Download AKTU B-Tech 5th Sem 2015-2016 Genetic Engineering Nbt 501Ebt 501 Question Paper

Download AKTU (Dr. A.P.J. Abdul Kalam Technical University (AKTU), formerly Uttar Pradesh Technical University (UPTU) B-Tech 5th Semester (Fifth Semester) 2015-2016 Genetic Engineering Nbt 501Ebt 501 Question Paper

Printed Pages: 4 382/360 NBT?SOI/EBT?SOI
(Following Paper ID and Roll No; to be ?lled in your
Answer Book)
Roll No.
B.Tech
(SEM. V) THEORY EXAMINATION, 2015?16
GENETIC ENGINEERING
[Time:3 hours] [Total Marks: 100]
SECTION-A
Note :Attempt all parts. All parts carry equal marks. Write
answer of each part in short. (2 X 10:20)
1. (a) What is the difference between northern blot and
southern blot; ?
(b) Differentiate bgtween isoschizomers and
neoschizomers with examples.
(c) What is star activity? ls star activity useful is gene
cloning? '
(d) Differentiate between E.coli encoded and phage
T4 encoded DNA ligase.
1600 ? (1) P.T.O.

(e) What are restriction endonucleases? Which class
of restrictionendonuclease can be used in
recombinant DNA technology?
(0 List various physical DNA delivery methods in a
cell._
(g) Describe the mechanism of signalling by nitric
oxide (NO).
(h) Differentiate between cDNA library and genomic
library.
(i) What are the ion-channel linked receptors?
0) How do different cells respond differently to same
. extracellular Signal molecule?
SEC'I?IONQB
Attempt any five questions from this section. (10X5=50)
2.
1600
Describe the features that distinguiSh cosmici, phasmid,
phagemid, BAC and YAC cloning vectors.
Describe the various steps of polymerase chain
reaction.What is the advantage of using RT-PCR?
(2) NBT-501/EBT-50l
What are different types of receptors present in cells.
What is the difference between G-protein-linked
receptors and Enzyme-linked receptors?
Describe paracrine, endocrine and synaptic signalling.
What are the different classes of cell-surface receptor
protein? Describe in detail about any two of them.
What is site speci?c recombination? Describe the
different steps involved in site speci?c recombination.
Describe various DNA delivery methods.
How foreign genes are introduced into a plant using Ti
plasmid of A grobacterium?
SECTION?C
Attempt any two questions from this section. (15 x2:30)
What are restriction endonucleases? Describe four
types of restriction and modi?cation system. Which
one of it can be used in recombinant DNA technology?
(3) P.T.O.

11. Describe chemical induction and electroporation.
12. What are transposons? Differentiate between
transposable elements in prokaryotes and eukaryotes.
1 600 (4) NBT-SOI/EBT-SOI