Pharma D 2nd Year Pharmaceutical Microbiology Important Questions

Pharma D Second Year (Second Year) Pharmaceutical Microbiology Important Question Bank


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Essay Questions Pharma D 2nd Year



  1. Define Disinfection. Classify Disinfectants. Write in detail about evaluation of disinfectants.
  2. Write the different types of Media. Differentiate bacteria and fungi.
    Write the different methods of identification of bacteria.
  3. Discuss the factors affecting bacterial growth.
    What are the various types of nutrient media?
  4. Define immunity and elaborate on different types of immunity.
    What are antigen antibody interactions and its importance?
  5. Define Sterilization.
    Discuss the different methods of sterilization with examples. Add a note on validation.
  6. Explain the structure, nutritional requirements and
    reproduction of Bacteria.
  7. a) Outline the factors affecting the disinfectant action.
    b) Write in detail about Western blot techniques?
  8. a) Elaborate on the microbial assay of riboflavin and cyanocobalamine.
    b) Write in brief about type of culture media
  9. a). Define Immunity. Explain the classifications of immunity with examples.
    b) Microbiological assay of antibiotics.
  10. a) Describe the sterility testing for different Pharmaceutical
    preparations.  b) Counting of Bacteria
  11. a) Explain different type of ELISA and its importance.
    b) How will you separate macromolecules (DNA, RNA) by Agarose gel
    electrophoresis method?
  12. Write detail about symptom and treatment of following diseases:
    i) Malaria
    ii) Tuberculosis
    iii) Cholera
    iv) Hepatitis.
  13. Write on Bacteriostatic and bactericidal evaluation of disinfectant.
  14. Elaborate study about growth and reproduction of bacteria.
  15.  a) Classify Microbes with examples.
    Explain in detail about the morphology of Bacteria. (10)
    b) Give the different methods of isolation and identification of bacteria
    with special emphasis to Gram’s Staining technique. (10)
  16. Study of infectious diseases
    a) Meningitis.
    b) Syphilis.
    c) Gonorrhea and HIV.
  17. Explain the use of hot air oven and autoclave to control microorganisms.
  18. Classify immunity and describe active immunity with example
  19. Explain IMViC test in detail
  20. Elaborate on the structure of bacterial cell wall with a note on peptidoglycan synthesis.
  21. Explain about the Isolation and Reproduction of Bacteria.
  22. Define Sterilization and describe about the sterilization by heat and chemicals.
  23. Classify Disinfectants and discuss in detail about the Evaluation of Disinfectants.
  24. Microbiological assay of Antibiotics.
  25. Define validation and explain the validation process.
  26. Write in detail about asexual reproduction of bacteria and explain growth and
    cultivation of bacteria.
  27. Explain the various type of counting method.
  28. Discuss about factors affecting disinfecting agent.
  29. Explain in detail about the Bacterial Cell Morphology with neat diagram.
  30. Define and classify the different methods of Sterilization. Discuss in detail about
    Sterilization by Radiation method.
  31. Define and classify Immunity. Discuss the general principles and cells involved in
    Innate Immunity.
  32. Elaborate on: a) HIV b) Tuberculosis.
  33. Write in detail about western blot techniques
  34. Explain the structure and formation of Immunoglobulin. Discuss the significance
    of different classes of Immunoglobulin
  35. Write elaborately Synchronous culture method.
  36. Discuss in detail about gaseous sterilization method including their merits and
  37. Discuss in detail isolation methods and identification test for Bacteria.
  38. Write in detail on the principle, instrumentation, procedure, merits and demerits of
    moist heat and Dry Heat Sterilisation.
  39. Explain elaborately on antigen-antibody reactions
  40. Discuss in detail on:
    a) Typhoid b) Malarial infection.
  41. Define Bacteria. Explain the nutritional requirements and cultivation of bacteria.
  42. Define Sterilization. Describe the different methods of sterilization by heat.
  43. Define Immunity. Explain in details about types of immunity
  44. Define Microbiological assay and explain the microbiological assay of antibiotics.
  45. Discuss in detail about the morphological structure of Bacteria with neat diagram.
  46. Discuss the various methods of evaluation of disinfectants.
  47. Describe the various steps involved in Phagocytosis process
  48. Discuss on : a) Tuberculosis b) Hepatitis-B.
  49. a) Bacterial Growth Curve. b) Viable counting of bacteria
  50. Define Sterilization. Describe the sterilization by radiation and chemical agents.
  51. Microbiological assay of antibiotics.
  52. Malaria
  53. Explain different types of medium and methods to isolate the Bacteria.
  54. Describe gaseous sterilization including their merits and demerits
  55. Define Bacterial Exotoxins. Differentiate between exotoxin and endotoxin with
  56. Define Microbiological assay. Write the principle and procedure of
    Microbiological assay of Anti-biotics.
  57. Explain stepwise Western blot test procedure.
  58. Define Immunology. Classify various types of Immunity with examples.
  59. Describe features influence the growth of Bacteria.
  60. Discuss the morphological feature of Bacteria with diagram.



