Download OU (Osmania University) B.Sc Fifth Year (5th Year) B.Sc Botany Practical Question Bank Question Paper
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Whendihybridplankwaeintapollinated,6%oftheprogenywaefoundtopossesovoid
shapedseedcapsule.Theotha94%oftheprogenyhadtriangularshapedseedcapsules.
a)Whattumfactorsepistaticratioisapproximatedbytheprogeny?
b)Whattypeofintaactionisopaative?
v)Incorn,adominantgeneCproducescoloredaleuroue;itsrecessiveallelecproducescolorless.
AnothadominantgeneSlrproduces?rllplumpkanels;ikrecessivealleleslrproducesshrunken
kanelsduetocollapsingoftheendospam.Athirddominantgene?V:producesnormalstarchy
endospamandikrecessiveallelew:produceswaxystarch.Ahomozygousplantfromaseedwith
colorless,plump,andwaxyendospamiscrossedtoahomozygousplant?omaseedwithcolored,
shrunken,andstarchyendospam.TheFlistestcrossedtoacolorless,shrunken,waxystrain.
Theprogenyseedexhibitthefollowingphenotypes:
113colorless,Srrunken,starchy:
4colored,Plump,starchy:
2708colorless,plump,Waxy:
626colorless,Plump,starchy:
2colorless,Shrunken,waxy:
116colored,P1ump,waxy:
2538colored,Shnmken,starchy:
601colored,Shrunken,waxy.
(a)constructageneticmapforthisregionofthechromosome.Roundallcalculationstothenearest
tenthofapacent.(b)Calculatetheintafaenceinthisregion.
4.Slides(2x2=4marks)
C)Cellorganelles
i)Mitochondria
ii)Choroplast
iii)GolgiComplex
D)Chromosomes
i)PolyteneChromosome
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Whendihybridplankwaeintapollinated,6%oftheprogenywaefoundtopossesovoid
shapedseedcapsule.Theotha94%oftheprogenyhadtriangularshapedseedcapsules.
a)Whattumfactorsepistaticratioisapproximatedbytheprogeny?
b)Whattypeofintaactionisopaative?
v)Incorn,adominantgeneCproducescoloredaleuroue;itsrecessiveallelecproducescolorless.
AnothadominantgeneSlrproduces?rllplumpkanels;ikrecessivealleleslrproducesshrunken
kanelsduetocollapsingoftheendospam.Athirddominantgene?V:producesnormalstarchy
endospamandikrecessiveallelew:produceswaxystarch.Ahomozygousplantfromaseedwith
colorless,plump,andwaxyendospamiscrossedtoahomozygousplant?omaseedwithcolored,
shrunken,andstarchyendospam.TheFlistestcrossedtoacolorless,shrunken,waxystrain.
Theprogenyseedexhibitthefollowingphenotypes:
113colorless,Srrunken,starchy:
4colored,Plump,starchy:
2708colorless,plump,Waxy:
626colorless,Plump,starchy:
2colorless,Shrunken,waxy:
116colored,P1ump,waxy:
2538colored,Shnmken,starchy:
601colored,Shrunken,waxy.
(a)constructageneticmapforthisregionofthechromosome.Roundallcalculationstothenearest
tenthofapacent.(b)Calculatetheintafaenceinthisregion.
4.Slides(2x2=4marks)
C)Cellorganelles
i)Mitochondria
ii)Choroplast
iii)GolgiComplex
D)Chromosomes
i)PolyteneChromosome
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Whendihybridplankwaeintapollinated,6%oftheprogenywaefoundtopossesovoid
shapedseedcapsule.Theotha94%oftheprogenyhadtriangularshapedseedcapsules.
a)Whattumfactorsepistaticratioisapproximatedbytheprogeny?
b)Whattypeofintaactionisopaative?
v)Incorn,adominantgeneCproducescoloredaleuroue;itsrecessiveallelecproducescolorless.
