Download PTU (Punjab Technical University) B.Tech (Bachelor of Technology) / BE (Bachelor of Engineering) 2021 January BT-Biotechnology 7th Sem 71843 Bioprocess Technology Previous Question Paper
Total No. of Pages : 02
Total No. of Questions : 18
B.Tech. (BT) (2012 to 2017) (Sem.?7)
BIOPROCESS TECHNOLOGY
Subject Code : BTBT-701
M.Code : 71843
Time : 3 Hrs. Max. Marks : 60
INST RUCT IONS T O CANDIDAT ES :
1.
SECT ION-A is COMPULSORY c onsisting of TEN question s carrying TWO marks
each .
2 .
SECT ION-B c ontains F IVE questions c arrying FIVE marks eac h and s tud ents
have to atte mpt ANY FOUR questio ns.
3 .
SECT ION-C contains THREE questions carrying T EN marks e ach and s tudents
have to atte mpt ANY TWO questions .
SECTION-A
Discuss briefly :
1.
Write down two basic differences between bioprocessing and chemical processing.
2.
What is mean by bioconversion process?
3.
Highlight at least one advantage and one disadvantage of batch over continuous
fermentation process.
4.
Explain continuous cell line.
5.
When filter sterilization is recommended?
6.
Define Respiratory Quotient.
7.
In recent days, what problems associated with the bakers yeast growth in molasses
encourage the choice of starchy materials from cereal grains as growth medium?
8.
What is the major difference between the primary metabolite and the secondary metabolite
production in relation to the microbial growth phase?
9.
Explain feedback inhibition.
10. What are major ways of strain development?
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SECTION-B
11. Thermal death of microorganisms in the liquid medium follows first order kinetics. If the
initial cell concentration in the fermentation medium is 108 cells / ml and the final
acceptable contamination level is 10-3 cells, for how long should 1.0 m3 medium be treated
at temperature of 120 ?C to achieve acceptable load? Consider the thermal deactivation rate
constant at 120 ?C is 0.23 / min.
12. What are the basic features of a fermenter? Draw a schematic to indicate them.
13. Comment on `diffusion limitation in immobilized enzyme system'.
14. Briefly explain the `Chelating Agent' and `Growth Factor' in media design.
15. Discuss chromatography technique in downstream processing.
SECTION-C
16. Describe ethanol fermentation.
17. A strain of mold was grown in batch culture on glucose and the following data were
obtained :
Time (h)
Cell concentration
Glucose
(g/1)
concentration (g/1)
0
1.25
100
9
2.45
97
16
5.1
90.4
23
10.5
76.9
30
22
48.1
34
33
20.6
36
37.5
9.38
40
41
0.63
a. Calculate the maximum net specific growth rate.
b. Calculate the apparent growth yield.
c. What maximum cell concentration could one expect if 150 g of glucose were used with
the same size inoculum?
18. Discuss the feedback inhibition problem relating the L-Lysine production. Describe
L-Lysine production including the production process, different media sources and
influencing factors and downstream processing.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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This post was last modified on 26 June 2021