Short Questions



  1. Sterility Testing.
  2. Types of Immunity.
  3. Dry Heat Sterilization
  4. Antigen – Antibody Reaction
  5. Nutritional Requirement of Bacteria.
  6. Name different Blot techniques and explain Western Blot.
  7. Modes of transmission, diagnosis and treatment of any two viral diseases
  8. Classify different categories of disinfectants with examples and their uses
  9. Microbiological assays
  10. Principle involved in sterility testing
  11. Morphology and identification of fungi
  12. Differential staining.
  13. Acquired Immunity
  14. Cultivation of virus.
  15.  Evaluation of Disinfectants
  16. Antigen-Antibody reactions
  17. ELISA test.
  18. Microbiological assay of Vitamins.
  19. Tuberculosis
  20. Hepatitis
  21. Standardization of vaccines
  22. Immunization programme.
  23. Discuss the technique involved in Isolation of pure culture
  24. Write on widal test and Mantaux test.
  25. Classify the various process under moist heat sterilization
  26. Reproduction of bacteria
  27. What you know about HEPA filter
  28. Write the principle and procedure of gram staining technique
  29. Differentiate between exotoxin and endotoxin
  30. Outline the immunization chart
  31. Write on aerobic and anaerobic fungi
  32. Discuss about carbon source and energy source.
  33. Reproduction of Fungi.
  34. Isolation of Bacteria.
  35. Evaluation of preservatives in Pharmaceutical preparations.
  36. Structure and Types of Antigen
  37. PCR
  38. Mantoux Test.
  39. Microbiological assay of Vitamin B12.
  40. Malaria
  41. Hepatitis
  42. Multiplication of Viruses
  43. Preparation of Alum precipitated toxoids
  44. Differentiate active and passive immunity
  45. Write on Viable counting methods
  46. Write differential media with examples
  47. Define validation and explain its importance
  48. Give brief account on Toxin neutralization test
  49. Specify the Function of T cells and B cells
  50. Write on gaseous sterilization method
  51. Antigen antibody interactions
  52. How will you carryout test for Sterility?
  53. Outline about phagocytosis
  54. Expand HAT media and its function.
  55. Explain Selective media with example
  56. How will you Preserve the culture by soil and nitrogen gas method.
  57. Give a brief account of Polymer chain reaction
  58. Differentiate vaccine and sera
  59. Brief notes on total counting method
  60. Outline about gradient plate techniques
  61. Define immunity and explain naturally acquired active and passive immunity with
  62. Brief notes on widal test.
  63. Procedure and report of Shick’s Test
  64. Define and differentiate Exotoxin and Endotoxin
  65. Enriched media.
  66. Maintenance of cultures in laboratory
  67. Rickettsiae with examples
  68. Validation of Autoclave
  69. Explain Pasteurization.
  70. Method of counting bacteria by membrane filtration technique.
  71. Redial Walker Coefficient Test
  72. Importance of booster dose
  73. Discuss the life cycle of malarial parasite. Write a note on laboratory diagnosis