AnothadominantgeneSlrproduces?rllplumpkanels;ikrecessivealleleslrproducesshrunken
kanelsduetocollapsingoftheendospam.Athirddominantgene?V:producesnormalstarchy
endospamandikrecessiveallelew:produceswaxystarch.Ahomozygousplantfromaseedwith
colorless,plump,andwaxyendospamiscrossedtoahomozygousplant?omaseedwithcolored,
shrunken,andstarchyendospam.TheFlistestcrossedtoacolorless,shrunken,waxystrain.
Theprogenyseedexhibitthefollowingphenotypes:
113colorless,Srrunken,starchy:
4colored,Plump,starchy:
2708colorless,plump,Waxy:
626colorless,Plump,starchy:
2colorless,Shrunken,waxy:
116colored,P1ump,waxy:
2538colored,Shnmken,starchy:
601colored,Shrunken,waxy.
(a)constructageneticmapforthisregionofthechromosome.Roundallcalculationstothenearest
tenthofapacent.(b)Calculatetheintafaenceinthisregion.
4.Slides(2x2=4marks)
C)Cellorganelles
i)Mitochondria
ii)Choroplast
iii)GolgiComplex
D)Chromosomes
i)PolyteneChromosome
B.Sc.(CBCS)Botany-HIYear
Semester-V
Elective-I:Ecology&Biodiversity
PracticalSyllabus
1.StudyofplantcommunitiesbyQuadratMethod(8h)
2.Estimationofcarbonatesandbicarbonatesinthegivenwatasample.(4h)
3.Detaminationofsoiltexture(compositionofclay,sandsiltetc.)andpH.(2h)
4.Studyofmorphologicalandanatomicalcharactaisticsofplantcommunitiesusinglocally(8h)
availableplantspedes:Hydrophytes(Eichhomia,Hydrilla,Pistia,Nymphaea,
Vallisnaia),Xaophytes:(Asparagus,Opuntia,Euphorbiamelii),(Casuarina,Calotropis).
5.Valueofbiodivasity(8h)
a)Medidnalvalue:Catharanthus,TinosporaandEmblica
b)TimbaValue:Acada,TectonaandAzardirachta
c)AestheticValue:Mangifera,Ficus,Ocimun
d)Assesentoflocalbiodivasity.
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Whendihybridplankwaeintapollinated,6%oftheprogenywaefoundtopossesovoid
shapedseedcapsule.Theotha94%oftheprogenyhadtriangularshapedseedcapsules.
a)Whattumfactorsepistaticratioisapproximatedbytheprogeny?
b)Whattypeofintaactionisopaative?
v)Incorn,adominantgeneCproducescoloredaleuroue;itsrecessiveallelecproducescolorless.
AnothadominantgeneSlrproduces?rllplumpkanels;ikrecessivealleleslrproducesshrunken
kanelsduetocollapsingoftheendospam.Athirddominantgene?V:producesnormalstarchy
endospamandikrecessiveallelew:produceswaxystarch.Ahomozygousplantfromaseedwith
colorless,plump,andwaxyendospamiscrossedtoahomozygousplant?omaseedwithcolored,
shrunken,andstarchyendospam.TheFlistestcrossedtoacolorless,shrunken,waxystrain.
Theprogenyseedexhibitthefollowingphenotypes:
113colorless,Srrunken,starchy:
4colored,Plump,starchy:
2708colorless,plump,Waxy:
626colorless,Plump,starchy:
2colorless,Shrunken,waxy:
116colored,P1ump,waxy:
2538colored,Shnmken,starchy:
601colored,Shrunken,waxy.
(a)constructageneticmapforthisregionofthechromosome.Roundallcalculationstothenearest
tenthofapacent.(b)Calculatetheintafaenceinthisregion.
4.Slides(2x2=4marks)
C)Cellorganelles
i)Mitochondria
ii)Choroplast
iii)GolgiComplex
D)Chromosomes
i)PolyteneChromosome
B.Sc.(CBCS)Botany-HIYear
Semester-V
Elective-I:Ecology&Biodiversity
PracticalSyllabus
1.StudyofplantcommunitiesbyQuadratMethod(8h)
2.Estimationofcarbonatesandbicarbonatesinthegivenwatasample.(4h)
3.Detaminationofsoiltexture(compositionofclay,sandsiltetc.)andpH.(2h)
4.Studyofmorphologicalandanatomicalcharactaisticsofplantcommunitiesusinglocally(8h)
availableplantspedes:Hydrophytes(Eichhomia,Hydrilla,Pistia,Nymphaea,
Vallisnaia),Xaophytes:(Asparagus,Opuntia,Euphorbiamelii),(Casuarina,Calotropis).
