Download AKTU B-Tech 5th Sem 2015-2016 Modern Analytical Techniques Nbt 505 Question Paper

Download AKTU (Dr. A.P.J. Abdul Kalam Technical University (AKTU), formerly Uttar Pradesh Technical University (UPTU) B-Tech 5th Semester (Fifth Semester) 2015-2016 Modern Analytical Techniques Nbt 505 Question Paper

Printed Pages: 3 ' 226 NBT?505
(Following Paper ID and Roll N o. to be filled in your
Answer Book)
Roll No.
B.TECH.
(SEM. V) THEORY EXAMINATION,~ 2015-16
MODERN ANALYTICALTECI-INIQUES
[Time:3 hours] - [MaximumMarksz 100'
.(
Nate : The Questidn Paper contain three S?Ctions.
Section-A
Q.~1 Attempt all parts. All parts carry equal marks. Write
answer of each (part in short. (2x10=20)
(a) What do ybu understand by bathochromic and
hydrochromic shi? ?
(b) Discuss principle of chromatography.
(c) What do you mean by Mclafferty re-arrangement?
(d) How number and position of bands is calculated in
IR spectrum of a compound ?
1100 7 . '(1) P.T.O.

(e) De?ne isoelectric focusing.
' (f) What do you understand by membrane fouling ?2
(g) Write the role of ammonium persulfate and
dithiothreitol in SDS-PAGE.
(h) Give two applications of ion-exchange
chromatography.
(i) De?ne molar absorption coefficent.
0) Write the principle of electrophoresis.
? Section-B ?
N ote: Attempt any ?ve questions ?'om this section : I
(10x5=50)
Q2? De?ne Beer-Lambert?s law. How it is useful? in
determining the concentration of an analyte ?
Q3. What are the different components of HPLC ? How
mobile phase is optimised ?
Q4. Write the details of atomic absorption spectrography. ?7
05. What IS spin-Spin coupling ? Explain giving examples
and applications.
1100 ' (2)
? NBT-SOS , i
3
,.._ 1...?... ... -,...~.~?-...~,~_?? 11.6..??Ww 1 A .
Q6. Explain sheilding and desheilding effect in NMR.
Q7. Explain the principle and applications of gel ?ltration
chromatography. How this technique ?is used for
determination of molecular weights of proteins ?
Q8. Discuss the methodology involved in separation of
proteins by polyacrylamide gel electrophoresis;
Q9. Discuss principle, instrumentation and applications of
Circular Dichroism.
Section-C ?
Note: Attempt any two questions from this section :
. (15x2=30)
Q10. Discuss theory, instrumentation and application of
centrifugation.
Q11. Explain various types of chromatography ? How they
differ from each other ?
Q12. Write a note on X-Ray deffraction technique. How this
is used in structural analysis of biomolecules.
_X?
1100 i (3)