  74. Describe bacterial conjugation
  75. Define dye and explain different types of dyes with suitable example
  76. Define medium. What is the role of peptone, NaCl and agar in the medium?
  77. Classify fungi, with a note on economic importance of fungi.
  78. Describe the various Antigen- Antibody reactions in detail.
  79. Maintenance of Lab cultures.
  80. Cultivation of Virus
  81. Types of Immunity.
  82. Immunization programme.
  83. Western Blot test.
  84. Syphilis and Gonorrhea.
  85. What is stain? Explain Acid fast staining.
  86. Comment on carbon, nitrogen and energy requirement of bacteria.
  87. Explain isolation of bacteria by pour plate technique and spread plate technique.
  88. What is filtration? Explain any two filters used for sterilization
  89. Write a note on differences between Gram positive and Gram negative cell wall.
  90. Explain the process of B – cell maturation, activation and differentiation.
  91. Blotting technique
  92. Explain selective and differential medium for bacteria
  93. Different methods of bacterial counting
  94. Microbiological assay of Penicillin
  95. Reproduction methods of Yeast
  96. Evaluation of Bactericidal activity of disinfectants.
  97. Give the note on QBC test.
  98. Discuss about antigen antibody reaction.
  99. Cholera and its prevention
  100. Give an account of phagocytosis
  101. Write briefly on preservation of lab culture
  102. Microbiological assay of Riboflavin
  103. Schick’s test.
  104. Discuss morphological features of mold
  105. Give the notes on standardization of vaccine
  106. Discuss the various methods for evaluation of disinfectants
  107. Acid – Fast staining technique
  108. Microbiological assay of Vitamin by Turbidometric method
  109. Maintenance of lab cultures
  110. Explain the factors affecting disinfection action
  111. Southern blot test.
  112. Immunization programme.
  113. Malaria
  114. Typhoid
  115. Write briefly about evaluation of bactericidal activity of disinfectants.
  116. Discuss on moist heat sterilization
  117. Discuss about southern blotting techniques.
  118. Diagnosis and prevention of Cholera
  119. Write note on various methods of Viable counting of bacteria.
  120. Define staining of bacteria. Explain Gram’s staining technique.
  121. Cultivation of Viruses.
  122. Evaluation of Disinfectants.
  123. Acquired immunity
  124. Enzyme linked immunosorbent assay
  125. Tuberculosis
  126. Meningitis & HIV.
  127. Write about Widal Test
  128. Discuss briefly the morphology of Spirochetes.
  129. Explain total count techniques of Bacteria
  130. Write about meningitis
  131. Classify the disinfectant with examples
  132. Add a note on Microbiological assay of penicillin
  133. Differentiate selective and differential media with example
  134. Write about the maintenance techniques of lab cultures.
  135. Rickettsiae
  136. Validation of biological sterilization methods.
  137. Evaluation of preservatives in pharmaceutical formulations
  138. Bacterial exotoxins