5.Valueofbiodivasity(8h)
a)Medidnalvalue:Catharanthus,TinosporaandEmblica
b)TimbaValue:Acada,TectonaandAzardirachta
c)AestheticValue:Mangifera,Ficus,Ocimun
d)Assesentoflocalbiodivasity.
B.Sc(CBCS)Botany-IIIYear
Semester-V:Elective-I
Time:21/2hrsEcology&BiodiversityLlax.marks:25
PracticalQuestionBank
1.(1x6=6M)
i)Caliculate?equencyanddensityofthegivenQuadrates
2.(1x4=iM)
i)Estimationofcarbonatesinthegivenwatersample
ii)EstimationofBicarbonatesinthegivenwatasample
iii)Detaminationofsoiltexture(compositionofc.lay&silt)andPH.
3.(3><2=6M)
a)i)Hydrilla,ii)Calotropis,iii)Opuntia
b)i)PIstia,ii)Euphorbiamelii,iii)Casaurina
c)i)Eichornia,ii)Asparagusiii)Calotropis
d)i)Vallisnaia,ii)Opuntia,iii)Casurina
4.(2><2=4M)
a)i)Nymphaea,ii)Casurarina,iii)Pistia,iv)Asparagus
b)i)Eichornia,ii)Asparagus
5.(3x1=3M)
a)i)Emblica,ii)Tectona,iii)Ocimum
b)i)Catharanthus,ii)Acada,iii)Ficus
c)i)Tinospora,ii)Azadirachta,iii)Mangifaa
6.Record2Marks
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Whendihybridplankwaeintapollinated,6%oftheprogenywaefoundtopossesovoid
shapedseedcapsule.Theotha94%oftheprogenyhadtriangularshapedseedcapsules.
a)Whattumfactorsepistaticratioisapproximatedbytheprogeny?
b)Whattypeofintaactionisopaative?
v)Incorn,adominantgeneCproducescoloredaleuroue;itsrecessiveallelecproducescolorless.
AnothadominantgeneSlrproduces?rllplumpkanels;ikrecessivealleleslrproducesshrunken
kanelsduetocollapsingoftheendospam.Athirddominantgene?V:producesnormalstarchy
endospamandikrecessiveallelew:produceswaxystarch.Ahomozygousplantfromaseedwith
colorless,plump,andwaxyendospamiscrossedtoahomozygousplant?omaseedwithcolored,
shrunken,andstarchyendospam.TheFlistestcrossedtoacolorless,shrunken,waxystrain.
Theprogenyseedexhibitthefollowingphenotypes:
113colorless,Srrunken,starchy:
4colored,Plump,starchy:
2708colorless,plump,Waxy:
626colorless,Plump,starchy:
2colorless,Shrunken,waxy:
116colored,P1ump,waxy:
2538colored,Shnmken,starchy:
601colored,Shrunken,waxy.
(a)constructageneticmapforthisregionofthechromosome.Roundallcalculationstothenearest
tenthofapacent.(b)Calculatetheintafaenceinthisregion.
4.Slides(2x2=4marks)
C)Cellorganelles
i)Mitochondria
ii)Choroplast
iii)GolgiComplex
D)Chromosomes
i)PolyteneChromosome
B.Sc.(CBCS)Botany-HIYear
Semester-V
Elective-I:Ecology&Biodiversity
PracticalSyllabus
1.StudyofplantcommunitiesbyQuadratMethod(8h)
2.Estimationofcarbonatesandbicarbonatesinthegivenwatasample.(4h)
3.Detaminationofsoiltexture(compositionofclay,sandsiltetc.)andpH.(2h)
4.Studyofmorphologicalandanatomicalcharactaisticsofplantcommunitiesusinglocally(8h)
availableplantspedes:Hydrophytes(Eichhomia,Hydrilla,Pistia,Nymphaea,
Vallisnaia),Xaophytes:(Asparagus,Opuntia,Euphorbiamelii),(Casuarina,Calotropis).
