lhct-"xatnincrwill
provideunknownsampleandnecessaryreagents).
3.Plotastandardgraphnl?RNAbycalorimetryusingOrcittolmethodandfindout
thequantityofRNApresentinthegiver.sample(Theexamine."willprovide
tuiknoxvttsampleandnecessaryreagents).
lsoiategenomicI).\JAfromthegivensample.Obseweandreporttheresult.
Asampleisprovidedtoyou.Demonstrateelectrophorelicseparationbymixing
Agarosegelelectrophoresisandinterpretyourresuil.
6.ProblemstaintedtoDNA,RNAcharaetcrisliux.TranscriptionandTmnslaliun
(Theexaminerscantareparcprobl?m?tobesolvedbyritestudents].
5".?-
ll.Spottings(4x2=8Marks)
7.Calorimeter
8.Cuvette
9.Micropipette
l?.(Yentrifuge
.__-~~~~~'"'11.PCRkit
?QLE?l2.Geleieutroplzoresisunit
lK-DPHrezigentwithlabel
l4.'l'l:Jbuffer
IS.DiagrammaticalrepresentationofLaoopercn
l6.Biuretreagentwithlabel
17.Orcinolreagentwithlabel
l8.GraphshowingtheestimationofDNAwithlabel
l9.GraphshowingtheestimationofRNAwithlabel-
20.Graphshowingtheestimationof?utcinwithlalzcl
III.Record&viva(SMarks)
This post was last modified on 21 November 2019