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Pharma D Last 10 Years 2011-2021 Papers





Above question papers are applicable for below universities

Pondicherry University
NTR University of Health Sciences, VijayWada
Gandhi Institute of Technology and Management (GITAM University) Deemed, Visakhapatnam
Sri Venkateswara Institute of Medical Sciences (SVIMS), Tirupati
Srimanta Shankardeva University of Health Sciences
Statutory Autonomous, AIIMS
Aryabhatta Knowledge University, Patna
Indira Gandhi Instt of Medical Sciences (Deemed University), Patna
B.N. Mandal University
Veer Kunwar Singh University,Bihar
Ayush and Health Sciences University, Raipur
Punjab University
Chhattisgarh Ayush and Health Sciences University, Raipur
Guru Gobind Singh Indraprastha University
University of Jamia Hamdard (Hamdard University)
Delhi University
Goa University
Gujarat University
Saurashtra University
Hemchandracharya North Gujarat University,Patan
MS University of Baroda
Veer Narmad South Gujarat University
Maharaja Krishnakumarsinghji Bhavnagar University
KSKV Kachchh University, Bhuj
Parul University, Vadodara
Sardar Patel University
Sumandeep Vidyapeeth University (Deemed), Vadodra
PT. B.D. Sharma University of Health Sciences, Rohtak
Maharishi Marakandeshwar University (Deemed), Ambala
Himachal Pradesh University
Maharishi Marakandeshwar University, Kumarhatti, Solan
Jammu University
Kashmir University
Deemed University, Jammu & Kashmir
Kolhan University, Chaibasa
Vinoba Bhave University
Ranchi University
Rajiv Gandhi University of Health Sciences
KLE Academy of Higher Education & Research (Deemed), Belgaum
JSS University, Mysore
Manipal Academy of Higher Education (Deemed University), Manipal
Nitte University (Deemed), Mangalore
Sri Devaraj Urs Academy of Higher Education and Research (Deemed University), Kolar
Sri Siddhartha Academy of Higher Education, Tumkur
Yenepoya University of Health Sciences (Deemed), Mangalore
Kerala University of Health Sciences, Thrissur
Amrita Vishwa Vidyapeetham University (Deemed), Coimbatore
Calicut University
Mahatma Gandhi University, Kerala
Barkatullah University
Madhya Pradesh Medical Science University, Jabalpur
Dr. Harising Gour University,Sagar
Jiwaji University
Devi Ahilya Vishwa Vidyalaya
Rani Durgavati Vishwa Vidyalaya, Jabalpur
Peoples University, Bhopal
Vikram University, Ujjain
AP Singh University
Maharashtra University of Health Sciences, Nashik
Bharati Vidyapeeth University (Deemed), Pune
Dr. D Y Patil University (Deemed), Pimpri, Pune
D.Y. Patil Education Society (Deemed University), Kolhapur
Datta Meghe Instt. of Medical Sciences (Deemed University), Nagpur
Krishna Institute of Medical Sciences University (Deemed), Karad
MGM Institute of Health Sciences (Deemed University), Navi Mumbai
Padmashree Dr. D Y Patil University (Deemed), Navi Mumbai
Pravara Institute of Medical Sciences (Deemed University), Ahmednagar
Manipur University
North Eastern Hill University, Shillong
Utkal University
Sambalpur University
KIIT University (Deemed), Bhubaneswar
Berhampur University
North Odisha University, Baripada, Odisha
Vinayaka Missions University (Deemed), Salem
Statutory Autonomous, Puducherry
Sri Balaji Vidyapeeth (Deemed University), Pondicherry
Bharath University (Deemed), Chennai
Adesh University, Bathinda
Baba Farid University of Health Sciences,Faridkot
Rajasthan University of Health Sciences
Rajasthan University
Jaipur National University (Private Univ.)
NIMS University (Deemed), Jaipur
Pacific Medical University, Udaipur
Sikkim Manipal Univ. of Health,Medical & Tech. Scs
Dr. MGR Educational and Research Institute (Deemed University), Chennai
The Tamilnadu Dr. MGR Medical University,Chennai
Chettinad Academy of Research and Education (Deemed University), Chettinad
Meenakshi University (Deemed), Chennai
Annamalai University
Saveetha University (Deemed), Chennai
Sri Ramachandra University (Deemed), Chennai
SRM Institute of Science & Technology
Kaloji Narayana Rao University of Health Sciences, Warangal
Tripura University
Hemwati Nandan Bahuguna Uttarakhand Medical Education University
Kumaon University, Nainital
Swami Rama Himalayan University, Dehradun
H. N. B Garhwal University
Uttrakhand Technical University, Dehradun
Deen Dayal Upadhyay Gorakhpur University, Gorakhpur
Dr. Ram Manohar Lohia Avadh University, Faizabad
King Georges Medical University,Lucknow
Dr B R Ambedkar University,Agra, U.P.
Glocal University, Saharanpur
Ch. Charan Singh Universitiy, Meerut
C.S.J.M. University, Kanpur
Mahatma Gandhi Kashi Vidyapith, Varanasi
Banaras Hindu University
Integral University, Lucknow
Aligarh Muslim University
Bundelkhand University
M J P Rohilkhand University
Santosh University
Sharda University, Greater Noida
Swami Vivekanand Subharti University, Meerut
Teerthanker Mahaveer University, Moradabad
Dr. Shakuntala Misra National Rehabilitation University, Lucknow
Mahatma Jyotiba Phule Rohilkhand University
Shri Venkateshwara University, Gajraula
West Bengal University of Health Sciences, Kolkata


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