5.Valueofbiodivasity(8h)
a)Medidnalvalue:Catharanthus,TinosporaandEmblica
b)TimbaValue:Acada,TectonaandAzardirachta
c)AestheticValue:Mangifera,Ficus,Ocimun
d)Assesentoflocalbiodivasity.
B.Sc(CBCS)Botany-IIIYear
Semester-V:Elective-I
Time:21/2hrsEcology&BiodiversityLlax.marks:25
PracticalQuestionBank
1.(1x6=6M)
i)Caliculate?equencyanddensityofthegivenQuadrates
2.(1x4=iM)
i)Estimationofcarbonatesinthegivenwatersample
ii)EstimationofBicarbonatesinthegivenwatasample
iii)Detaminationofsoiltexture(compositionofc.lay&silt)andPH.
3.(3><2=6M)
a)i)Hydrilla,ii)Calotropis,iii)Opuntia
b)i)PIstia,ii)Euphorbiamelii,iii)Casaurina
c)i)Eichornia,ii)Asparagusiii)Calotropis
d)i)Vallisnaia,ii)Opuntia,iii)Casurina
4.(2><2=4M)
a)i)Nymphaea,ii)Casurarina,iii)Pistia,iv)Asparagus
b)i)Eichornia,ii)Asparagus
5.(3x1=3M)
a)i)Emblica,ii)Tectona,iii)Ocimum
b)i)Catharanthus,ii)Acada,iii)Ficus
c)i)Tinospora,ii)Azadirachta,iii)Mangifaa
6.Record2Marks
B.Sc.(CBCS)BOTANY:IIIYEAR
Semester-V
Elective_II:Horticulture
PracticalSyllabus
1.Gardentoolsandimplanents.(2h)
2.Identi?cationandeconomicvaluesofanytwooftropicalandsubtropical
vegetable,fruit,?owaandornamentalcrops.(2h)
3.Propagationpracticesbyseed,Vegetativepropagation(Rhizome,bulb,corm),cutting,
layering,budding,gaftingwithtwoexamples.(6h)
4.Seedpropagation-seedtreatments,sowingandseedlingproduction.(4h)
5.Nursaypractices,transplanting,?eldpreparation,sowingfplanting,useofhabicides,
topdressingoffatilizasanduseofgowthregulators.(4h)
6.Nursaycontainas,media,pottingandrepottingofplants,hardeningofplankinnursery,
shaderegulationinnursay,plantprotectioninnursayplants(Danonstration)(4h)
7.Packingnursayplankforlocalandlongdistancemarkets.(Danonstration)(2h)
8.Makingoforganic-compost.(6h)
B.Sc.Botany-HIYear
Semester-V:Paper-V
CellBiologyandGenetics
PracticalSyllabus
1.Demonstrationofcytochemicalmethods:Fixationofplantmaterialandnuclearstaining
formitoticandmeioticstudies.(4h)
2.StudyofvariousstagesofmitosisusingcytologicalpreparationofOnionroottips.(4h)
3.StudyofvariousstagesofmeiosisusingcytologicalpreparationofOnion?owerbuds.(2h)
4.Solvinggeneticproblemsrelatedtomonohyhrid,dihyhridratioincompletedominanceand
interactionofgenes(minimumofsixproblemsineachtopic).(8h)
S.Constructionoflinkagemaps;tumandthreepointtestcross.(4h)
6.Studyofultrastructureofcellorganellesusingphotographers.
Chloroplast,Mitochondria,Nucleus,Ribosomes,Endoplasmicreticulum,andGolgicomplex.(4h)
7.StudyofSpedaltypesofChromosomes(PolytenechromosomeandLamphrushchromosomes-
Pcrmanentslide)(4h)
B.Sc.Botany-HIYear
Semester-V
Paper-V:CellBiologyandGenetics
PracticalQuestionBank
1
(A).(sMarks)
i).Prepareacytologicalslideofthegivenmataial(A),Identify8cdescribeanytummitoticstages
withwelllabeleddiagams.
2
(B)(6Marks)
i).Drosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody(g).A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_4l5
B)Vestigialwingsblackbody_405
C)Vestigialwingsgaybody_92
D)Normalwingsblackbody_88
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatisthearranganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
ii).Dosophilamelanogasmrlongwings(vg+)isdominantovavestigialwings(vg)andgay
body(g+)isdominantovablackbody.A?illyFlhetaozygouslongwingswithgaybody
crossedwithvestigialwingsandblackbody.Theprogenyobtainedasfollows.
A)Longwingsgaybody_92
B)vestigialwingsblackbody_88
C)vestigialwingsgaybody_415
D)Normalwingsblackbody_405
i)Thesetwogenesaresegegatingindependentlyornot
ii)Constructageneticmapinthisregionofchromosome
iii)Whatistheananganentofgenes,cisortrans
iv)Calculatethegeneticdistanceinbetweenthesetwogenes.
iii)
A
gem?a||?d?fQrk@d?(t)producesshortened,bent,orsplitbristlesandhairs
inDrosophila.Anothergenecalled?outstpetghed"(od)resultsinwingsbeing
carriedatrightanglestothebody.
A
thirdgenecalled?game?(S)Produces
pinkisheyecolourinyoung?ies.Wild-typefemalesheterozygousatallthreeloci
werecrossedtowild-typemales.TheF,dataappearbelow.
Marks
:
(1
1)
F1:Females:allwildtype
Males:57garnet,outstretched
419
garnet,forked
60forked
1outstretched,forked
2
garnet
439
outstretched
13wildtype
9outstretched,garnet,forked
1000
(a)Whichgeneisinthemiddle?(b)Whatwaslinkagerelationshipbetweenalleleat
theforkedandoutstretchedlociinthematernalparent?(c)Whatwasthelinkage
relationshipbetweenallelesattheforkedandgarnetlociinthematemalparent?(d)
Onwhatchromosomedothesethreegenesreside?(e)Calculatethemapdistances.(f)
constructageneticmapforthisregionofthechromosome(g)Howmuch
interferenceisoperative?
iv).InasnapdragonRedcolourof?owa(R)isincompletelydominantovawhite(r)of?owa.
Theheterozygousconditionbeingpink(Rr).Tallness(T)iscompletelydominantovadwar?ress
(t).Ahetaozygoustallred?owaplantiscrossedwi?rdwarfandwhite?owaedplant.Find
outphenotype8cgenotypeofprogenyofF18:F;genaations.
v).Whenatallplantisselfed,itproduced64plantshavingtall8cdwarfphenotypeshowmany
aretallandhowmanydwarf?
vi).ln4'0clockplantflower.redcolourflowers(RR)isincompletelydominantover\vhite(rr).
51?h?emlygm?Plant55118Pink?l-?VCIS-whenacrossismadebetweenatedflowered4'0
clockplantwi?rawhite?owaedone,FindoutthephenotypicprogenyinFlandF2
genaations?
vii).Whatwillbe?reresultofsel?ngFlgenaationinacrosswhenroundandyellowseeded
peaplants(YYRR)arecrossedwi?rgeenandwrinkled(yyrr)seededpeaplants?
viii).Ingardenpeas,tallplanthabit(T)isdominantovadwarf(t),geenpeas(G)ovayellow(g),
bringoutacrossbetweentallyellowanddwarfgeenandobtainFl8cF2,give?repercentageof
tallgeenhomozygousamongF2.GivetheF2genotypicratio?
ix).Inasnapdragon,redflowa(RR)isincompletelydominantovawhite(rr).Thehetaozygous
conditionbeing(Rr).Thenormalbroadleaves(BB)isincompletelydominantovanarrowleaves
(bb).Thehetaozygousconditionbeing?reintamediate(Bb).Whenred?owaedbroadleaves
plantiscrossedwithwhite?owaednarrowleafplant,?nd?rephenotypeofprogenyofFlandF2
genaation?
x).Inacrossbetweenred?owaedplantandwhite?owaedplantyieldsplantsofboththecolours
inequalproportion,butacrossbetweentwowhite?owaedplantsyieldsonlywhite?owaed
plants.Whatcouldbethegenotypesoftheparentsandwhichphenotypeisrecessive?
xi).Inapeatallplantwithroundseedsiscrossedwithadwarfplanthavingwrinkledseeds.The
progenyobtainedisintheratioofonetallplantwithroundseeds,onetallplantwithwrinkled
seeds,onedwarfplantwithroundseeds,andonedwarfplantwithwrinkledseeds.Findoutthe
genotypesoftwoparentsinpeaplants.RoundisdominantovawrinkledTallisdominanton
dwarf.
3(C).5Marks
i).Matingbetweenblackratsofidenticalgenotypesproducedo?pringsasfollows
l4-creamcoloured
47-b1ack
19-albino
a)Whatarethegenotypesoftheparents8ctheo?spring?(Useyourownsymbols)
b)Whatepistaticratioisapproximatedbytheseo?springs?
c)Whattypeofepistasisisopaative?
ii).Coatcolourofdog'sdependupontheactionofatleast2genes.Atonelocusadominant
epistanicinhibitorofcoatcolourpigment(I-)preventstheexpressionofcolourallelesatanotha
independen?yassortinglocusproducingwhitecoatcolour.Whentherecessiveconditionexistsat
theinhibitorlocus(ii),theallelesofthehypostaticlocusmaybeexpressed,iiB-producingblack8c
iibbproducingbrown.Whendihybridwhitedogsarematedtogetha,detamine,
a)Thephenotypicproportionsexpectedintheprogeny
iii).Tumwhite?owaedstrainofthesweatpea(Lathyrusodoratus)waecrossed,producinganF1
withonlypurple?owas.RandomcrossingamongtheFlproduced96progenyplants,53
exhibitingpurple?owas8c43withwhite?owers.
a)WhatphenotypicratioisapproximatedbytheF2?
b)Whattypeofintaactionisinvolved?
c)Whatwaetheprobablegenotypesoftheparentalstrains?
iv).Aplantofthegenuscapcellacommonlycalledasshephadspurseproducesaseedcapsule.The
shapeofwhichiscontrolledbytumindependen?yassortinggenes,representedbysymbolsAand
B.Whendihybridplankwaeintapollinated,6%oftheprogenywaefoundtopossesovoid
shapedseedcapsule.Theotha94%oftheprogenyhadtriangularshapedseedcapsules.
a)Whattumfactorsepistaticratioisapproximatedbytheprogeny?
b)Whattypeofintaactionisopaative?
v)Incorn,adominantgeneCproducescoloredaleuroue;itsrecessiveallelecproducescolorless.
AnothadominantgeneSlrproduces?rllplumpkanels;ikrecessivealleleslrproducesshrunken
kanelsduetocollapsingoftheendospam.Athirddominantgene?V:producesnormalstarchy
endospamandikrecessiveallelew:produceswaxystarch.Ahomozygousplantfromaseedwith
colorless,plump,andwaxyendospamiscrossedtoahomozygousplant?omaseedwithcolored,
shrunken,andstarchyendospam.TheFlistestcrossedtoacolorless,shrunken,waxystrain.
Theprogenyseedexhibitthefollowingphenotypes:
113colorless,Srrunken,starchy:
4colored,Plump,starchy:
2708colorless,plump,Waxy:
626colorless,Plump,starchy:
2colorless,Shrunken,waxy:
116colored,P1ump,waxy:
2538colored,Shnmken,starchy:
601colored,Shrunken,waxy.
(a)constructageneticmapforthisregionofthechromosome.Roundallcalculationstothenearest
tenthofapacent.(b)Calculatetheintafaenceinthisregion.
4.Slides(2x2=4marks)
C)Cellorganelles
i)Mitochondria
ii)Choroplast
iii)GolgiComplex
D)Chromosomes
i)PolyteneChromosome
B.Sc.(CBCS)Botany-HIYear
Semester-V
Elective-I:Ecology&Biodiversity
PracticalSyllabus
1.StudyofplantcommunitiesbyQuadratMethod(8h)
2.Estimationofcarbonatesandbicarbonatesinthegivenwatasample.(4h)
3.Detaminationofsoiltexture(compositionofclay,sandsiltetc.)andpH.(2h)
4.Studyofmorphologicalandanatomicalcharactaisticsofplantcommunitiesusinglocally(8h)
availableplantspedes:Hydrophytes(Eichhomia,Hydrilla,Pistia,Nymphaea,
Vallisnaia),Xaophytes:(Asparagus,Opuntia,Euphorbiamelii),(Casuarina,Calotropis).
5.Valueofbiodivasity(8h)
a)Medidnalvalue:Catharanthus,TinosporaandEmblica
b)TimbaValue:Acada,TectonaandAzardirachta
c)AestheticValue:Mangifera,Ficus,Ocimun
d)Assesentoflocalbiodivasity.
B.Sc(CBCS)Botany-IIIYear
Semester-V:Elective-I
Time:21/2hrsEcology&BiodiversityLlax.marks:25
PracticalQuestionBank
1.(1x6=6M)
i)Caliculate?equencyanddensityofthegivenQuadrates
2.(1x4=iM)
i)Estimationofcarbonatesinthegivenwatersample
ii)EstimationofBicarbonatesinthegivenwatasample
iii)Detaminationofsoiltexture(compositionofc.lay&silt)andPH.
3.(3><2=6M)
a)i)Hydrilla,ii)Calotropis,iii)Opuntia
b)i)PIstia,ii)Euphorbiamelii,iii)Casaurina
c)i)Eichornia,ii)Asparagusiii)Calotropis
d)i)Vallisnaia,ii)Opuntia,iii)Casurina
4.(2><2=4M)
a)i)Nymphaea,ii)Casurarina,iii)Pistia,iv)Asparagus
b)i)Eichornia,ii)Asparagus
5.(3x1=3M)
a)i)Emblica,ii)Tectona,iii)Ocimum
b)i)Catharanthus,ii)Acada,iii)Ficus
c)i)Tinospora,ii)Azadirachta,iii)Mangifaa
6.Record2Marks
B.Sc.(CBCS)BOTANY:IIIYEAR
Semester-V
Elective_II:Horticulture
PracticalSyllabus
1.Gardentoolsandimplanents.(2h)
2.Identi?cationandeconomicvaluesofanytwooftropicalandsubtropical
vegetable,fruit,?owaandornamentalcrops.(2h)
3.Propagationpracticesbyseed,Vegetativepropagation(Rhizome,bulb,corm),cutting,
layering,budding,gaftingwithtwoexamples.(6h)
4.Seedpropagation-seedtreatments,sowingandseedlingproduction.(4h)
5.Nursaypractices,transplanting,?eldpreparation,sowingfplanting,useofhabicides,
topdressingoffatilizasanduseofgowthregulators.(4h)
6.Nursaycontainas,media,pottingandrepottingofplants,hardeningofplankinnursery,
shaderegulationinnursay,plantprotectioninnursayplants(Danonstration)(4h)
7.Packingnursayplankforlocalandlongdistancemarkets.(Danonstration)(2h)
8.Makingoforganic-compost.(6h)
B.Sc(CBCS)BOTANY:IIIYEAR
Semester-V
Elective-II:Horticulture
Time:21/2lrrsPracticalQuestionBankLlax.marks:25
1.MajorExpaiment-(A)(lx8Marks)
i)AirLayaing
ii)Grafting
2.MinorExpaiment-(B)(lx6Marks)
i)Identi?cation,NutritiveandEconomicvalueofvegetableorfruit
Vegetables:a)Cabbageb)Beansc)Capsicum
d)EggPlante)Okraf)Potato
Fruit:a)PineAppleb)Papayac)JackFruit
d)Coconute)Annonaf)Watamelon
ii)Makingoforganiccompost-Flowchart
3.Spottas:(C,D,E)(3><3=9Marks)
(C)Vegetativepropagativeorgan:
i)Rhizomeii)Runnasiii)Tubas
iv)Cormsv)Suckas
(D)HorticultureGardentoos:
i)Raketoolii)Troweliii)Shovel
iv)Lawnmowav)Edgavi)Primingknives
(E)Growthhormones:
i)Bonsaispecimenii)Auxins
iii)Cytokyninsiv)Gibbaellins
This post was last modified on 21 November 2019